周流的

Material and Methods:From March,2008 to January,2009,250 patients(146 Males and 104 Females,ranged from 18 to 82 years old,mean age was 48 years old) enrolled in the First Affiliated Hospital of Kunming Medical College were performed dual energy scan(Group A,200 patients) and Neuro-DSA scan(Group B,50 patients) using DSCT.The patients were randomly divided into Group A and Group B.Regarding to the justification of scan protocol,100 patients in Group A were selected as sub-group to explore the scan protocol.The image quality Was analyzed both in four categories(4.0/60、4.5/60、4.0/70、4.5/70) of flow rate and total amount of contrast-enhanced material and in three categories(100—150HU、150—200HU、200-250HU) of CT value of left common carotid artery.The comparison of two scan approach was based on image quality,radiation does, postprocessing methods,time of scanning and subtraction and data storage amount.To validate the diagnostic capability of DE-CTA virtual unenhanced images,100 patients in Group A were undergoing head scan both in routine non-contrast program and DE-CTA virtual unenhanced program.Mean CT values,signal-to-noise ratio,image quality,sensitivity of lesion identification,and radiation does were applied to comparison.According to diagnostic results of dual energy of cerebral arterial angiography,62 patients out of 200 patients in Group A were confirmed with cerebral vessel lesions,the results were complied with DSA findings(the time between the two methods were within 1 week).

材料与方法收集2008年3月到2009年1月间在昆明医学院第一附属医院行DSCT CTA检查者250例(男性146例，女性104例，年龄范围18-82岁，平均48岁)，随机分为A、B两组，A组200例行双能量头颅CTA扫描；B组50例行Neuro-DSA扫描。A组前100例按对比剂不同流率和总量分为4组(4.0/60、4.5/60、4.0/70、4.5/70)对图像质量比较；按触发时左颈总动脉内平均CT位分3组(100—150HU、150—200HU、200-250HU)，比较图像质量的差异；对A、B两组血管图像质量、辐射剂量、后处理的方法、扫描和后处理时间及数据的容量进行比较分析；A组中前100例行常规与DE-CTA虚拟平扫的平均CT值、信号噪声比、图像质量、病灶显示情况、辐射剂量的比较分析；A组中行DSA检查62例(两种方法间隔时间均1周内)，比较两种检查方法诊断结果是否符合，并对其中24例动脉瘤行动脉瘤部位、数目、形态、瘤颈显示程度、瘤颈大小及瘤体长径和短径的比较研究。

At room temperature before 500 μL of PBS was added to make a unicellular suspension.

流式细胞术（flow cytometry， FCM）以其对荧光染色的单细胞悬液进行快速、准确的测量和分析而广泛应用于细胞生物学、肿瘤学、免疫学等领域，特别是多种识别白细胞抗原的单克隆抗体的出现，使FCM 在外周血白细胞分析的基础研究和临床诊断中得到了广泛的应用［1］。

This article selected some archetypically meaningful artists with actual corresponding West Lake landscape paintings as references,including Dai Jin,the founder of "Zhejiang School" in early Ming dynasty;Shen Zhou,the founder of "Wu Schooi" in middle Ming dynasty and Dong Qichang,the leader of "Songjiang School" in late Ming dynasty;Li Liufang,one of "Nine Pals in the Painting";Lan Ying,the leader of "Wulin School" and professor Chen Hongshou who was closely related to the West Lake landscapes.

本文选取了有西湖山水图实物可供参证并极具典型意义的一些画家，包括明代前期&浙派&的开派宗师戴进、明代中期&吴门画派&的开派宗师沈周以及晚明&松江派&的领袖董其昌、&画中九友&之一的李流芳、&武林派&的领袖蓝瑛以及和西湖山水有着密切关联的陈洪绶；清代部分则包含有清前期&娄东派&的领袖王原祁、极受乾隆宠遇的学士画家董邦达以及道光、咸丰年间颇具影响力的戴熙。

Methods:The effect of chrysin on HIV1 infection and replication in vitro were detected by cell fusion of MT2 cells and HIV1ⅢB/H9 cells labeled calceinAM and the inhibition study of syncytia formation and p24 production monitored in culture supernatant of MT2 cells infected by HIV1ⅢB, respectively. Flurescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD69 by activated CD4+T in response to PHA.

以CR对CalceinAM标记的慢性感染HIV1ⅢB的H9细胞（H9/HIV1ⅢB）和MT2细胞的融合、对HIV1诱导的MT2细胞形成合胞体及对感染HIV1的MT2细胞产生p24抗原的影响，探讨其抗HIV1活性；以PHA刺激人外周血单个核细胞，利用流式细胞术，检测CD4+T细胞CD69表达的百分率，分析CR对免疫活化的影响。

The results by invert embedding TEM showed that higher invasive CCL229cells had vast of ER,which distributed throughout cytosol especially largeamount of vesicle-like and flatten cisternal rER in pseudopodia or cell processesand membranous flow-like structure from perinuclear region to pseudopodia,and less Golgi complex.

倒置包埋法常规透射电镜对人大肠癌细胞的超微结构观察结果显示，高侵袭力的CCL229细胞中内质网数量较多并呈全细胞分布，尤其在细胞伸出的众多伪足中含有丰富的小泡状和扁平囊样粗面ER结构，而且观察到由小泡形成的&膜流样&结构从核周一直深入到伪足中，但细胞中高尔基器少见。

The second part was to test the appearance, ingredient analysis, surface potential, thermal conduction properties, and magnetism of the nano composite fluid prepared from the parameters. The experiment found that unoxidized particles, Ag, Cu, Fe, and Ni appeared round. If combined with oxygen in water as oxides, the nano particles would grow toward certain directions. In copper/iron nano composite fluid, FeO appeared cubic, Cu2O appeared coniferous. In silver/iron nano composite fluid, FeO was polygonal. In dielectric potential detection, the nano composite fluid was likely to aggregate and deposit, except for silver/iron set. In other sets, the pH of silver/cobalt nano composite fluid was 7, surface potential was 21.21mV; the pH for silver/nickel nano composite fluid was 6.5, surface potential was 21.04mV; the pH for copper/iron nano composite fluid was 7, surface potential was -30mV. The fluid particles of the three sets could all maintain suspension of 2 weeks or more. For thermal conduction, the silver/nickel, nano composite fluid showed the best thermal conduction. Under temperature of 30℃ fluid weight conduction of 0.4%, the thermal conduction increase was 26%. For magnetic detection, except for silver/iron nano composite fluid, the nano particles of other three sets were paramagnetic, and all four sets were soft magnetic nano composite materials.

第二部分。将由较佳制备参数所产出的奈米复合流体，进行形貌外观、成分分析、表面电位及热传导性质实验与磁性检测；在奈米颗粒的形貌部分，经实验发现Ag、Cu、Fe、Ni等未氧化的颗粒皆呈现近似圆形，而若与水中的氧结合形成氧化物，奈米颗粒则会朝特定方向成长，在铜/铁奈米复合流体中FeO为四方体结构、Cu2O的颗粒是针叶状，在银/铁奈米复合流体中FeO则为多边形结构；而介面电位检测方面，结果显示经本制程所产出的奈米复合流体除银/铁奈米复合流体这一组较容易聚集沉淀外，其他三组中银/钴奈米复合流体的pH值为7，表面电位为21.21mV，银/镍奈米复合流体的pH值6.5表面电位为21.04mV而铜/铁奈米复合流体的pH值为7时表面电位约在-30mV，且此三组流体颗粒皆能维持悬浮性2周以上，悬浮性较佳；热传导实验部分，四组奈米复合流体中以银/镍奈米复合流体在增进热传上效果最佳，在温度30℃及流体重量浓度0.4﹪条件下热传导系数增进达26﹪；磁检测方面，除了银/铁奈米复合流体外，其他三组奈米颗粒皆属顺磁性，且这四组奈米复合材料都是属软磁性。

The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清，进行以下检测：(1)原子吸收法测血清锌浓度；(2)测胸腺、脾脏脏器重量并与大鼠体重比较，计算脏器指数；(3)HE染色对胸腺、脾脏进行病理观察；(4)原位末端标记法测胸腺、脾脏中淋巴细胞的自发凋亡，并通过图像分析进行比较；(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达；(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达；(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

To investigate the expressions of Fas and FasL on tumor cells and TILs in patients with so as to understand the potential role of Fas system in immune escape and counteratteck involved in HCC,the expression levels of Fas and FasL were detected on tumor cells,noncancorous liver cells,TILs and PBLs in patients with HCC by means of indirect fluorescent immunoassay flow cytometry.

为研究肝细胞癌患者肿瘤细胞、肿瘤浸润淋巴细胞Fas抗原与FasL配体的表达水平，探讨Fas系统在肝细胞癌免疫逃逸及免疫反击中的作用，采用间接免疫荧光流式细胞术检测20例肝细胞癌患者肿瘤细胞、肿瘤浸润淋巴细胞和外周血淋巴细胞Fas与FasL的表达。

There prominent suppression of cellular immunity in secondary syphilis patients is observed and the change of T-lymphocyte subsets and NK cells were closely with the pathogenic mechanism of syphilis.

我们应用流式细胞仪对Ⅱ期梅毒患者外周血T淋巴细胞亚群、NK细胞、B细胞进行检测并与正常组对照，了解梅毒患者的细胞免疫功能状态，探讨其在梅毒的发生、发展中的作用及意义。

This experiment selects material E14d SD the big mouse SVZ nerve stemcell as well as P7d the SD big mouse internal ear stem cell, does nothave the blood serum complete culture medium raise through D-MEM/F12to cause its massive multiplication, a part carries on Nestin, NF,CNP, GFAP, MAP-2, Myosin, the P27 immunity group, proved the raise thenerve stem cell has many to the split up ON potential; Another partdyes EGFP after the extension to carry on the guinea pig internal earto transplant, after two weeks fills the class to take the cochleafixed slice observation, discovered has the stem cell ball group inthe internal ear survival, proved the nerve stem cellheterotransplantation the possibility and for transplants the stemcell migration differentiation repair hair cell damage the thoroughresearch accumulation experiment experience.

本实验取材E14d SD大鼠SVZ神经干细胞以及P7d SD大鼠内耳干细胞，通过D-MEM/F12无血清完全培养基培养使其大量增殖，一部分进行Nestin、NF、CNP、GFAP、MAP-2、Myosin、P27免疫组化，证明培养的神经干细胞具有多向分化的潜能；另一部分通过转染EGFP后进行豚鼠内耳移植，两周后灌流取耳蜗固定切片观察，发现有干细胞球团在内耳存活，证明神经干细胞异体移植的可能性并为移植干细胞迁移分化修复毛细胞损伤的深入研究积累实验经验。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。