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Cell viability was determined by MTT assay, cell cycle and apoptosis rate were analyzed by flow cytometer.

利用MTT法测定细胞成活率,流式细胞仪测试样品的细胞周期与凋亡率。

The results showed that seedlings were abundant in both areas, but survival rates were low. The survival rate curve of the population fell between DeeveyⅠand DeeveyⅡ types. The trends of the disappearance rate and mortality rate curve are consistent. The spectral analysis indicated that there was a periodic regularity in the process of natural regeneration of cercidiphyllum japonicum.

结果表明:两个地区连香树种群幼苗个体比例都比较大,但死亡率很高;存活曲线介于DeeveyⅠ型和DeeveyⅡ型之间;消失率和死亡率曲线变化趋势一致;种群动态过程存在周期性,种群数量动态不仅受基波的影响,而且还显示出了小周期的波动。

(1) Under the estrous cycle of rat is not identified, through supplementary estrogen after surgery, the model can also be successful; Moreover, different doses of estrogen did not affect the rate of success.

(1)在不确定大鼠动情周期的情况下,通过术后补充雌激素,模型也可成功建立;同时过高剂量的雌激素对成模率没有影响。

The established fermentative procedure increases the collection efficiency and expression of recombinant protein.

确定了周期短、产率高且稳定可靠的发酵工艺。

Results Hypericin of different concentrations could significantly inhibit the proliferation and induce the apoptosis of human prostate cancer cells cultured in vitro.

结果:不同浓度的金丝桃素在体外可显著抑制PC3M细胞增殖(P.05),药物浓度为0.5、1.0和2.0 mg·L-1时,抑制率分别为21%、50%和91%;细胞周期出现S期阻滞,由S期进入M2期的比例为零,并诱导PC3M细胞凋亡。

Woodson or apocynum venetum Linn as raw material, it utilizes the method of decoction and alcohol sedimentation to remove impurity. After extract is separated by macroporous adsorptive resins, then use polydextran gel LH-20 to purify, obtain isoquercitrin. Compared with the method of traditional silica gel column chromatography, this method has characteristics such as: experiment period is short, dissipation of organic dissolvent is little, yield of produced product is high, and purity coefficient is fine and so on. Isoquercitrin produced by this invention can be provided for medicine research or used as chemistry control article.

Woodson或罗布麻的叶为原料,采用水提醇沉除杂的方法,提取液经大孔吸附树脂分离后再用葡聚糖凝胶LH-20纯化得罗布麻甲素,此法与传统硅胶柱层析的方法相比,具有实验周期短,有机溶媒消耗少,制得的产品得率高和纯度好的特点,本法制得的罗布麻甲素可供药物研究或化学对照品使用。

The results shows: AM fungi colonization is different in the different Pueraria and changes by the lobata's grow.

调查结果初步表明:不同品种间葛藤根区AM真菌定殖率不同,且随植物的生长周期呈现不同的变化趋势。

Primary cultured human chondrocytes,which were initially obtained from the nasoseptal cartilage, were cultured in vitro and transferred with pEGFP-N1 by means of electroporation wi...

结果 :增强型绿色荧光蛋白基因在转染 2 4h后得到了明显表达,4 8h后流式细胞仪检测其表达率为 35 37%,细胞周期没有明显的改变,且未影响软骨细胞的贴壁过程。

Effects on reproductive performance: The normal delivery of group B,C and D were 80%、93.33%、93.33%,respectively,were improved by a certain extent comparing with the control group; The mean birth weight of calves were not significantly different between the trial and control groups.But numerical datas shown that supplementing Zn-Met in dietary had the tendency to increase the birth weight of calves; Supplementing Zn-Met in dietary can make dairy cows express empathema earlier,and significantly(P.05).Among the trial groups,the advanced extent of the group C was comparatively large(5.8d);The oestrous cycles were all in normal range and not significantly different; The levels of serum PRL of group B,C and D were significantly(P.05) 1.46 ng/ml、3.08 ng/ml、3.45 ng/ml higher than the group A.

对繁殖性能的影响:添加蛋氨酸锌组的正常分娩率为80%、93.33%、93.33%,较对照组(73.33%)有一定程度的改善;试验组犊牛初生重与对照组犊牛初生重差异不显著(P>0.05),但数据结果显示,日粮添加蛋氨酸锌有使犊牛初生重增加的趋势;日粮添加蛋氨酸锌可使奶牛产后发情提前,且差异显著(P<0.05),其中,添加蛋氨酸锌240mg/kg组提前的幅度较大,提前了5.8d;试验组发情周期与对照组差异不显著(P>0.05),均在正常范围之内;B、C、D组血清促乳素含量均比对照组高,且差异显著(P<0.05),依次增加1.46ng/ml、3.08ng/ml、3.45ng/ml,试验组B与C、D两组间也达到显著差异(P<0.05)。

Results The incidence of Pco was 30.77%,39.29%,46.51%,in irregular mestrual cycle group ,oligomenorrhea group ,and amenorrhea group,respectly.

结果 在不规则月经周期、月经稀发组、闭经组中有明显多囊卵巢趋势,多囊卵巢率分别为 30 。77%、39.2 9%、

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