周流

These experiments show that phycoerythrin conjugates are a valuable class of fluorescent reagents for analysis of cell surface antigens.phycoerythrin conjugates can be used together with fluorescence-labeled reagents for two-color analysis.

结果 用R-PE标记的抗CD 3 、抗CD 4 、抗CD 8 抗CD 19 、抗CD 45 等单抗试剂，检测正常人外周血白细胞表面相应抗原的表达，流式细胞仪分析表明，R-PE标记的抗体特异性保持完好，且荧光强度高，还可配伍成双标试剂。

Methods To review the 72 cases of high perianal abscess in three years. 49 cases were received seton and incision drainagetherapy.

回顾性 总结了 3 年来治疗的 72 例高位肛周脓肿病例，其中 49 例切开挂线术，23 例行单纯性切开引流术。

Methods The peripheral blood lymphocytes were stimulated by Stimulator in order to enhance expression of cytokines .

先用刺激物刺激外周血T细胞，以增加细胞内细胞因子的表达，继用流式细胞仪分析特异性细胞因子表达水平。

The expression of CD8+、CD28+ on peripheral blood lymphocytes in patients with gastric cancer is down and T cell subpopulation is abnormal,which may plays an important role in the pathogenesis of gastric cancer.

我们用流式细胞仪检测胃癌患者外周血的CD28和T淋巴细胞亚群的表达以及和临床病理分期的关系，以探讨胃癌患者的免疫功能变化，并为肿瘤的免疫逃逸机制提供研究依据。1对象与方法1.1对象36例胃

Both methods are advanced flow cytometry methods, the results of two groups are correlated. The two methods can be substitutable, and used to perform the same assay.

在严格按操作规程进行校准和质控在控的前提下，使用这2种流式细胞检测系统测定外周血T细胞亚群的结果基本一致。

In the circle passage, the pressure is lowest at the point 30～60°away from the inlet of the distributor and the component velocity in tangential direction is greater than that in radial direction.

环形流道内周向压强分布呈非单调变化，在距离气体进口30～60°处压强最低。

Methods Three-color fluorescent flow cytometry was used to analyze the percentage of peripheral blood T cell subsets in the young and elderly groups.

方法采用三色免疫荧光标记流式细胞术分析年轻组(21~59岁)与老年组(≥60岁)外周血T淋巴细胞亚群水平。

At 24 hours after transplantation, the presence of donor cells were analyzed by flow cytometry. Blood was obtained from the mouse caudal vein after transplantation to analyze the changes in number of leukocytes, erythrocytes and thrombocyte in peripheral blood.

移植后24 h，应用流式细胞仪分析骨髓中供体细胞的含量，或在移植后定期从小鼠的尾静脉取血，分析外周血中白细胞、红细胞及血小板的数量变化。

At room temperature before 500 μL of PBS was added to make a unicellular suspension.

流式细胞术（flow cytometry， FCM）以其对荧光染色的单细胞悬液进行快速、准确的测量和分析而广泛应用于细胞生物学、肿瘤学、免疫学等领域，特别是多种识别白细胞抗原的单克隆抗体的出现，使FCM 在外周血白细胞分析的基础研究和临床诊断中得到了广泛的应用［1］。

Methods The discharge features of hippocampus CA1 pyramidal neurons of 14-day-aged healthy Sprague-Dawley rats induced by low dose (0.3~0.8mol/L) of veratrine were observed by slice patch-clamp technique.

应用脑片膜片钳全细胞记录技术，观察小剂量(0.3~0.8mol/L)Ver灌流后SD大鼠（2~3周龄，共30只）海马CA1锥体神经元的放电特征。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。