含量

The protein and phospholipid constituent in membrane of neurocyte in brain of chicken with encephalomalacia was separated and studied with the method of SDS-PAGE electrophoresis and the method of high performance-thin layer chromatography respectively The fluidity of neurocyte membrane of chicken with encephalomalacia was determined for the first time The content of Ca and trace elements were determined in chicken encephalomalacia systematically and the status of Fe ion in cerebellum was observed. The activity of enzymes that were relative to metabolism of energy and neuro-medium in brain were checked systematically with the method of enzyme-histochemistry. Pathological examination was carried out by microscopic and ultramicroscopic observation. The apoptosis of neurocyte in brain was checked.

在进行临床观察、定期剖杀并检测体内V〓含量、体重和脑重的基础上，重点研究了与神经损伤有关的以下几个方面的内容：系统地检测了体内自由基代谢和抗氧化系统功能的变化；首次采用SDS-PAGE电泳法分离并检测了雏鸡脑软化症神经细胞膜蛋白组分，并以高效薄层层析法检测神经细胞膜磷脂组分；测定了脑软化症雏鸡神经细胞膜的膜流动性；首次系统检测了脑软化症雏鸡体内钙及微量元素含量，并进行了小脑组织总铁离子状态观察；采用酶组织化学方法系统地观察了脑组织中与脑能量代谢和神经递质有关的酶的活性；对相关组织进行了病理组织学和超微结构观察；并对脑组织中神经细胞的凋亡进行了检测。

The results showed that LRK could significantly inhibit the mammary hyperplasia caused by estrin, reduce the content of serum 〓, raise P level, promote the production of DA in hypothalamus and mammary tissue, decrease the release of 5-HT, inhibit the secretion of serum PRL, reduce the sensitivity of mammary tissue to estrin and progestogen, prevent the expression of mammary tissue on PCNA, the effect was equal to triphenylamine oxide, obviously superior to the control group of Ru Zeng Ning.

通过实验研究，建立了具有肾虚肝郁证侯群的乳腺增生病理模型，利用免疫组化技术、图像分析系统、荧光分光光度法、放射免疫法等现代医学研究方法，从病理形态学、医用体视学、神经内分泌学、阴道细胞学等方面，观察了利乳康口服液对模型动物整体状态、乳腺组织病理形态、血清激素、神经递质以及卵巢功能的影响，结果表明利乳康口服液能明显抑制雌激素引起的乳腺组织增生，降低血清E〓含量，升高P水平，促进下丘脑及乳腺组织中DA的产生，减少5-HT释放，抑制血清PRL分泌，降低乳腺组织对雌、孕激素的敏感性，阻止乳腺组织PCNA的表达，其效应与三苯氧胺相当，明显优于乳增宁对照组；另外，利乳康口服液还能缓解模型动物肾虚肝郁证侯群，增加其体重，调节其整体状况，改善模型动物不规则动情周期，减轻其子宫及卵巢的病变，抑制子宫内膜的过度增长，调节卵巢功能，揭示了中药利乳康口服液对神经内分泌和卵巢功能的整体调控作用，以及对本病发生、发展的预防作用

The release in vitro of insulin from Ins-NC can be described by double exponential equation.

研究表明注射用胰岛素纳米囊具良好的外观及再分散性，冷冻干燥前后平均粒径及包封率几乎无变化，初步稳定性实验表明，注射用胰岛素纳米囊在4℃及室温较稳定，含量，包封率，平均粒径等无变化，体外释药符合双指数模型。

Methods On the basis of bilirubin induction and NMDA receptor an-tagonist GAPA administration in guinea pigs. The brain extracellular EAA neurotransmitter was ex-tracted with microdialysis and analysed with HPLC; then, brain tissue ATP was measured by fluores-cence assay and intracellular Ca2 + was measured by fluorescence imaging analysis.

制作胆红素脑病动物模型基础上，在体脑内微透析提取兴奋性氨基酸神经递质，HPLC检测天冬氨酸、谷氨酸、甘氨酸，并给予模型动物NMDA受体拮抗剂GAPA干预，电镜检测脑组织超微结构，荧光检测脑组织ATP与细胞内Ca2+含量。

Acid dye colorimetry ; compound belladonna mixture ; hyoscyamine ; content

酸性染料比色法；复方颠茄合剂；莨菪碱；含量

Results showed that L-THP possessed negative inotropic and negative chronotropic action, lowed left ventricular pressure and it's infinitesimal calculus, decreased heart output in per min., shorten 〓 and 〓 of action potential of ventricular papillary muscle of guinea pig and even the plate-phase of action potential disappeared in larger dose group , but prolonged 〓. we studied transmembrane transportation of 〓 after administering L-THP , L-THP not only depressed uptake and increased release of Ca 2+ in normal myocardium, but also it showed stronger inhibition in cell injured by ISO, and fully eliminated the effect of ISO increasing uptake and decreasing release of 〓. By detecting the 〓 content of ventricular single myocyte using fluorescent probe Fluo-3/AM, results correspond to that done above, L-THP decreased 〓 content inside of cell significantly. Using whole-cell mode, we observed the effect of L-THP on Ica in ventricular single cell, it was found that L-THP decreased Ica, the effect increased with increasing it's dose, and had voltage, frequency-dependency. Dose highly corelated to effect, corelative constant is 0.87. When washing cell with normal tyre's solution, Ica could recover, which proved the blocked action of L-THP on calcium channel once again. Meanwhile, L-THP reduced production of MDA in heart injured by ISO in dose-dependent manner, improved content of GSH-Px, in case tissue or cell free from free radical damage.

结果显示，该药物具有负性肌力和负性频率作用，降低左室压及其微分，终使每分心输出量减少；缩短豚鼠心室乳头肌动作电位时程的〓和APD〓，大剂量使动作电位平台消失，〓延长；同位素示踪法研究证实L-THP抑制〓摄取，促进〓释放，降低细胞内的〓含量；单通道水平的研究证实，L-THP减少豚鼠心室肌单细胞内向钙电流，且具有剂量，电压，频率依赖性，其作用易于洗脱，药物剂量与Ica也具有高度相关性（相关系数r为-0.87）；L-THP还可抑制ISO损伤心肌组织所致的丙二醛含量增加，增加谷胱甘肽过氧化物酶的量，使组织细胞免受损伤。L-THP阻断L-型〓通道，抑制〓的摄取，促进〓的释放，降低细胞内〓含量L-THP作用的离子基础。

The experimental result shows:1 acupuncture and moxibustion on DU4 and RN4 can lighten inflammationreact , suppress the inflammation course, DU4 has the best result.2 IL-2 is the important factor of participating the immune respondence.Acupuncture and moxibustion can increase IL-2 content in the blood , thusstrengthen the immune function of the organism .3 acupuncture end moxibustion can raise β-EP content of lower thalamus ,hypophysis , lymph gland. it proves that acupuncture and moxibustion can stimulateand accelerate compound、store 、process 、shifting utility of β-EP content; andstrengthen the immune regulating function of the organism, acupuncture function isstrong rapidly, moxibustion function is slow and gentle, the regulating action powerof moxibustion has close relations with choice of the point . The possible reason isthat y whether acupuncture and moxibustion bring into play their regulating actionthrough different way of neural body fluid.4 The DU4、RN4、FEI point influence rule apply on change of β-EP content ofabove-mentioned organism.

实验结果显示：1 针灸命门和关元穴能减轻炎症反应，抑制炎症过程，命门穴效果最好。2IL-2 是参与免疫应答的重要因子，而针灸能增加血中 IL-2 含量，从而增强机体的免疫功能。3 针刺和艾灸可以升高下丘脑、垂体、淋巴结β-内啡肽含量，说明针灸可以刺激并加速β-内啡肽含量合成、储存加工和转移利用；增强机体的免疫调节功能，针刺作用迅速强烈，艾灸作用缓慢温和，艾灸的调节作用强弱还与穴位的选择有密切关系。

Results showed t hat TFM (400,40 mg/kg/d) markedly reduced HW/BW, LVW/BW, left ventricular Ca 2+content and myofibril diameter, but without effect on systolic blood pressur e.

结果：水杉总黄酮(400， 40 mg/kg.d)能显著降低 hW/BW、LVW/ BW、心肌纤维直径、左心室肌 Ca2＋含量，对大鼠收缩压无显著性影响。

Experiments were performed in three groups of mice,20 minutes later ,mice in control group were treated with 2ml PBS by ultrasonic nebulization for 20 minutes, mice in treatment group were treated with rhIL-10(10ng) by ultrasonic nebulization for 20 minutes.

结果：经DNA测序证实，克隆的hIL-10cDNA与文献报道的一致，应用ELISA方法检测证明在大肠杆菌中成功表达出了hIL-10，含量＞400pg/ml，检测其生物学活性与标准品无明显差异。

Soil ; Natural Radioactivity ; Nuclide ; Content ; Investigation ; China

土壤；天然放射性；核素；含量；调查；中国

The circulation, consumption and reproduction of the trilogy have not only testified to its historical significance and literariness, but also invested it with new meanings.

该三部曲的流通、消费与再生产不仅验证了其历史意义及文学性，而切还赋予它以新的意义。

If he thought that he could buy me off, he would be wrong.

如果他认为他可以收买我，那他就大错特错了。