启动

The different of the two plasmid lied in different Promotors ,since strong paomotor may paly a important role in gene expression in Virat vector .

他们的区别在于含有不同的启动子，因为强启动子可能在病毒载体系统的基因表达上起关键的作用。

Subtilis chromosomal DNA. To the best of our Knowledge, this report is the first time to clone and express promotors from BCG in B.subtilis, and proved that the signal of transcription from BCG can be recognized by B.

就我们所知，我们的实验是首次将卡介苗的启动子在枯草杆菌进行克隆和表达，证明了卡介苗的启动信号也能为枯草杆菌所识别。

It activates many virus and mammalian cells'promotors through the binding with GC/GT box, deciding the efficiency and specificity of the transcription of promotor. Sp3 gene turning out both activating and repressing function, even to the same gene, it can also develop the different function in different period, and therefore has got the important meaning to numerous genes of human body.

Sp3（Specificity protein，Sp3）基因是一种核转录因子，属Sp家族成员之一，是继Sp1后发现的具有与之相同的DNA结合区的转录调节因子，近年来发现它对众多基因均具有调节作用，它通过与GC／GT box结合，激活许多病毒和哺乳动物细胞的启动子，决定启动子的转录效率和特异性。

The BDDcFⅧ gene was ligated behind PUB and 20H1 promotors to create lentiviral vectors pTK161 and pTK162. Meantime lentiviral vectors pTK161' and pTK162' were produced by cloning a green fluorescent protein into pTK151 and pTK152, which was driven by PUB and 2OH1 promotors respectively. Vector supernatant were prepared by using transfer calcium phosphate mediated-cotransfection of 293T cells. The virus vector,△NRF packaging-plasmid, and VSV-G envelope-plasmid was assayed by titers and cFⅧ activity in cell culture supernatant after infection into 293T cells.

用构建携带cFⅧ基因的慢病毒载体pTK161和pTK162（含20HI启动子），同时构建含绿色荧光蛋白的慢病毒载体pTK161'和pTK162'（含2OH1启动子）的方法，分别与包装质粒△NRF、包膜蛋白质粒VSV-G共转染293T包装细胞，将包装好的病毒颗粒再感染293T细胞，检测病毒滴度和培养细胞上清中cFⅧ活性。

Disable or remove any newly installed software.if you need to use safe mode to remove or disable components,restart your computer,prees F8 to select Advanced startup options,and then select safe mode. Technical information:*** STOP:0

禁用或删除任何新安装software.if你需要使用安全模式，以消除或禁用组件，重新启动电脑， prees F8将评选先进的启动选项，然后选择安全模式。

These results suggested that the function of each function domain in HIV 1 LTR exhibited distinct characteristics on gag gene expression in vaccinia virus:(1) The function of intact LTR on gag gene expression varied with the upstream poxvirus promoter;(2) NR sequence neither decreased nor increased the Gag protein exprssion level;(3) Enhancer sequence might not be recognized by recombinant vaccinia virus expression system;(4) TAR sequence enhanced Gag protein expression effectively;(5) U5 region and its downstream non translated sequence had little effect on

免疫印迹和免疫酶试验检测均表明，6株重组病毒的Gag蛋白表达量因LTR不同而有明显差异，表明HIV1的LTR及其下游基因置于痘病毒启动子控制下，在痘苗病毒中表达时有下述特点：（1）不同的痘苗病毒启动子与全长LTR相互作用，对gag基因表达有显著不同的调控效果；（2）NR序列对Gag蛋白表达没有明显影响；（3）EN序列不能被重组痘苗病毒表达系统识别；（4）TAR序列可提高Gag蛋白的表达量；（5）U5区及下游非翻译序列不影响Gag蛋白的表达。

For the application of Balance of heat loss with limit high ,we can choose low a balanced current, high voltage start a balanced, low accuracy, as well as reduce the number of the battery in series to reduce heat loss of balance; In the situation that heat capacity of the battery pack requirements is easy , You can choose a higher balance of the current, low voltage start a balanced, high precision balance, as well as more battery in series to increase the number of battery-balanced speed.

对均衡热损耗限制高的应用，可选择低均衡电流、较高的均衡启动电压、较低的均衡精度以及较少的同时均衡电池数来减低均衡热损耗；而在电池包热容量宽裕的情况下，可以选择较高的均衡电流、较低的均衡启动电压、较高的均衡精度以及较多的同时均衡电池数来提高均衡的速度。

The oil pump control system of governor oil pressure unit at power plant of Naban reservoir was modified with contact-free,soft start,intelligent pressure control unit,which not only remove the defects from traditional oil pump motor control mode consisting of relay and contactor,but also upgrade the control automation degree,improve starting performance and permit convenient operation.

通过利用无触点的智能软启动压力控制装置对那板水库电站调速器油压装置油泵控制系统的改造，解决了油泵电动机采用传统继电器———接触器控制方式所存在诸多缺陷，提高控制的自动化程度，改善启动性能，方便操控，经过3年多的应用，运行良好，为水电站的调速器油压装置油泵控制提供了一个好的借鉴。

Consequently,in this study we introduced genes that encode the xylose isomerase and xylulokinase precisely under the control of a strong,constitutive glyceraldehydes-3-phosphate promoter by PCR-mediated overlap extension,which is in charge of xylose assimilation;and also we introduced genes that encode the transaldolase and transketolase precisely under the control of enolase promoter,which is in charge of xylose utilization,then transformed plasmid with the two operons into Z.

因此，本文通过代谢工程手段，将负责木糖代谢转化的木糖异构酶基因和木酮糖激酶基因置于强组成型启动子3-P-甘油醛下；同时将负责木糖转化利用的转醛醇酶和转酮醇酶基因置于强组成型启动子烯醇酶下，通过电转的方式将上述四个基因转入到Zymomonasmobilis CP4中，从而在运动发酵单胞菌中构建起一套完整的木糖代谢途径。

Result (1) The promoter of hTERT and Cox-2 was subcloned.(2) The hTERT and Cox-2 promoter controlled oncolytic adenovirus was gained.

结果 成功构建了hTERT和Cox-2启动子，并将两个启动子连接入腺病毒载体，产生了具有感染力的可增殖腺病毒。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。