听毛细胞

Objective: To modify the technique of surface preparation of cochlea and staining for hair cell examination.

目的 ：探讨一种观察耳蜗听毛细胞病理改变的耳蜗铺片与酶组织化学检查技术。

Methods Twenty guinea pigs with gentamicin-induced hearing loss were randomized equally into IGF-1 group and control group. In both groups, scala tympani fenestration was performed for injection of IGF-l (10μ1) or artificial perilymphatic fluid (10μl). Auditory brainstem responses test was performed before and 7 and 14 days after surgery, respectively, and the cochlea was removed by decollation of 3 guinea pigs from each group after ABR test for observing the changes in the hair cells using scanning electron microscope.

豚鼠20只，施用庆大霉素致耳聋后均分为IGF-1组和对照组，行鼓阶开窗术，微孔技术注入试剂（IGF-1组注入鼠重组IGF-1，对照组注入人工生理外淋巴液）10μ1，分别在术前和术后7、14d行ABR测试；测试完后每组3只断头取听泡，在扫描电镜下观察耳蜗毛细胞改变。

They discovered that it does not take much of a push on the stereocilia to get the hair cell to respond.

他们发现，只要轻轻一碰静纤毛，听毛细胞就会作出反应。

The results suggest that cochlear outer hair cells are normal in function with dysfunction of the cochlear nuclei. The main lesion of the disease are neuropath related to stepedius reflex and medial olivo-cochlear efferent nervous system.

此类原因不明的低频听力减退患者听力学表现提示：耳蜗外毛细胞功能正常，与声反射、听传出抑制、ABR波III相关的脑干听觉神经径路为功能障碍的主要部位，可伴有耳蜗或传入神经功能障碍。

In natural culture condition in vitro, otocyst cells of fetal rats can differentiate or proliferate to immature hair cells.

胎鼠听囊细胞在体外自然培养条件下可以分化或者增殖为未成熟毛细胞。

CONCLUSION摘要: In natural culture condition in vitro, otocyst cells of fetal rats can differentiate or proliferate to immature hair cells.

结论摘要：胎鼠听囊细胞在体外自然培养条件下可以分化或者增殖为未成熟毛细胞。

Month culture. CONCLUSION: In natural culture condition in vitro, otocyst cells of fetal rats can differentiate or proliferate to immature hair cells.

胎鼠听囊细胞在体外自然培养条件下可以分化或者增殖为未成熟毛细胞。

Then the animals were sacrificed and the surface preparation and the section of cochleae stained by MyosinⅥwere observed.Other cochlea were observed with SEM.

分别于术后第3.5个月分别行听性脑干反应检查后取双侧耳蜗标本做基底膜铺片和耳蜗冰冻切片MyosinⅥ染色观察有无新生毛细胞样细胞出现，部分耳蜗作扫描电镜观察。

The experiment suggested that hair cell and its nerve ending as well as marginal cell of SV are the sites liable to be damaged by the ototoxic affect of KA.

实验提示：听毛细胞及其底部的神经末梢、血管纹边缘细胞是卡那霉素耳毒作用的主要部位。

The present study is designed to seek the evidences of regeneration of auditory epithelia and acoustic nerve in the postnatal chick, By using the methods of tissue cultures, scanning electron microscopy, autoradiography and the cell image analysis technique, we observed the regeneration of auditory hair cell after intense sound exposure, and also neuron repair following a mechanical injury with either in vivo or in vitro.

本研究应用物理性噪声，建立了内耳听器-基底乳头声损伤的模型和初级听神经元纤维机械损伤的模型。在此基础上应用体外组织细胞培养、扫描电镜、放射自显影、光镜以及细胞图象分析等多种形态学方法，综合分析了听觉毛细胞和初级神经元轴突的再生反应。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了