周

The communication box was applied in the emotional stress group. All the specimen of TMJ and external pterygoid muscle were taken after 1, 3 and 5 weeks. The microstructural and ultrastructural pathological change in the disc surface, condylar surface and external pterygoid muscle was observed with HE staining, scan electon microscropy and transmission electon microscropy.

本实验采用尚海燕等[3-4]建立的大鼠交流箱心理应激模型，将90只SD大鼠随机分为心理应激组Ⅰ(1周应激组)、心理应激组Ⅱ(3周应激组)、心理应激组Ⅲ(5周应激组)和空白对照组Ⅰ(1周应激对照组)、空白对照组Ⅱ、(3周应激对照组)空白对照组Ⅲ(5周应激对照组)，每组10只；另外30只大鼠仅给予足部电击，作为心理应激组大鼠的应激源，不列入本实验观察对象。

Results］the gross morphology of the tendons showed the experimental group had lesser adhesion and better reactiveness than that in the control group.the fibrin had degraded at 3 weeks. cell tracing showed that the labeled bone marrow derived mesenchymal stem cells remained viable and presented in the intratendinous region for at least 6 weeks, becoming more diffuse at later timepoints.at 3 weeks, collagen fibers appeared more organized and there were better morphometric nuclear parameters in the treatment group. at 6 and 12 weeks, there were no differences between the groups with regard to morphometric nuclear parameters.

结果］实验组相比对照组肌腱大体观察粘连差，肌腱活动性好。3周后纤维蛋白载体即降解，细胞示踪结果显示标记的骨髓间充质干细胞至少6周内仍可保持活性并存在于肌腱组织中，但之后逐渐扩散。3周时，实验组与对照组相比胶原纤维排列更为有序，且胞核形态结构更规则，但在6周及12周时，实验组与对照组胞核参数测定无统计学意义。3周时实验组比对照组具有更强的的生物力学特性，但之后则差别不明显。

Transcript level was not obviously different between MSTN and IGF2. The expression of MSTN reached the highest point at 7 day and droped to the lowest point at 7 weeks after birth. The expression of IGF1 arrived at the highest peak at brith, and degraded the half of the birth after 7 days. Then, the change of the IGF1 expression level was no abvious. The expression result of the IGF2 decreased to the least at 1 week and increased the max point at 3 weeks. The change regulation of IGF2 expression was showed as a wave.

其中，IGF-1表达水平最低，MHC2a表达水平最高；IGF2与MSTN表达水平相当； MSTN的表达于7天时表达水平最高，是出生时表达的3倍，随后于14天时急剧下降，于7周时降至最低水平；出生时IGF1的表达水平最高，随后于一周时降至出生时的一半，后期的表达变化不大； IGF2的表达水平于3周时表达最高，是出生时的两倍，在1周时最低。3周后骤然降低，呈现一个波浪型变化；MHC的表达出生时最高，于1周后降至原来的1/7，随后逐渐升高，但变化并不太大。

RESULTS: FGFR1-positive chondrocytes were abundant in the maturative layer and hypertrophic layer, but only few FGFR1-positive chondrocytes were found in the proliferative layer. In the control group, the expression of FGFR1 increased from 6-week-old to 10-week-old rats, and then decreased and went stable. In both young and adult group, the expression of FGFR1 in the experimental group was significantly lower at 4, 6 weeks and higher at 8 weeks than that in the control group, especially in the young group (P.05). But no difference was found between the experimental group and the control group at 2 weeks in both groups. CONCLUSION: FGFR1 may play an important role in the remodling of condylar cartilage induced by occlusal disorders.

结果：FGFR1主要表达在大鼠髁突软骨过渡层和肥大层，增殖层很少；正常组大鼠髁突软骨从6周龄到10周龄FGFR1表达逐渐增强，10周龄后降低，并保持在一个稳定水平；成年组和幼年组实验4、6周时FGFR1的表达均显著低于对照组，实验8周时的表达高于对照组(P.05)，幼年组尤为明显，实验2周组与对照组无显著差异。

ANP and CX43 began to express at 2nd week after induction and increased gradually,about 60% of the resulting myogenic cells were positive at 4th week after induction ,they were negative for uninduced cells.hMSCs'surface antigen profiles obtained by Flow Cytometry were negative for CD31\CD34\CD45 before and after induction,but CD90 expressed higher after induction while was weak positive before induction(P.05). Apotosis index was correlated with the cultural time after induction,The apoptosis rate of induced hMSCs was remarkably higher than control group(P.01),and the variation between groups was notable(P.05),the cell cycle analysis showed that the percentages of G_0/G_1phases were reduced significantly after induction. The expresstion levels ofβ-MHC and CTNT mRNA were undetectable before induction,began to increase at 1st、4th week after induction,reached the peak at 6th week and decreased after that,the expression of Bcl-2 and Bax mRNA varied regularly after treated with 5-azacytidine. hMSCs'resting membrane potential、range and rate of depolarization were heightened gradually after being induced.

结果：hMSCs诱导前为纺锤形，诱导后第2天部分细胞即开始发生形变，呈球形或短棒状，1周后胞浆中颗粒增多，约20～30%细胞边缘呈毛刷样变化；hMSCs表面抗原CD31、CD34、CD45在诱导前后差异无统计学意义，CD90未诱导时表达呈弱阳性，诱导后明显增高(P.05)；ANP和CX43在诱导前无表达，诱导后第2周开始表达且表达随时间逐渐增强，但CX43在诱导后第5周表达量开始降低。hMSCs诱导后凋亡指数随诱导后培养时间增加，低浓度诱导组低于标准浓度诱导组，组间差异有统计学意义(P.05)，G_0/G_1期细胞比例诱导后较对照组显著减少(P.05)；β-MHC和CTNT基因分别在诱导后第1周和第4周时表达开始增强，在第6周时均达到高峰，第8周时表达开始衰减，Bcl-2、Bax基因表达呈时间依赖性变化，hMSCs经诱导后随心肌样细胞特征的表达膜静息电位、去极化幅值和去极化速率逐渐增高。

Modeling animals: the mortality of 4 weeks old group was 50%,but 6 weeksdidnt die any more; in the 6 weeks group the percentage of CD4. CD8 andthe rate of CD4/CD8 T cell subgroups, the number of lymphocyte inperipheral blood were obviously diminished compared with blank group, hassignificance in statistics(p＜0.01); thymuses and spleens were defl- ated in different degree compared with blank group, has significance instatistics.

结果： 1。模型动物的选择：4周龄组的死亡率为50％，6周龄组未见死亡；6周龄模型鼠一般状况较正常鼠明显变差，且外周血淋巴细胞计数及外周血CD4、CD8百分比、CD4／CD8比值较空白组均明显减少，差异均有统计学意义，P＜0.05或P＜0.01；6周龄模型鼠胸腺、脾脏均不同程度缩小，与正常比较有统计学意义，P＜0.05或P＜0.01。

After repairing skull defect using compound implant, X-ray examination demonstrated that space between peripheral sclerotin and compound implant was covered by high-density shadow after 4 weeks.

复合材料植入免颅骨缺损后，X射线观察术后第4周骨缺损外周骨质与复合材料之间大部分被高密度阴影充填，术后第12周骨缺损外周骨质与复合材料之间完全被高密度阴影充填；光镜观察术后第8周骨小梁大部分相连成片，术后第12周骨小梁粗大，骨髓再生。

Seventy-seven male big rats of four to five months old with the weight of two hundred and eighty to three hundred and twenty grams were divided randomly into contrast group of zero week,contrast group of three weeks,contrast group of seven weeks,contrast group of eleven weeks,experimental group of three weeks,experimental group of seven weeks and experimental group of eleven w...

选用280～320g 4～5月龄wistar雄性大鼠44只，随机分为0周空白对照组11只，3周实验组11只，7周实验组11只，11周实验组11只，0周空白组于0周处死，解剖取大鼠股骨，以生理盐水浸湿的纱布包裹，置-20℃冰箱内保存备用。

All the indexes were tested in 2 -. 4 .. 6 weeks of operative intervals respectively. Results:(1) The general observation in the experimental group ,such as spirit, hair, body weight and activity ,was superior to that in the control group.(2) The recovery rate of sciatic nerve functional index (SF1) was statistically significantly higher in the experimental group than in the control group .(3) electrophysciological indexes: m the experimental group ,the tardy rate of induced motorius action potential ,recovery rates of the amplitude of compound muscle action potential and twitch tension and tetanic tension of triceps surae muscle ,were significantly higher than in the control group.(4) Compared with the control group ,the triceps surae muscle wet weight was significantly greater in the experimental group.(5) histomorphological indexes: the count of myelinated nerve fibers ,diameter of regenerated axon, thickness of nerve myelin sheath and area of capillary were much more in the experimental group than in the control group.(6) observation of ultrastructure with the transmission election microscope: more abundant organell a and maturer myelin sheath lay in the injured peripheral nerve of the experimental group, while not lay in the control group.

分别在木后第2、4、6周时进行各项指标的检测，结果发现：（1）实验组SD大鼠在整个实验过程中，其精神、毛发、体重，运动灵活性等一般情况优于对照组；（2）实验组的坐骨神经功能指数恢复率显著优于对照组，P＜0.01；（3）神经电生理指标：实验组运动神经诱发电位潜伏期的延迟率显著优于对照组，P＜0.05；复合肌肉动作电位振幅的恢复率显著高于对照组，两组比较第2周时P＜0.01，第4、6周时，P＜0.05；小腿三头肌单收缩力和强直收缩力的恢复率实验组显著高于对照组，P＜0.01；（4）小腿三头肌湿重的恢复率，第2、4周时实验组高于对照组，差异非常显著，P＜0.01；第6周时，差异仍显著，P＜0.05；（5）组织形态学指标：有髓种经轴突计数、再生轴突直径的恢复率，毛细血管面积，实验组均显著大于对照组，P＜0.01、P＜0.05、P＜0.001；实验组髓鞘厚度的恢复率也显著高于对照组，第2周时P＜0.01，第4、6周时，P＜0.05；（6）透射电镜超微结构观察：实验组再生轴突中细胞器丰富，髓鞘结构成熟，神经再生情况优于对照纽。

It is concluded that the expression of hERMAP in fetal liver approximately consistent with haematopoiesis of fetal liver, and the expression of this gene in bone marrow aged 15-32th fetal weeks coincides with haematopoiesis of fetal bone marrow.

FQ-PCR结果显示，人类ERMAP基因在9-36周胎儿肝脏中均有表达，其表达量从第12周开始升高，18-20周达高峰，21周后逐渐下降；该基因在胎儿骨髓中的表达则从15周开始，27-32周达高峰，之后迅速下降，其表达量低于肝脏；在其余7个脏器中仅有部分标本呈现低水平表达。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。