周流

METHODS Interferon-γ and interleukin 4 (IL-4) secreted by CD 3 +, CD 8 -T cells were detected by flow cytometry in 38 patients with secondary syphilis and 20 healthy volunteers, and significance of TH1/TH2 cell ratio in the etiology of syphilis was analyzed.

采用流式细胞分析技术检测 38例Ⅱ期梅毒患者和 2 0例健康体检者外周血CD3 +、CD8-T细胞胞内细胞因子IFN γ和IL 4的表达。

There prominent suppression of cellular immunity in secondary syphilis patients is observed and the change of T-lymphocyte subsets and NK cells were closely with the pathogenic mechanism of syphilis.

我们应用流式细胞仪对Ⅱ期梅毒患者外周血T淋巴细胞亚群、NK细胞、B细胞进行检测并与正常组对照，了解梅毒患者的细胞免疫功能状态，探讨其在梅毒的发生、发展中的作用及意义。

This experiment selects material E14d SD the big mouse SVZ nerve stemcell as well as P7d the SD big mouse internal ear stem cell, does nothave the blood serum complete culture medium raise through D-MEM/F12to cause its massive multiplication, a part carries on Nestin, NF,CNP, GFAP, MAP-2, Myosin, the P27 immunity group, proved the raise thenerve stem cell has many to the split up ON potential; Another partdyes EGFP after the extension to carry on the guinea pig internal earto transplant, after two weeks fills the class to take the cochleafixed slice observation, discovered has the stem cell ball group inthe internal ear survival, proved the nerve stem cellheterotransplantation the possibility and for transplants the stemcell migration differentiation repair hair cell damage the thoroughresearch accumulation experiment experience.

本实验取材E14d SD大鼠SVZ神经干细胞以及P7d SD大鼠内耳干细胞，通过D-MEM/F12无血清完全培养基培养使其大量增殖，一部分进行Nestin、NF、CNP、GFAP、MAP-2、Myosin、P27免疫组化，证明培养的神经干细胞具有多向分化的潜能；另一部分通过转染EGFP后进行豚鼠内耳移植，两周后灌流取耳蜗固定切片观察，发现有干细胞球团在内耳存活，证明神经干细胞异体移植的可能性并为移植干细胞迁移分化修复毛细胞损伤的深入研究积累实验经验。

Studies show that people severely ill with H1N1 flu generally need a week to two weeks on ventilators to recover.

而研究显示，正常情况下甲流病人都需要使用呼吸器一到两周才能康复。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。