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听毛细胞

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Results①Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear;②The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Cortis tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.③Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins,neurotrophic factors and neuron special proteins.

结果①HE常规染色大体观上了解了C57BL/6小鼠内耳形态发育E8-Da期间的一般规律并且获得了E13的三维立体图像;②获得了各种听功能密切相关的结构的成熟时间点,基底膜(D10)、鼓阶前庭阶(D4)、螺旋器隧道(D12)、内螺旋沟(D10)、盖膜(D10)、毛细胞(D12)、螺旋神经元(D12)和蜗轴纤维(D12-D14);③完成了前神经决定蛋白、神经分化蛋白、神经营养因子和神经元特异性蛋白等内耳发育相关的蛋白的表达时限表并详细观察了其在内耳各个部位表达随发育变化的特点:④Calbindin在E16以后的螺旋神经元没有表达,但是在scarpa神经元持续表达。

Results①Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear;②The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Corti\'s tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.③Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins,neurotrophic factors and neuron special proteins.

结果①HE常规染色大体观上了解了C57BL/6小鼠内耳形态发育E8-Da期间的一般规律并且获得了E13的三维立体图像;②获得了各种听功能密切相关的结构的成熟时间点,基底膜(D10)、鼓阶前庭阶(D4)、螺旋器隧道(D12)、内螺旋沟(D10)、盖膜(D10)、毛细胞(D12)、螺旋神经元(D12)和蜗轴纤维(D12-D14);③完成了前神经决定蛋白、神经分化蛋白、神经营养因子和神经元特异性蛋白等内耳发育相关的蛋白的表达时限表并详细观察了其在内耳各个部位表达随发育变化的特点:④Calbindin在E16以后的螺旋神经元没有表达,但是在scarpa神经元持续表达。

Neurotrophic factors is a kind of chemistry which can increase the survival rate of neurone and the growth of nerval spines, it is important in neurone development, neurite growth, transmitter synthesis and cell languish.

神经营养因子又具有促进听觉神经系统发育并维持其正常功能的作用,其作用贯穿于从听板形成直至Corti器毛细胞与传入、传出神经末梢纤维之间建立突触关系的全过程。

Methods Fifty F-344 rats were divided into four groups, GM only、PAA+GM、PAA only and saline control. ABR thresholds at different frequencies were measured at different times and hair cell losses were numerated. Two-dimensional diffusion assays in the culture medium were performed to evaluate the effect of PAA on the antimicrobial activity.

选用健康F-344大鼠50只,随机分4组:Ⅰ为GM、Ⅱ为PAA+GM、Ⅲ为PAA、Ⅳ为生理盐水对照组;通过观测4组大鼠不同时期、不同频率听性脑干反应(auditory brainstem respons,ABR)阈值的改变;计数耳蜗毛细胞死亡率,以观察PAA对F-344大鼠GM耳蜗毒性的拮抗作用;用双向扩散血清培养基检测法观察PAA 对 GM 抗菌活性的影响。

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