周期的

A bank looks for guaranty when the business to be financed is too risky: an unrealistic business plan, a managerial team without experience in competitive markets, an industry with problems (fixed telephony is in descending part of its lifecycle!), the presence or interference of state in business.

当商业融资风险较大时银行需要担保：一份不切实际的商业计划，在竞争市场上没有相关经验的管理团队，有问题的行业（固定电话已经到了生命周期的衰退阶段），商业运作中政府的介入或干涉。

Firstly, a new design method of cam profile has been developed by using finite terms of Fourier Series in the cycle of cam angle as the basic equation of cam profile, so the difficulty of base circle wave existing in the method of resonant cam design can be eliminated. On the other hand, the method proceeds from the acceleration of cam profile, which makes the acceleration not only smooth and continuous, but also easy to be controlled, and the cam profile with good performance can easily be acquired.

此方法用以凸轮包角为周期的富立叶级数有限项作为凸轮型线的基函数，可以免去谐波凸轮设计时控制基圆波动的困难；此方法又是从凸轮型线的加速度曲线入手进行设计，使型线加速度不但光滑、连续，而且形状容易控制，便于设计出动力性能优良的凸轮型线。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Department determine the attendance situation of current period at the 20th of every month, collect, check and count the personal punch record registered by the Punch-Card Machine and the APPLICATION TO WORK OUT OF THE COMPANY,BUSINESS TRIP AUTHORIZATION REQUEST and ASK FOR DAY OFF, as occasion requires, publish the employee's absence of duty situation of current month, and figure up the salary referring to the monthly attendance situation.

人力资源行政部每月20日前确认此周期的考勤情况，将考勤机上的个人刷卡记录与员工的《市内外出办事申报单》、《商务旅行授权申请》和《请假申请》进行汇总核对统计，并在必要时，就员工在当月的缺勤情况进行公布，并根据员工的月度考勤情况核算工资。

The real tangential velocity fluctuation indicated that there existed an instability of swirling flow in the cyclone.This instability resulted in the rotation center of the swirling flow deviating from the geometric center of the cyclone,which produced the quasi-periodic oscillation of swirling flow and gave the real tangential velocity fluctuation.

瞬时切向速度的变化反映了旋风分离器内旋转流的不稳定性，这种不稳定性导致了旋风分离器内旋转流的旋转中心与几何中心不重合，旋转中心围绕着几何中心摆动，由此造成了瞬时切向速度随时间的类周期的波动变化。

The inhibition of SPG-Rg3 on the growth of MCF-7 cells was detected by MTT assay,IC50 was calculated to obtain the effective concentration; flow cytometry was used to observe the cell cycle of MCF-7 cells; AO/EB fluorescence double-dye technology was performed to observe the apoptosis of cells in morphology,immunocellularchemistry and RT-PCR were utilized to investigate the apoptosis of MCF-7 cells and its relationship with caspase-8 from protein level and molecule level.

采用MTT法观察SPG-Rg3对MCF-7细胞生长的抑制作用，并计算出IC50，依据IC50值确定SPG-Rg3的有效浓度；流式细胞术检测SPG-Rg3作用后MCF-7细胞周期的变化；AO/EB双染从形态学上观察SPG-Rg3对MCF-7细胞凋亡的作用；免疫细胞化学染色和RT-PCR技术分别从蛋白水平和分子水平上检测SPG-Rg3对MCF-7细胞的诱导凋亡作用及其与caspase-8基因的关系。

The distribution of the growth-associated protein (GAP-43) which is close related to the axon regeneration was examined by immunocytochemical method in the adult rat brain and the rat parietal lobus injuried into which the fetal cerebral suspension was lransplanted.

在发现流式细胞计可用于神经科学的研究后，用FCM研究了大鼠脑发育过程中细胞周期的变化和胚胎大脑细胞移植到成年大鼠顶叶后对宿主顶叶脑细胞分裂的影响；由于生长相关蛋白(GAP-43)与轴突再生密切相关，用免疫细胞化学法研究了GAP-43在成年大鼠脑内的分布及顶叶损伤植入胚胎大脑细胞后GAP-43的变化。

Although there is no one source for all Wiccan liturgy, many liturgical items such as the methods for casting the circle, the Charge of the Goddess,certain myths and expressions are common to many traditions.

虽然对为所有的巫术崇拜者礼拜仪式没有一个来源，许多礼拜式的项目例如投射周期的方法，&女神的掌控，&某种神话和表达对许多传统是共同的。

If the parameters for the function require more than seven registers, the additional parameters are stored on, and passed to the called function, using the stack - a cycle-intensive process that degrades performance and leads to increased RAM usage.

如果函数所用到的参数需要7个以上的寄存器，那么这些多出来的参数就被存储，并且通过堆栈传给调用函数-需要1个周期的过程，这样做降低了性能并且增加了对RAM的使用。

In 2006, the flavoring essence and spice trade development will present what kind of situation?

由于世界经济的快速发展，产品生命周期的普遍缩短，使得预测产品的未来趋势变得愈发困难，但人们对健康的强烈追求将使健康理念继续发挥强大的指挥棒作用，也使保健食品、功能食品和减肥食

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了