周流的

For suspected cases, in early pregnancy (8-12 weeks), chorionicvilluscell karyotype analysis was done in those with spontaneous natural abortion; in the midtrimester (13-24 weeks) and the late pregnancy (over 25 weeks), the abortus or newborn blood was collected for chromosome analysis.

对有胎儿异常可能的病例，孕早期(8～12周)自然流产者行绒毛细胞核型分析，孕中期(13～24周)及晚期(25周以上)取流产儿和新生儿血进行染色体分析。

With perturbation shifting circumferentially step by step, the subsonic flows around slender bodies of revolution vary gradually in manner of single periodicity,while the supersonic flows vary gradually in manner of double periodicity.

由于临界周向位置的存在，随微小扰动沿周向逐渐移动，细长旋成体大攻角亚音速绕流呈现为"单周期、连续变化"规律，而超音速绕流则呈现为"双周期、连续变化"规律。

The SMMC-7721 hepatoma cell line of experimental groups and control groups were cultured with the addition of sodium selenite, potassium iodide or triiodothyronine (T3). The cell quantity was checked by the MTT assay , and the cell cycle was observed by flow cytometry and levels of AFP and ALB in the culture solution were determined by RIA.The SD rats were divided into positive control group, negative control group, T3 group and mixture group (the mixture of sodium selenite, KI and T3). After two weeks of the administration of sodium selenite、 KI and T3,Walker256 cells were transplanted into the abdominal cavity. The administration was kept on for another two weeks, then observed the morbility and mortality of the rats with ascites carcinoma. At the end, the ascites were for the cells counting and the cell cycles analysising.

方 法：在体外，应用细胞培养的方法，培养SMMC－7721肝癌细胞系，分为试验组和对照组，试验组加入不同浓度Se、I或T3干预，培养一段时间后，通过噻唑蓝试验、流式细胞仪，观察细胞数量及细胞周期的变化，测定细胞培养上清液中甲胎蛋白、白蛋白的水平，观察细胞分化情况；在体内，将试验用SD大鼠分成阴性对照组、阳性对照组、T3组和混合组（为Se、I和T3混合物），首先预防性用药2周，然后给试验组大鼠和阳性对照组大鼠腹腔注射Walker256细胞，制造腹水癌大鼠模型；继续用药2周，观察各组大鼠发病率及死亡率，测量不同时期大鼠血清FT3含量，通过流式细胞仪测量大鼠腹水细胞数及细胞周期变化。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

1 The approach of LVB cannulated by passed retrograde across the aortic valve can be performed easily and reduce LV pre-load and LV work, as well as cardium oxygen consumption significally; 2 This LVB can improve coronary artery perfusion and cardiac function while AOMP can be maintained after LAD occlusion; 3 There has a potential impariment effect on the right ventricular function with excessive amount BFR (over 85%) during LVB, particularly in the present of myocardial ischemia; 4 Roller pump is not suited for a prolonged bypass because of significant damage to the blood system.

由此我们得出以下实验结论：经外周动脉逆行左室置管转流方法操作简便、迅速。能明显减少左室前负荷，减少左室作功，降低心肌耗氧量；能维持严重心脏缺血后的平均动脉压，改善冠脉循环灌注和左室功能，具有临床应用的可行性。85%以上转流率对缺血心脏的心功能有潜在的损害效应。以75%左右转流率较为合适。转子泵不适宜用作长时间转流。

T tube drainage was able to decrease bile viscosity effectively.The number of bacteria decreased significantly until 21 postoperative days.The time that T tube is drawn out is on 14 postoperative days.Bacteria may exist in bile continuously and play a role on relapse of choledochitis and bile duct stone.

胆汁中的细菌和粘度升高在胆管结石形成中起协同作用。T管引流能有效地降低胆汁粘度，而胆汁中细菌量在引流术后第3周才有显著减少，临床2周时拨除T管细菌可潜伏下来，对胆管炎和胆管结石的复发可能起作用。

The mathematic models of damp groove type continuous oil supply port plate /cylinder block and semi-perimeter double oil groove type periodical oil supply port plate /cylinder block frictional pair was established in the paper.

本文对阻尼槽型连续供油配流副和半周槽双油腔间歇供油配流副两种较典型的配流副进行了数学建模。

In the paper, the simulation model of port plate/cylinder block frictional pair in which the lubricating film forms was established according to force balance equation and flow continuity equation with SIMULINK of MATLAB. The frictional pair includes of damp groove type continuous oil supply port plate /cylinder block frictional pair and semi-perimeter double oil groove type periodical oil supply port plate /cylinder block frictional pair.

本文根据配流副数学模型中的力平衡动态方程和流量连续方程，利用数学仿真软件MATLAB中的SIMULINK工具包分别对阻尼槽型连续供油配流副和半周槽双油腔间歇供油配流副建立了润滑膜形成的仿真模型，并对不同工况下的这两种配流副润滑膜的形成及变化规律进行动态仿真研究。

The flow parameters, which controlled the heat transfer phenomena in the coolant channel of the piston, are identified; the experimental facilities and program will be established in accordance with the parametric conditions experienced by a real marine diesel engine. The experimental result for the flow within smooth-walled duct under the reciprocating motion will be used as the reference datum to assess the results for the study of heat transfer augmentation. As an attempt to enhance the cooling performance, the ribs will be installed in the coolant channels which flow was orthogonal mode for the piston moving direction. Finally, the results obtained for the tests with heat transfer augmentation devices will be compared with the smooth-walled test results.

本研究计画利用座标转换理论，对受到与往复运动正交方向之流场进行参数分析，建立控制此流场热传性能之参数后，模拟实际船舶主机运转时活塞之冷却情形并进行实验量测，作为评估活塞冷却之基准，然后应用现行已实用於工业界的技术，在与往复运动正交方向之圆形冷却通道中加装凸起物以增加热传效能并予以测量其热传系数及其相关之物理现象，据以讨论此流场受往复力之影响，在周向不同位置之热传情形以及其与平行於往复运动方向之流场的差异。

My son 5 years old, when two-year-old on the occasional nosebleeds, small, 4-year-old when more than three months on the stream once every 10 days for half a year and a half months ago on the flow time, and also more than the original, to the hospital, said blood tests are normal, the point of medicine at the children do not love the beginning of .8 month almost 32 days on stream, but also very large, went to the hospital check, blood test or normal, open points syrup, and eat Chinese medicine granules, not a week, drug use is also a day on the 15 o'clock a sudden nose bleeding out, exhausted all the methods normally used only finally stopped, six o'clock in the morning and a stream, though not at night, and also have more than the original (the child was afraid I worry that the deal did not tell my own, I was based on the many large blood on the ground and pumping his college paper used judgments), or normal blood, the cream of the point that no matter, I was pleased, but the children come back again every day flow, and sometimes more than once or twice a day, which is a headache for two days and asked him if he do not understand how that specific pain law, is not the teacher the past two days that he said he did not love school is really a headache or a headache, I was to determine if a teacher said he does not love the child's school is a small trick.

5岁男宝宝经常流鼻血，最近两天又说头痛怎么办我的宝宝5岁了，两岁多的时候开始就偶尔流鼻血，量少，4岁多的时候开始三两个月就流一次，半年前开始隔十天半月就流一次，并且也比原来多，到医院验血说都正常，开点上的药水宝宝不爱点。8月分开始几乎三两天就流，还非常多，又去医院查，验血还是正常，开了点药水，和吃的中药颗粒，没到一周，还正用药，一天夜里3点就突然鼻子向外大量流血，用尽了平时用过的所有方法才好不容易止住了，早上六点又流了一次，虽没有夜里多，也是比原来都多(宝宝怕我着急，这次没告诉我自己处理的，我是根据地上的许多大血滴和他用过的纸抽团判断的)，血还是正常，开了点药膏，说没什么事，我挺高兴的，可是宝宝回来又是每天都流了，有时一天还不止一两次，这两天说是头痛，问他又说不明白具体怎么疼法，是不是老师这两天说他他不爱上课就说头痛还是真的头痛，我正在判断中，如果是老师说的他就不爱上课当然是宝宝的小把戏。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。