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Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库,在大肠杆菌中扩增后提取混合质粒DNA,经电转化非缺陷标志酒精酵母 AS.2.1364,在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子,从阳性转化子中分离重组质粒证实含5.0kb的插入片段,用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

Being better than high quality edible alcohol and produced by mordern scientific technologies, high pure alcohol, with specific properties of its smell, physical and chemical quality index, and content of fusel oil,aldehyde group and ester group, is widely used in liquor-making industry.

摘 要:超过优级的食用酒精称之为高纯度酒精,对其气味、含杂醇油、醛类和酯类等感官和理化质量指标都有特殊要求,其生产制作需要现代科学手段。

Some of the most popular items at the Market are the Nativity Scene (also known as a crèche or crib); Zwetschgamnnla (figures made of decorated dried plums); Nussknacker; Gebrannte Mandeln or candied, toasted almonds; assorted cookies like Lebkuchen and Magenbrot; Christstollen or Stollen, a sort of egg bread with candied fruit; and not least, Glühwein, hot mulled wine (with or without a shot of brandy), or Eierpunsch (an egg based warm alcoholic drink), to keep the body going in the cold winter air.

一些最著名的市场的要素是耶稣降临场景(或作crèche或crib),Zwetschgamnnla(用装饰过的干李子做成的人物),Nussknacker,Gebrannte Mandeln,分类饼乾例如蜂蜜饼或者开胃饼,Christstollen或Stollen以及必不可少的烫红酒,或者 Eierpunsch(一种鸡蛋为主的温热含酒精饮料),这些在严冬寒冷氛围下暖肚。

The alcohol is then aerated, which causes it to convert, through the action of Acetobacter bacteria, to acetic acid, water, and various other compounds.

原料中必含糖,酵母发酵后生成酒精,酒精受醋酸杆菌的作用,即生成醋酸、水和各种其它化合物。

The alcohol is the n aerated, which causes it to convert, through the action of Acetobacter Bacteria, to acetic acid, water, and various other compounds.

原料中必含糖,酵母发酵后生成酒精,酒精受醋酸杆菌的作用,即生成醋酸、水和各种其它化合物。

Sour,hot,pungent,bitter, salty,mustard,chillies,onion,alcoholic liquors,acidic things,stale food,overripe or unriped food and fruits and other articles that disagree with your system should be avoided.

禁忌食物:过酸,过辣,过苦和刺激性强的食物,过咸的菜肴,含芥末的,辣椒,洋葱,酒精饮料,酸性食物,不新鲜的,未成熟和过分成熟的食品菜肴和水果,以及其它与个人体征不符的食物均应避免食用。

It was an unpleasant surprise when chemist William Brande succeeded in measuring the amount of alcohol in fermented drinks and not only proved that wine contained a higher percentage of alcohol than hard cider or beer, but also showed that the favorite American wine, Madeira, was more than 20 percent alcohol.

然而,化学家威廉姆·布兰德在发酵的饮品中也发现了一定含量的酒精,并证明葡萄酒所含的酒精比例要高于发酵汁和啤酒,而美国人喜爱的玛德拉酒含有20%多的酒精。这是个不愉快的消息,让人大吃一惊。

It was an unpleasant surprise when chemist William Brande succeeded in measuring the amount of alcohol in fermented drinks and not only proved that wine contained a higher percentage of alcohol than hard cider or beer, but also showed that the favorite American wine, Madeira, was more than 20 percent alcohol.

然而,化学家威廉姆·布兰德在发酵的饮品中也发现了一定含量的酒精,并不仅证明葡萄酒所含的酒精比例要高于发酵汁和啤酒,而且证明美国人喜爱的玛德拉葡萄酒酒含有20%多的酒精。

Whigs, eager to deliver what the public wanted, took advantage of this and declared that Harrison was "the log cabin and hard cider candidate," a man of the common people from the rough-and-tumble West. They depicted Harrison's opponent, President Martin Van Buren, as a wealthy snob who was out of touch with the people. In fact, it was Harrison who came from a wealthy, prominent family while Van Buren was from a poor, working family.

辉格党汲汲营营於给众人所需要的一切,利用这一点告诉人民哈里森就像是「住在木屋中、、喝含酒精苹果汁的候选人」(the log cabin and hard cider candidate)、和所有来自混乱西部地区的人们没有什麼不同,他们攻击哈里森的竞争对手、也就是当时的现任总统马丁范布伦,是个生来富裕的傲慢者、不了解一般人民的生活状况;事实上,哈里森自己才是出生於富有、显赫家庭的候选人,范布伦才是生於贫穷、劳工阶级的候选人。

Whigs, eager to deliver what the public wanted, took advantage of this and declared that Harrison was "the log cabin and hard cider candidate," a man of the common people from the rough-and-tumble West. They depicted Harrison's opponent, President Martin Van Buren, as a wealthy snob who was out of touch with the people. In fact, it was Harrison who came from a wealthy, prominent family while Van Buren was from a poor, working family.

辉格党汲汲营营于给众人所需要的一切,利用这一点告诉人民哈里森就像是「住在木屋中、、喝含酒精苹果汁的候选人」(the log cabin and hard cider candidate)、和所有来自混乱西部地区的人们没有什么不同,他们攻击哈里森的竞争对手、也就是当时的现任总统马丁范布伦,是个生来富裕的傲慢者、不瞭解一般人民的生活状况;事实上,哈里森自己才是出生于富有、显赫家庭的候选人,范布伦才是生于贫穷、劳工阶级的候选人。

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但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

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Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。