听囊

Otocyst cells ; stem cells ; cell proliferation ; differentiation ; fetal rats

听囊细胞；干细胞；细胞增殖；细胞分化；胎鼠

Keywords otocyst cells; stem cells; cell proliferation; differentiation; fetal rats

听囊细胞；干细胞；细胞增殖；细胞分化；胎鼠

In natural culture condition in vitro, otocyst cells of fetal rats can differentiate or proliferate to immature hair cells.

胎鼠听囊细胞在体外自然培养条件下可以分化或者增殖为未成熟毛细胞。

CONCLUSION摘要: In natural culture condition in vitro, otocyst cells of fetal rats can differentiate or proliferate to immature hair cells.

结论摘要：胎鼠听囊细胞在体外自然培养条件下可以分化或者增殖为未成熟毛细胞。

Month culture. CONCLUSION: In natural culture condition in vitro, otocyst cells of fetal rats can differentiate or proliferate to immature hair cells.

胎鼠听囊细胞在体外自然培养条件下可以分化或者增殖为未成熟毛细胞。

The present study is to culture otocyst cells in fetal, cochlear sensory epithelial cells of neonatal and mature respectively in vitro with refinement of culture media and techniques. Immunocytochemistry and Bromodeoxyuridine labeling are used to detect properties and mitotic status of cultured cells. With the observations about proliferation and differentiation in cultured cells, we want to find the origin of regenerative cells and to understand how these progenitors proliferate and differentiate.

本研究分别对胎鼠听囊、新生鼠听觉上皮和成年鼠听觉上皮用自然培养方法进行体外培养，并应用免疫细胞化学方法，对培养细胞进行染色标记和观察，系统了解哺乳动物听觉细胞发生、发育过程，以及这些细胞在体外的增殖和分化过程，旨在了解哺乳动物听觉细胞听觉上皮感觉细胞的来源，发现细胞生长的前体细胞。

METHODS: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells.

取孕鼠胚胎听囊细胞进行体外培养，用免疫细胞化学方法，分别对培养的细胞进行细胞角蛋白、F肌动蛋白和calretinin染色观察细胞特征；BrdU染色观察细胞增殖。

METHODS摘要: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells.

方法摘要：取孕鼠胚胎听囊细胞进行体外培养，用免疫细胞化学方法，分别对培养的细胞进行细胞角蛋白、F肌动蛋白和calretinin染色观察细胞特征；BrdU染色观察细胞增殖。

In 4 cases, the cavities were full of perilymph. We could find that the cavity was irregularly round-shaped, about 6.5 mm in diameter, with thin mucosa adhere to the cavity wall. In the center area, around the internal acoustic meatus fundus, there was a horseshoe- shaped cystic structure about 3 mm3, which was a combined structure of the saccule, utricle and scala media. In 1 case, there was little perilymph in the cavity, with about 1.5 mm thick soft tissue and granulation adhere to the wall. The saccule, utricle and scala media were not recognizable.

结果 对5例患者进行共同腔内耳蜗内镜检查，见4例腔内充满液体，吸出后腔壁有扉薄黏膜附着，腔呈不规则圆形，直径平均约为6.5 mm，中央区贴近内听道底部位见马蹄形或肾形囊腔，约 3 mm3大小，为内耳球囊、椭圆囊和中阶畸形融合的结构；1例腔内淋巴液较少，共同腔壁附着软组织和肉芽，约1.5 mm厚，无法将腔壁上的软组织与中阶、球囊、椭圆囊的结构区分。

Results Five cases with common cavity underwent micro-endoscopy during cochlear implantation. In 4 cases, the cavities were full of perilymph. We could find that the cavity was irregularly round-shaped, about 6.5 mm in diameter, with thin mucosa adhere to the cavity wall. In the center area, around the internal acoustic meatus fundus, there was a horseshoe- shaped cystic structure about 3 mm3, which was a combined structure of the saccule, utricle and scala media.

结果 对5例患者进行共同腔内耳蜗内镜检查，见4例腔内充满液体，吸出后腔壁有扉薄黏膜附着，腔呈不规则圆形，直径平均约为6.5 mm，中央区贴近内听道底部位见马蹄形或肾形囊腔，约 3 mm3大小，为内耳球囊、椭圆囊和中阶畸形融合的结构；1例腔内淋巴液较少，共同腔壁附着软组织和肉芽，约1.5 mm厚，无法将腔壁上的软组织与中阶、球囊、椭圆囊的结构区分。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。