周壁层

By the support of the present project, 3 articles have been published, 4 articles have been submitted to Grana and Acta Botanica Yunnanica etc and some papers is in preparing.

结果表明：蕨类植物孢子外壁和周壁来源于孢子囊内层绒毡层细胞；孢子表面纹饰有的种类由外壁形成，有的种类是由周壁形成；孢子纹饰是在四分孢子形成后迅速形成的，形成的时间很短并比较稳定。

Result: At two weeks after OVA challenge, in model group airway wall thickness and positive cell nucleus of airway smooth muscle begin to grow up. At four weeks after challenge, airway smooth muscle content begins to elevate. At two and four weeks, expression of phenotype mark protein begins to low down, except for sm α-actin at two weeks.

结果发现，模型组大鼠激发二周后出现管壁增厚，四周后出现平滑肌层增厚，二周时即开始出现气道平滑肌Brdu掺入增多；smα-actin于二周时表达增强，而于四周时减弱，sm-MHC、calponin在二周、四周时表达均出现减少；电镜发现平滑肌细胞肌丝稍减少，而高尔基体、粗面内质网等细胞器增多。

The outer perispore layer and the inner perispore layer.

成熟的周壁很厚，可分为外层和内层。

METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.

在差速贴壁+Thy1.1抗体及补体纯化法的基础上，剪开大鼠颅骨，显露位于颅腔前方的嗅球，取出2只嗅球，在显微镜下去除嗅球表面的软脑膜和毛细血管及外周组织，保留富含嗅鞘细胞的嗅神经层和嗅球颗粒层，剪成1 mm3小块分离获取单细胞悬浮液，调整细胞密度至1×107 L-1，接种在用poly-l-lysine包被的培养瓶或培养板中，于37 ℃、体积分数为5%的CO2培养箱中培养，第3天用无血清DMEM/F12培养基换液培养。

PPARγ expression in PAN-treated rats at 12 weeks was present in podocytes and in mesangial cells in segmentally sclerotic glomeruli, with occasional parietal epithelial cell staining.

给予PAN后，PPARγ可以在节段硬化小球的足细胞和系膜细胞上表达，部分在壁层上皮细胞表达。6周后给予吡咯列酮治疗，可以减少系膜区的PPARγ染色，但足细胞上的染色明显增强，伴有小球硬化明显减轻。

A total of 150 mesenchymal neoplasms of the GI tract, abdominal cavity, and retroperitoneum were retrieved from consult and surgical pathology archives, including 54 GISTs (8 KIT-negative; 13 desmin-positive), 17 GI leiomyosarcomas, 11 GI mural leiomyomas, 13 leiomyomas of the muscularis mucosae, 12 gastric schwannomas, 15 inflammatory myofibroblastic tumors, 9 cases of mesenteric desmoid fibromatosis, 10 dedifferentiated liposarcomas, and 9 malignant peripheral nerve sheath tumors.

我们从会诊病例及活检病例中找出150例间叶性肿瘤（来自胃肠道、腹腔和后腹膜），其中包括54例GISTs（8例KIT阴性、13例desmin阳性）、17例GI道平滑肌肉瘤、11例GI道肌壁间平滑肌瘤、13例GI道粘膜肌层平滑肌瘤、12例胃的神经鞘瘤、15例炎性肌纤维母细胞瘤、9例肠系膜韧带样纤维瘤、10例去分化型脂肪肉瘤和9例恶性外周神经鞘膜瘤。

At the fifth passage, two cell strains were made into cell suspension at l×10^9L^(-1). 0.5 mL was subcutaneously incubated at the inguina of each rat for 8 weeks. Amplified cells were cultured in PGC culture medium and non-adherence culture flask till idiosome formed, adhered and differeotiated.

体内实验取连续传5代的2个细胞株，分别制备浓度为l×10^9L^(-1)的细胞悬液，于每只小鼠两侧腹股沟处皮下各接种0.5 mL，培养8周体外分化采用无私附特性的培养瓶和除去白血病抑制因子的原始生殖细胞培养液，以脱饲养层法悬浮培养扩增的细胞，直到拟胚体形成及贴壁分化。

This study was conducted to examiune the fibrotic effect of Ni-Ti and 317L al loys in esophagus.The extract fluid from Ni-Ti,317L alloys was made according t o the ASTM standards of U.S.A. The Fb of esophageal scar was cultured primarily ,then incubated with alloy abstract fluid. The proliferating activity of Fb was measured by MTT at 4, 24, 48, 72 hours in the course of culturing. The esophagu s embedding test of Ni-Ti,317L alloys was made according to ASTM standards of U .S.A.The tissue around the alloys was taken at weeks 2 and 12,and the pathologi c changes were analysed.

为探讨新型支架材料Ni-Ti、317L合金在食管局部的致纤维化作用，按美国ASTM标准制备NiTi、317L合金的金属浸提液；&组织块培养法&原代培养食管壁疤痕的成纤维细胞，传代后以金属浸提液进行培养，分组后分别培养4、24、48、72 h，MTT法检测不同培养时间后Fb增殖功能的变化；按美国ASTM标准进行NiTi、317L合金试件的食管壁内包埋实验，即将金属试件经表面处理后直接置入食管壁粘膜层与肌层之间，术后2、12周取出包埋组织，分析试件周围组织的病理变化，并进行胶原纤维染色，观察纤维形成状况。

Group one consisted of 300 patients whose esophageal stumps were treated by a purse stitch using a clamp. Group two included 180 patients whose esophageal stumps were oversewn by 6-8 interrupted stitches, or by a continuous stitch along the stump before the anvil was in place.

第一组300例，食管残端用荷包钳法作荷包处理；第二组180例，食管残端在荷包钳处理的基础上，再间断全层缝合食管壁并结扎缝线，或沿食管残端连续全层缝合一周，放入抵钉座后结扎缝线。

Injecting in bladder muscle necrotized the bladder wall in situ, but fibroblast generated and took place of the necrotized membrane and muscle four weeks after injection.

膀胱壁肌层注射造成局部坏死，但坏死部位能够在4周内愈合，不会产生大出血、膀胱壁穿孔等严重不良反应。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新