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During 5-week experimental phase, comic strips were used to teach the subjects correlative sentence-writing of successive relation, relation of cause and effect, and adversative relation. And three follow-up sessions were conducted after the first week, the second week, and the third week of the completion of the experimental phase.

在历时五的介入期中,以看漫画写作作为教导受试对象学习连贯、因果与转折关系之关联词造句的方式,而在教学结束一、两及三各进行一次维持效果的测量。

Methods:All of 40 male Sprague-Dawley rats were divided into 3 groups:control group(group A, n = 10), diabetic group for 4 weeks(group B, n = 14), and diabetic group for 12 weeks(group C, n = 16). The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NF-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组:A组为正常对照组(10只),B组为糖尿病4组(14只),C组为糖尿病12组(16只),采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4后,A组、C组大鼠于12后通过注射阿朴吗啡后评价勃起功能,并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达,同时采用电泳迁移率变动分析法进行NF-κB 活性检测。

Methods All of 40 male Sprague-Dawley rats were divided into 3 groups: control group (group A, n=10), diabetic group for 4 weeks (group B, n=14), and diabetic group for 12 weeks (group C, n=16). The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NE-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组:A组为正常对照组(10只),B组为糖尿病4组(14只),C组为糖尿病12组(16只),采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4后,A组、C组大鼠于12后通过注射阿朴吗啡后评价勃起功能,并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达,同时采用电泳迁移率变动分析法进行NF-κB活性检测。

New bone formation was observed to be increasing significamly in both DCN bone cements and DC bone cements with the passage of time. By the end of 24 weeks, new bone had bridged the gap between the proximal and distal fragments in DCN bone cements and DC bone cements. By histomorphological detection in DCN bone cements, Abundant of chondral and new bone islands were seen at the site of defects 4 weeks after the operation and a number of new bones formed and enlarged by both enchondral ossification and appositional formation by the 8th weeks.

研究发现,DCN骨水泥植入4大量间充质细胞分化,在材料与骨端之间出现一层软骨细胞,软骨细胞继续增生并向编织骨分化;8材料降解的同时,小梁骨出现;12新骨长入材料并已经基本替代完成,形成初步骨性连接;24骨缺损完成骨性连接,新骨进行结构改建,材料内部形态结构明显变化。

Group I at 21 and 25 weeks of age and Group Ⅱ only at 25 weeks of age were vaccinated subcutaneously in the nape of the neck with 0 5 ml of MG oil emulsion bacterin respectively.

本试验将30只MG阴性的20龄的蛋种鸡随机分为4组,Ⅰ组于21和25龄、Ⅱ组仅于25龄时分别颈部皮下接种MG油乳剂灭活苗0.5 ml/只,Ⅲ组于攻毒后1接种1 ml/只,另设一组对照,不接种疫苗。

It was found that the low cycle fatigue character has close internal relations with the high cycle fatigue character. In the research of high cycle fatigue of concrete, the test means of cyclic loading between the set constant of force or stress has been extensively used. But this kind of test means can't be used in studying the behavior of concrete low cycle fatigue under large strain.

在研究中发现低疲劳特征与高疲劳特征有着密切的内在联系,但高疲劳问题研究中使用的是在设定的力或应力的上下界限之间重复受力的试验方法,这种方法已无法用于混凝土高应变低疲劳性能的试验研究。

RESULTSdiabetic rats showed typical symptoms involving elevated serum glucose and weight loss (2)The rats in DM+VaD group reacted slower and made more error numbers in Y-maze than the rats in VaD group did, with a prolonged total reacting time in a whole day.(3)More neurons with serious damages, such as edema, ischemia or pyknosis could be found in DM+VaD group than in VaD group. More disarrangement of cone neurons, obvious glia proliferation, and more neuron apoptosis were found in CA area of hippocampus. CONCLUSIONS (1)2-VO in STZ-induced diabetic rats succeeded in establishing VaD animal models.(2) The cognitive dysfunction induced by VaD rats had been deteriorated by diabetes mellitus.(3)Morphology evidences proved that diabetes mellitus aggravated brain damages induced by VaD.PART II Effect of diabetes mellitus on the cholinergic nervous systemin CA1 area of hippocampus of VaDOBJECTIVE To estimate the role of cholinergic nervous system in hippocampus during the process of cognitive dysfunction aggravated by diabetes mellitus in DM+VaD group. METHODS Observe the changes of ChAT protein by immunohistochemistry stain. Chose three rats randomly from each group at each pointat two week, four week, and eight week, then sepa

经腹腔注射链脲佐菌素诱导慢性实验性糖尿病,1后永久性结扎双侧颈总动脉(2-VO)制作VaD模型记录术后2、4和8各组大鼠的体重及血糖;应用Y-迷宫检测空间定向学第二军医大学博士论文中…丈摘…要1ia;一色恤~﹁染澎糕行HE染色观察组织病理学孪叱;胶质细胞原纤维酸性蛋白protellZ,Cf冰尸标记观察星形胶质细舱的激活情况、橄声软公点娜育票票众糯黑橄居端粗默孺篇筑价井袱第二部分糖尿病对血管性痴呆大鼠海马c心区胆碱能神经系统的影响探讨海马CAI区胆碱能神经系统在糖尿病加重确D一大鼠认知障碍中所实验动物及分组同第一部分,应用免疫组织化学染色方法现察海马CAI{介狱牛第晕军l袭拔常博粗{论义

RESULTS:① Gross observation: At 2 weeks, there were no differences between the two groups, and the materials were connected to bone bed with fibrous tissues. At 4 and 8 weeks, there was more new callus in biologic active bone group than in biologic bone group. At 12 weeks, some bionic biphasic ceramic-like biologic active bone were absorbed and new bone regenerated in biologic active bone group, being similar to appearance of host bone, but there were few callus in biologic bone group.

结果:①大体观察:生物活性骨组和生物骨组术后2时无明显差别,材料与骨床间为纤维组织连接。4及8可见生物活性骨组断端围新生骨痂较多,将材料包绕,12时可见材料部分吸收,新生骨长入,修复段塑型较好,基本接近宿主骨外形。12时,生物骨组仅在骨折端有少量骨痂连接。

PART Ⅱ IMMUNOPATHOLOGICAL EVIDANCE OF SIALIC ACID STRUCTURE IN CAMPYLOBACTER JEJUNI AS THE CRITICAL ANTIGEN TO INDUCE PERIPHERAL NEUROPATHY To demonstrate the critical role of NANA structure in the pathogenesis of allergic peripheral neuropathy induced by Campylobacter jejuni and provide immunopathological evidence to confirm the supposition of molecular mimicry and cross-immunity between CJ LPS and gangliosides in nerve.

LPS免疫后第2实验豚鼠的免疫血清中,抗LPS IgG抗体滴度均明显增高,第3达高峰,第5仍维持在峰水平,野生株和突变株LPS免疫血清中的抗-野生株LPS IgG抗体滴度较各自免疫前分别增高8倍和4倍,抗-突变株LPS IgG抗体滴度则较各自免疫前分别增高6.5倍和15倍;(2)全身免疫后第3和第5,野生株LPS免疫血清中检测到较免疫前增高6倍的抗-GM1 IgG抗体,而NANA缺失的突变株LPS免疫血清中一直未检测到抗-GM1 IgG抗体;(3)野生株LPS免疫组中有17.3%的坐骨神经原纤维发生以轴索变性为主(占65%)的免疫性损伤,与突变株LPS免疫组和对照组比较均有显著性差异。

The objective was to determine the effects of active immunization of T-3-BSA in Rom cockerels, 400 cockerels were divided into 4 groups, group 1,2 and 3 were treated with T-3-BSA on different dose(0.2,0.4,0.8mg/mI respectively),within each group,there were 5 treatments following different vaccination schedules, group 4 served as controls. Every injection of immunogen was conducted on different sites around the neck, and the datas were measured at week 11,15,19, combs,wattles,body weight,serum T concentration,carass traits and testicular development and histology were studied in order to find out the most reasonable vaccination schedule and the most possible mechanism in the H-P-G axis of T.

本试验测定了睾酮—3—牛血清白蛋白(T-3-BSA)主动免疫对公鸡性腺与冠垂发育及雄激素水平的影响。400只罗曼蛋用雏公鸡除随机取25只作对照组外,另375只随机分成3大组,每大组125只再随机分成5小组,每小组25只,3大组分别使用0.2mg/ml、0.4mg/ml和0.8mg/ml的T-3-BSA免疫注射,每大组的①②③小组分别于3、6、9龄免疫注射,④小组于3龄首次免疫,6龄加强免疫,⑤小组于3龄首次免疫,9龄加强免疫。

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