启动

The inhibitor for DNA methyltransferase, 5-azacytidine (5-azaC), can demethylate the methylated DNA and restore transcription of genes. High dose homocystine can mediat DNA methylation and suppress gene expression.

应用甲基转移酶抑制剂5-氮杂胞苷(5-azacytidine， 5-azaC)可以使甲基化的启动子去甲基化，启动基因重新转录；高浓度同型半胱氨酸(homocystine， Hcy)可以介导基因甲基化而抑制其表达。

In this paper, an escherichia coli-saccharopolyspora erythraea shuttle vector containing the erya promoter region was constructed using the enhanced green fluorescent protein gene as a reporter. the shuttle plasmid was transformed into sac.erythraea a226 and streptomyces lividans jt46 by peg mediation, respectively. fluorescence microscopy confirmed that the egfp was expressed in both strains.

本文克隆了erya基因的启动子perya，以绿色荧光蛋白基因为报告基因，构建了大肠埃希菌-糖多孢红霉菌穿梭型质粒。peg介导原生质体转化法将穿梭型质粒分别转入糖多孢红霉菌a226与变铅青链霉菌jt46，荧光显微镜检测发现，此启动子在两菌株中都具有功能。

However, the mutation of GATA site at -2948bp remarkably reduced the reporter gene activity driven by SV40 promoter, but not by ε-globin gene proximal promoter. To test whether those sites act synergistically, we mutated 2 of these three binding sites with different combinations.

然而，GATAb（在-2948bp的GATA位点）的突变明显地降低了SV40启动子介导的报告基因转录活性，但是对ε-珠蛋白基因启动子介导的报告基因表达却影响不大。

The result indicates the length of the clone sequence is 963bp and shares a similarity of 45.24 % with the published Glycine max glycinin subunit G7 (Gy7) gene promoter.but have more similar to quantity and distance of promoter elements, they all contained typical TATA-box, CAAT-box and necessary regulatory motifs of seed-specific expression.

测序结果表明所克隆的片段长为963bp，与预期设计的大小一致，该片段与已发表的11S球蛋白基因启动子序列的相似性为45.2％，但所含的启动子序列元件的数量和距离上很相似，均具有有典型的TATA盒和CAAT盒，以及种子特异表达所必须的调控元件。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

The promoter of mitochondria-localized small heat shock protein gene in Lycopersicon esculentum (Lehsp23.8) is characterized as strongly heat-inducible.

番茄线粒体小分子热激蛋白(Lehsp23.8)启动子是典型的热诱导启动子。

a mandatory testing device to prevent drunk driving is proposed.it can mandatorily test whether and how much a driver or a sailor on watch drunk.only when the alcoholicity of the exhalation breathed out by the people tested is normal,small type or automobiles can startup,the person on watch of a large ship can register as working.otherwise,if the alcoholicity is overproof,small type ships or automobiles can't startup,and neither does the device allow the person on watch register as working and it can inform the captain via alarm.in this way,drunk driving problem can be solved effectively only by setting a simple device.

该装置具有强制检测机动车驾驶员和船舶值班人员饮酒状态的功能，当被测人员呼出的气体酒精含量正常时，机动车或者小型船舶可以启动，大型船舶的值班人员可以记录为值班；当被测人员呼出的气体酒精含量超标时，机动车或者小型船舶无法启动，大型船舶的值班人员则不能记录为值班，同时可以通过报警器通知船长。这样，仅通过安装使用这样一个简单的装置，就有效地解决了酒后驾驶机动车和船员酒后值班的问题。

PPARα ligands suppressed OPN promoter actiity, and an actiator protein-1 consensus site conferred this repression. Oerexpression of c-Fos and c-Jun reersed the inhibitory effect of PPARα ligands on OPN transcription, and, in chromatin immunoprecipitation assays, PPARα ligands inhibited c-Fos and phospho–c-Jun binding to the OPN promoter.

PPARα配体可抑制OPN启动子活性，一个激活蛋白-1的一致序列位点与这种抑制有关。c-Fos和c-Jun的过表达可逆转 PPARα配体对OPN转录的抑制作用，并且在免疫沉淀实验中，PPARα配体可抑制c-Fos及磷酸化c-Jun与OPN启动子的结合。

The ice/liquid mixtures of the coolant and the sample solution were respectively added in the Dewar bottle and the sample container to precool for 2~3 hours under stirring. Then some of the coolant liquid was replaced with coolant ice, the motor stirrer was restarted, and the temperatures were read from the Beckmann thermometer.

实验时，先调节寒剂的组成使其凝固点低于待测溶液的凝固点～2℃，然后分别在保温瓶和凝固点杯中加入寒剂和待测溶液的冰水，启动搅拌器预冷却2~3小时，接着更换保温瓶中的寒剂冰水，重新启动搅拌器，记录贝克曼温度计读数。

A 1. 13kb part of the BCR promotor region was amplified and sequenced from 30 CML patients and 19 controls.

BCR基因启动子区用PCR方法扩增了1.13kb的序列范围，通过测序的方法获得了30例CML和19例对照的启动子区序列。

Shop assistant: Please check at the door.

店员：请您到门口柜台交费。

In the end, transportation of PAHs in the environmental matrix was preliminarily investigated and discussed according to PAHs data of soils and sediments from a certain region in Shanghai. The result indicated that there are two or more pathways on PAHs transportation in the environmental matrix.

最后，对上海特定地区内PAHs在环境介质中的迁移径途进行了分析讨论，结果表明上海市土壤和沉积物等环境介质中PAHs的迁移存在多种途径。