周流

The results show that, when the computational error and its accumulation are eliminated effectively, the laminar flow in OFR with perfect-symmetric boundaries behaves in a pattern of central symmetry and circumferential uniformity while the turbulent flow field presents a centrally symmetric and circumferentially non-uniform structure with 8 transverse vortices arrayed circumferentially in a way of central symmetry, similar to Taylor vortices in Couette flow.

模拟结果表明在有效消除计算随机误差以及误差积累的情况下，几何边界理想对称OFR内的三维层流流场呈现出良好的中心对称性和周向均匀性，而湍流流场中心对称的同时呈现出周向非均匀结构－在圆周上生成8个中心对称排列的横向漩涡，其形态与Couette流中的泰勒涡类似。

The person suffers to mixed air, between ground of unripe Yu Tian, with heaven and earth 2 body are not had first, be like what can realize scope of operation is clever, this heart develops lonely of empty crystal clear, natural a fit is circumfluent at fluctuation, open a gas to go out, entire to be enraged, air vents to be like what the ground enrages to rise, gas drops into what be like weather, can be the same as with heaven and earth oneself its are long.

人受冲和之气，生于天地间，与天地初无二体，若能悟天地之妙，此心冲虚湛寂，自然一气周流于上下，开则气出，阖则气入，气出则如地气之上升，气入则如天气之下降，自可与天地同其长久。

With perturbation shifting circumferentially step by step, the subsonic flows around slender bodies of revolution vary gradually in manner of single periodicity,while the supersonic flows vary gradually in manner of double periodicity.

由于临界周向位置的存在，随微小扰动沿周向逐渐移动，细长旋成体大攻角亚音速绕流呈现为"单周期、连续变化"规律，而超音速绕流则呈现为"双周期、连续变化"规律。

And then, in this part, following the idea of Topping [51, 52], we will show an isoperimetric inequality (6.3.1) on surfaces of non-positive Gaussian curvature by means of the curve shortening flow on surfaces, this inequality can be considered as a generalization of the Banchoff-Pohl inequality in the Euclidean plane.

然后，仿照Topping[51]和[52]利用平均曲率流证明等周不等式的想法，我们利用曲面上的曲线缩短流在Gauss曲率非正的曲面上证明了一个等周不等式(6.3.1)，它可以看成欧氏平面上Banchoff-Pohl [8]不等式在曲面上的推广。

Before treatment all patients were hemospasia detected peripheral lipid and hemorheology in early morning;treatment group was given Chinese medicine of eliminating phlegm and stasis,after two weeks they were hemospasia detected peripheral lipid and hemorheology in early morning again.

入院后治疗前均晨起空腹抽取外周静脉血检测血脂六项及血液流变学；治疗组服用祛瘀化痰中药2周后再次晨起空腹抽取外周静脉血检测血脂六项及血液流变学。

The SMMC-7721 hepatoma cell line of experimental groups and control groups were cultured with the addition of sodium selenite, potassium iodide or triiodothyronine (T3). The cell quantity was checked by the MTT assay , and the cell cycle was observed by flow cytometry and levels of AFP and ALB in the culture solution were determined by RIA.The SD rats were divided into positive control group, negative control group, T3 group and mixture group (the mixture of sodium selenite, KI and T3). After two weeks of the administration of sodium selenite、 KI and T3,Walker256 cells were transplanted into the abdominal cavity. The administration was kept on for another two weeks, then observed the morbility and mortality of the rats with ascites carcinoma. At the end, the ascites were for the cells counting and the cell cycles analysising.

方 法：在体外，应用细胞培养的方法，培养SMMC－7721肝癌细胞系，分为试验组和对照组，试验组加入不同浓度Se、I或T3干预，培养一段时间后，通过噻唑蓝试验、流式细胞仪，观察细胞数量及细胞周期的变化，测定细胞培养上清液中甲胎蛋白、白蛋白的水平，观察细胞分化情况；在体内，将试验用SD大鼠分成阴性对照组、阳性对照组、T3组和混合组（为Se、I和T3混合物），首先预防性用药2周，然后给试验组大鼠和阳性对照组大鼠腹腔注射Walker256细胞，制造腹水癌大鼠模型；继续用药2周，观察各组大鼠发病率及死亡率，测量不同时期大鼠血清FT3含量，通过流式细胞仪测量大鼠腹水细胞数及细胞周期变化。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

No Ⅲ° atrioventricular conduction block happened, 9 patients developed right bundle branch block, and 2 patients showed junctional rhythm. Two patients with perimembranous extending outlet were repaired because of residual shunt(0.4cm,0.3cm) on the anterosuperior rim of defect. One patient with trivial residual shunt(0.15cm) on the posteroinferior rim of defect was found closed spontaneously six months later. Tricuspid valve was incised in 10 patients of VSD sextending outlet,8 patients vertical to the valve ring,2 patients parallel to the valve ring. Moderate tricuspid valve regurgitation was found in 1 patient, mild in 5 patients, trivial in 4 patients. No one aggravated. One patient complicated with pericardial effusion.

无Ⅲ°房室传导阻滞发生，9例发生右束支传导阻滞，2例结性心律。2例膜周偏流出道型VSD患者因前上缘残余分流分别为0.4cm和0.3cm，再次手术修补；1例后下缘残留细束样分流，直径0.15cm，随访6个月后自愈；10例膜周偏流出道型VSD患者剪开三尖瓣，8例垂直瓣环，2例平行瓣环；1例三尖瓣中度反流，随访无加重；5例轻度反流，4例轻微反流，随访均无加重；1例心包积液；1例再次进入手术室止血。

Methods In cases of SAP with severe pancreatic and peripancreatic infection, open surgical debridement and drainage was done, and, about one week later, the drainage tubes were removed and a choledoscope was then used repeatedly to debride and wash out the necrotic and infected foci that were hard to drain out.

对于SAP已形成严重胰周感染的患者，在开腹手术清创、引流约1周后，拔除腹腔引流管，顺引流管窦道，插入胆道镜；应用胆道镜对感染灶内难以引流至体外的坏死组织及脓液进行反复冲洗及清除。

My son 5 years old, when two-year-old on the occasional nosebleeds, small, 4-year-old when more than three months on the stream once every 10 days for half a year and a half months ago on the flow time, and also more than the original, to the hospital, said blood tests are normal, the point of medicine at the children do not love the beginning of .8 month almost 32 days on stream, but also very large, went to the hospital check, blood test or normal, open points syrup, and eat Chinese medicine granules, not a week, drug use is also a day on the 15 o'clock a sudden nose bleeding out, exhausted all the methods normally used only finally stopped, six o'clock in the morning and a stream, though not at night, and also have more than the original (the child was afraid I worry that the deal did not tell my own, I was based on the many large blood on the ground and pumping his college paper used judgments), or normal blood, the cream of the point that no matter, I was pleased, but the children come back again every day flow, and sometimes more than once or twice a day, which is a headache for two days and asked him if he do not understand how that specific pain law, is not the teacher the past two days that he said he did not love school is really a headache or a headache, I was to determine if a teacher said he does not love the child's school is a small trick.

5岁男宝宝经常流鼻血，最近两天又说头痛怎么办我的宝宝5岁了，两岁多的时候开始就偶尔流鼻血，量少，4岁多的时候开始三两个月就流一次，半年前开始隔十天半月就流一次，并且也比原来多，到医院验血说都正常，开点上的药水宝宝不爱点。8月分开始几乎三两天就流，还非常多，又去医院查，验血还是正常，开了点药水，和吃的中药颗粒，没到一周，还正用药，一天夜里3点就突然鼻子向外大量流血，用尽了平时用过的所有方法才好不容易止住了，早上六点又流了一次，虽没有夜里多，也是比原来都多(宝宝怕我着急，这次没告诉我自己处理的，我是根据地上的许多大血滴和他用过的纸抽团判断的)，血还是正常，开了点药膏，说没什么事，我挺高兴的，可是宝宝回来又是每天都流了，有时一天还不止一两次，这两天说是头痛，问他又说不明白具体怎么疼法，是不是老师这两天说他他不爱上课就说头痛还是真的头痛，我正在判断中，如果是老师说的他就不爱上课当然是宝宝的小把戏。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。