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Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆内再移向质膜内外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。

In balance the good pattern for the semi-continuos culture of Pavlova viridis in photobioreactor is obtained under the conditions of 30% renewal rate and 1d nenewal period.

综合考虑各指标,以30%的更新率和1d为更新周期是绿色巴夫藻光生物反应器半连续培养的一个较佳模式。

For the incidence of malaria, its period of cyclic oscillation was about one year, which was in agreement with the result of the secular supervision.

疟疾发病率周期性变化的周期约为1年,这与长期监测的结果相一致;而1%左右的增加率可能是由于近几年人口流动激增和严重的环境污染造成的。

The results show that the fluctuation of the light field can be squeezed periodically. The initial occupancy of the BEC atomic levels has great influence on the squeezing properties of light and the period of oscillation of the squeezing is relating to the circle frequency of the light.

结果表明:光场在与原子BEC相互作用过程中呈现周期性压缩, BEC原子能级的初始占据率对光场的压缩特性具有重要影响,光场压缩的振荡周期与光场圆频率有关。

In the experiment the time-average coolant mass flux is varied from 300 to 400 kg/m2s and the amplitude of the coolant mass flux oscillation is fixed at 0%, 5% and 10% of . The period of the coolant mass flux oscillation varies from 10s to 30s.

在实验参数范围上,介电液FC-72平均质量通率从300到400 kg/m2s而且流量的振荡振幅为0%、5%、10%,流量的振荡周期为10秒到30秒。

Specific siRNA was designed and synthesized, and transfected Hela with liposome siNEO FX, then analyse the role of siRNA on proliferation vitality outside the Hela cells, cells periodic distribution by MTT method, double-layer soft agar clone-forming assay, and flowing cell technology.

设计并合成siRNA,脂质体siNEO FX转染Hela细胞,分别用MTT法,双层软琼脂克隆形成试验和流式细胞术分析了siRNA对Hela细胞体外生长增殖活力、细胞周期分布的作用。siRNA作用后的Hela细胞增殖速度减慢,软琼脂克隆形成率降低,G0/G1,期细胞比率增加。

This method has a number of weaknesses such as random deviation, no quantitative analysis, and a longer testing cycle. The latter is most used to check the crack and slag inclusion inside the solid rocket engine.

该方法存在误检率高、无法定量分析、检测周期长等缺点;另一种是X射线投影技术,主要用于检测发动机第二、三界面的脱粘及药柱内的裂纹、夹渣等缺陷。

Result:Compared with the conventional extracting process of single pot,the extracting process of pot groups dynamic refluence get higher pickup rate of effective components,save energy and shorten the period of manufacturing.

结果:罐组式动态逆流提取工艺与传统的单罐提取工艺相比较,有效成分提取率较高,并且节省能源、缩短生产周期。

Methods Morphologic change of cells were observed with fluorescence microscope;MTT assay was used to evaluate survival rates Survival rates of GLC-82 cells;The influence of NA to cell migration was analyzed by scarification test; The percentage changes of apoptosis and the distributional percentage of cell cycle were analyzed with flowcytometry.

荧光显微镜观察细胞形态的变化;MTT 法检测不同浓度烟酰胺培养细胞后细胞的存活率;划痕试验检测不同浓度的烟酰胺对细胞愈合力的影响;流式细胞仪检测细胞周期和凋亡率。

This pupil filter can realize different types of superresolution by changing the periods of sine function or the transmittance of the central point.

选择正弦函数的周期以及光瞳中心点透过率变化两个参量来实现对最终超分辨效果的调节。

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