周流的

Isolation, cultivation and identification of human embryonic NSC from cerebral cortex of 7-12 week abortus. By using flow cytometry and MTT assay, the effects of various concentration of TSPG and TSPG cooperating with cytokines in NSC culture media for 3 days on proliferation of human embryonic NSC has studied. By employing immunocytochemistry assay of the expression of tyrosine hydroxylase, the effect of different dilution of TSPG and TSPG cooperating with IL-1 on induced differentiation of human embryonic NSC into dopaminergic neuron has researched.

从7～12周人工流产胚胎脑组织中分离培养人胚胎NSC，免疫细胞化学鉴定NSC特异性nestin抗原表达，在NSC的培养体系中加入TSPG，采用流式细胞术和MTT法检测不同浓度TSPG以及TSPG与EGF，bFGF联合应用对人胚胎NSC增殖的影响；经免疫细胞化学检测培养细胞酪氨酸羟化酶表达，分析不同浓度TSPG以及TSPG与IL-1联合应用对NSC定向诱导为DA能神经元的影响。

RESULT: The activity and drinking of mice who had proceeded bone marrow transplantation after 60Co total body homogeneous radiation was no significantly different with normal mice, but their pelage became grey, blood samples detection were also same with normal mice after five weeks of bone marrow transplantation, the proportion of GFP positive cells in bone marrow cells achieved 59.62±13.21% proved by laser confocal microscopy, all these evidence proved the bone marrow reconstitution had been finished in the recipients.

结果： 60Co全身均匀照射后进行骨髓细胞移植的骨髓移植组小鼠的活动与饮食与正常小鼠无明显差别，只是毛发渐变灰白，五周后其血常规与正常鼠相较无明显差异，流式细胞仪检测显示其骨髓细胞中GFP阳性细胞的比例也达到59.62±13.21%，提示骨髓重建成功。

These experiments show that phycoerythrin conjugates are a valuable class of fluorescent reagents for analysis of cell surface antigens.phycoerythrin conjugates can be used together with fluorescence-labeled reagents for two-color analysis.

结果 用R-PE标记的抗CD 3 、抗CD 4 、抗CD 8 抗CD 19 、抗CD 45 等单抗试剂，检测正常人外周血白细胞表面相应抗原的表达，流式细胞仪分析表明，R-PE标记的抗体特异性保持完好，且荧光强度高，还可配伍成双标试剂。

At 24 hours after transplantation, the presence of donor cells were analyzed by flow cytometry. Blood was obtained from the mouse caudal vein after transplantation to analyze the changes in number of leukocytes, erythrocytes and thrombocyte in peripheral blood.

移植后24 h，应用流式细胞仪分析骨髓中供体细胞的含量，或在移植后定期从小鼠的尾静脉取血，分析外周血中白细胞、红细胞及血小板的数量变化。

Patients were consideredpositive when the expression were more than 20% cutoff for ZAP-70 and30% cutoff for CD38 .The CDS+ T cells in the same sample were used asinternal positive control, Isotype control was use for every sample .serumβ2-microglobulin was analyzed by ratenephelometry.

应用四色流式细胞术检测50例B-CLL患者外周血白血病细胞ZAP-70和CD38的表达，以ZAP-70≥20％、CD38≥30％为阳性，以CD5~+的自身T细胞为内在阳性对照，同时作同型对照。

Bronchial carcinoid tumors may be manifested by severe and prolonged flushing episodes, facial and periorbital edema, excessive lacrimation, salivation, tachycardia, and hypotension.

支气管类癌综合征可以表现为严重的长时间的潮红、面部和眶周水肿、多泪、流涎、心动过速或低血压。

However, the effect from impeller did not keep to the outlet of the vaneless diffuser. Through analyzing the periodic mean field, an unsteady effect region was found within the region whose biggest radius ratio was less than 1. 2 in the vaneless diffuser. The unsteady flow at three flow rate conditions had the similar unsteady characteristic.

无叶扩压器内近前盘r-6面上锁相平均流场的分析表明，当叶轮处于不同周向位置时，无叶扩压器内的流动表现出非定常流动特性，尤其是在人口区域非定常流动特性更为明显。

This can be a really bad ascension day or week where one may feel quite ill until the discord is cleared that has surfaced within the DNA and energy flow.

这会是一个糟糕的提升日子或提升一周，其中你会感到非常不舒服，直到在DNA和能量流中浮现出来的不和谐被清理掉为止。

METHODS: After isolated the PBMCs and blood-plasma from adult human peripheral blood by Ficoll-Hypaque centrifugation, the PBMC culture procedure with or without the self-blood -plasma was applied to polarize T cells in vitro , these cells were polarized by PHA(20 mg/L),non-PHA respectively.

用Ficoll-Hypaque梯度离心法分离出正常成人抗凝外周血单个核细胞和血浆，将PBMC置于含或不含有自身血浆环境中，并在植物血凝素(PHA ，2 0mg L)的刺激下进行培养，培养 2 4、48、72h后收获细胞，分别用双色免疫荧光标记，以流式细胞术对CD4+ T细胞表达表面分子CCR3、CCR5进行分析。结果：CD4+ T细胞受自身血浆和PHA刺激后向Th1 Th2两个方向极化，2 4、48、72h后极化率分别达到(13 2 8± 1 5 9)%(12 70± 1 6 5 )%、(17 19± 1 0 3)%(17 5 0± 1 30 )%、(19 49± 2 87)%(18 5 8± 1 49)%；而只有PHA刺激的极化率很低，两组有显著性差异(P 0 1)；Th1∶Th2之比约等于 1。

Methods Flow cytometry was used to detect CD14highCD16- and CD14+CD16+ monocyte subpopulations in peripheral blood from 58 patients with untreated syphilis, including 36 cases of latent syphilis,8 cases of primary syphilis and 14 cases of secondary syphilis, as well as from 65 normal human controls.

流式细胞仪检测58例未经治疗的梅毒患者（包括隐性梅毒36例，一期梅毒8例，二期梅毒14例）的外周血单核细胞CD14highCD16-亚群和CD14+CD16+亚群。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。