周期率

The plasmas collectively oscillated under the effect of the sheath alternating-electric field, whose oscillation cycle is consistent with RF.

系统中的等离子体在鞘层交变电场的作用下在系统中集体振荡，振荡周期与射频频率相符。

Appraising two groups ovulated rate and pregnancy rate,and detect FSH、LH、E2、P at sixteen to eighteen days of menstrual cycle.

评价两组排卵率及受孕率，同时在月经周期的第16～22天抽血查FSH、LH、E2、P。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

The main content included in the paper is as follows: The European identification methods of type of wave load is introduced and compared with the methods in Code of Hydrology for Sea Harbour. In order to reveal their difference, they are tested by the experiment data. The relation between phase and frequency are analysed through the Hilbert and wavelet transformations. And a new method of identifying the breaking wave load is set up based on the linear relation between wave height and wave press before the breakwaters. The probability distribution is tested by the statistic of experiment data. Based on the experiment data, the paper analysed the influence of reflection coefficient according to different factors, and its property is got. The work property of charmfered breakwater under breaking waves is analysed. Through the statisticof its wave press and wave force the distribution of press on the breakwater front face and its reducing effect to breaking wave force are proved.

本文基于此，通过实验研究，主要作了如下工作：介绍了欧洲波态划分方法，分析与我国方法的异同，通过实验进行了验证；通过 Hilbert 变换和小波变换对波浪破碎的相频特性进行了分析，通过建立波高与波压关系来判别破碎波浪力；对破波条件下波浪力的分布进行了统计研究，验证了其分布类型；利用实测资料，分析了不同因素对反射系数的影响，得出了不同周期、波浪要素、破碎率下反射系数体现的不同规律；分析了破碎波作用下削角堤的工作特性，通过对堤面所受的波压力和总力的统计分析，验证了压强分布规律及削角堤对波浪力的削减作用。

The Eca-109 and Eca-109/shRNA cells in 400μM cobalt chloride were cultured for 48h, and the cell cycle was detected by Flowcytometry.

将培养的Eca-109和Eca-109／shRNA细胞在400μM氯化钴缺氧培养48h后用流式细胞仪检测细胞周期，并用Annexin V-PE／7-AAD试剂盒检测细胞凋亡率。

In light of the actual condition in Pangang Group the high efficient compound dephosphorizer is developed by use of the dephosphorizing technology "single slag process" in converter smelting.

结合攀钢实际情况，利用转炉单渣法冶炼脱磷技术，开发应用了高效复合脱磷剂，使平均脱磷率达到89.40%，与转炉预处理双渣法脱磷技术相当，试验炉次的成品w≤0.015%，且未增加冶炼周期，为350km/h高速铁路用钢轨等钢种的稳定生产提供了技术支撑。

Methods Fourteen mutated vpr fragments were selected from patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products were purified, double-cut by Hind Ⅲ and BamH Ⅰ, and the cut products were legated and transformed into competent cells JM109. The 14 reconstructed plasmids were transfected into Hela cells. Cells with pcDNA vpr-wt, pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell were established.

以14个带有HIV-1vpr基因片段的PcD-NA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vpr基因转染细胞、突变株vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经逆转录多聚酶链式反应检测目的基因转染成功后，Pi染色，用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Methods 14 mutanted vpr fragments selected from Chines patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products purified , double-cut by HindⅢ and BamH and the cut products legated and were into competent cells JM109. The 14 reconstructed plasmids electronically transfected into hela cells, and established cells with pcDNA vprwt 、pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell.

将14个带有HIV-1 vpr基因片段的pcDNA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vp r基因转染细胞、突变株 vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经RT-PCR检测目的基因转染成功后，经Pi染色用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Drum sieve using imported technology to produce processing, according to uniform seam screen, opening a high rate, wear-resistant, long life cycle.

筛鼓采用进口技术加工制作，筛缝据均匀，开孔率高，耐磨，使用周期长。

Due to stronger reproductive ability, shorter generation time, smaller movement area, higher cross rates of near relatives, more acaricides contact opportunity, the problem of mite resistance is more dominant than other crop insect pests.

螨类因其繁殖力强，世代周期短，活动范围小，近亲交配率高，受药机会多，其抗药性问题甚至比其他农作物害虫更见突出。

This paper discusses design and realizable methods of remote test output interface from logical design angle.

本文从逻辑设计的角度讨论遥测输出接口的设计及实现方法。

This also 星体投射plies to buildings, structures and geological features.

这也适用于建筑物和地质特征。