周流

Emergency heart and lung repair plus pericardiac drainage were carried out via left anteriolateral incision in 6 cases and right anteriolateral incision in 1 case.

结果 本组术前术中输血400～2000ml，术后输血400～600ml，均止血彻底，无二次开胸。5～11d心肌酶恢复正常，5～14d拔除引流管，7例均痊愈，出院后2周、6周复诊无胸腔积液、肺不张。

Medication during early pregnancy can cause fetal spina bifida, cleft palate, clubfoot, small limbs, Phocomelia, umbilical prominent cheek transverse fissure, cerebral meningocele, cerebral spinal cord dysfunction, osteogenesis imperfecta and fetal death. 2, prednisone: big neat administration during early pregnancy can cause stillbirth, streaming, no brains infants, small ectopic kidney, one-eyed, cleft palate, bone deformities, such as. 3, 6-8 weeks pregnant villus chromosome examination; pregnant 16-20 weeks to do the amniotic fluid puncture in amniotic fluid cell chromosome examination; At the same time, dynamic observation of maternal alpha-fetoprotein content; attention fetal B-track general shape and structural abnormalities offal.

妊娠早期用药可致胎儿脊柱裂，腭裂，畸形足，小肢，短肢畸形，脐突出，颊横裂，脑脊膜膨出，脑脊髓功能障碍，成骨发育不全及胎儿死亡。2、强的松：妊娠早期大齐整给药可引起死产、流、无脑儿、小异位肾、独眼、腭裂、骨畸形等。3、孕6-8周取绒毛组织进行染色体检查；孕16-20周行羊水穿刺做羊水细胞的染色体检查；同时，动态观察母血中的甲胎蛋白含量；注意B超跟踪胎儿大体形态和内脏结构是否异常。

Strict observation and nursing care of the drainage after Pelvic lymphadenectomy and the prompt note of symptoms and signs play a very important role in preventing the formation of postoperative lymphocysts.

术后1周发生盆腔淋巴囊肿的有45例，术后2周发生的有48例，囊肿直径最小的7 mm，最大的150 mm。结论：做好盆腔淋巴结切除术后引流管的护理，重视早期症状，对预防淋巴囊肿的发生发展有着非常重要的意义。

Objective In order to know the essential meaning of immune disorder in Recurrent Aphthous Ulcer patients and to search the agreeable treatment meanwhile, the cluster of differentiation of peripheral lymph cells were detected and analyzed by flow cytometry, and afterwards those patients were treated by immunomodulator.

目的为了更好的理解RAU患者免疫紊乱的实质及寻找有效的治疗方法，我们采用流式细胞技术，对RAU患者发病期和康复期外周血淋巴细胞的表型进行测定、分析，探索流式细胞技术在RAU的诊断及指导临床治疗方面的作用。

Refer to the domestic and foreign literature material, to establish traumatic joint stiffness model with New Zealand big white rabbit;caused the right knee joints of the big white rabbits closed trauma with the appropriate mothod,then fixed the behind-right knee joints 4 weeks with the gypsum and the bandage;after 4 weeks, removed fixture,examined the animal blood rheology,the joint range,the skin temperature, the arthrocele extent and so on ,to authenticate whether modeling succeeded or not.

参考国内外文献资料，用新西兰大白兔建立创伤性关节僵直模型；用适当的方法造成大白兔右后膝关节闭合性损伤，用石膏绷带、绷带固定4周，4周后拆除固定。期间观察皮肤温度、关节肿胀度、关节活动度，检测动物血液流变学等指标，以验证模型建立是否成功。

Bacteritic bile viscosity increased but bacteria were not the cause that resulted bile viscosity elevation.

有菌胆汁菌落量在术后2周内不随引流时间增加而变化，直到术后第3周菌落量才有显著减少。

Fluid field tests and numerical calculation have been carried out on an excellent shipform DO11 with an extrorse cochlea-channeled stern .

对长江优秀船型东方红11号(DO11)及其外旋蜗尾改型进行了计算，结果表明：DO11外蜗改型船桨盘面处的周向和径向合成伴流计算结果与流场测量结果定性一致。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

METHODS: T-lymphocyte subsets and NK activity were determined by flow cytometry in 78 patients with hypopharyngeal squamous cell carcinoma. Blood samples of 20 non-tumor patients were used as control.

用流式细胞仪对78例喉咽鳞癌患者的外周血进行T淋巴细胞亚群及NK细胞活性的检测，并取20例非肿瘤患者的外周血作为对照组。

Methods: The expression of CD28, CD80 and CD86 were detected in lesional skin in 22 and in circulating lymphocytes in peripheral blood in 17 patients with psoriasis by immunohistochemical technique and flow cytometry respectively.

方法采用免疫组化ABC法检测22例银屑病皮损、流式细胞仪检测17例银屑病外周血淋巴细胞上CD28、CD80、CD86的表达水平。15例整形外科手术病人的皮肤和外周血作正常对照。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。