含血的

ALP would play a role in improving antioxidative ability of body,decreasing the damage of free radical to the body and maintaining the body weight of normal adult mice.The antioxidation of ALP maybe relate to Se,Vitamin E,β- carotin and flavonoids that ALP contained. 2.ALP has the different antioxidative mechanism from that Vitamin E has.

ALP 能提高正常小鼠血中 GSH-Px 活力及 SOD 活力，降低其 MDA含量(P.05)，有效防止受辐射成年小鼠血及肝脏中 GSH-Px 活力的降低和 MDA 含量的增加(P.05)，因此具有抗辐射、抗氧化的作用，ALP的抗氧化功能可能与其所含的硒、维生素 E、β-胡萝卜素以及异黄酮类物质有关。2。

Results:Normal rats as the donator of serum, using methanolic extract of CWC, 10 times equivalent dose for adults every day, intragastric administration twice a day of 8-hour interval, keeping on 3 days, hemospasia after 2 hours of the last administration was the better preparative method of the serum containing drug. Adding 10% of this serum to culture system could elevate hepatocyte increment and depress ALT in cell culture medium significantly.

结果：每天以成人等效剂量10倍的甲醇提取物间隔8h分两次给正常大鼠灌胃给药，连续3天，末次给药后2h采血制备含药血清比较合理，体系中加入10%该条件下制备的含药血清能明显提高损伤肝细胞的增殖能力、降低细胞ALT泄漏。

All rats were enforced intragastric administration of equivalent dose for 3 days. 1 hour and 2 hours later after the last intragastric administration, the serums of the G ranula of P enetrating Bone and Removing Pain were separated. Then we interfered in the obtained BMSCs for 72 hours with the serums from different density, and investigated the effect of the G ranula on proliferation of the BMSCs.（3） The second generation BMSCs were randomly divided into 3 groups, i.e.

给予相对应的给药量灌胃3d，末次给药分别在1小时﹑2小时后进行采血，制备透骨消痛颗粒含药血清，运用透骨消痛颗粒不同含药血清浓度对获得的BMSCs进行药物干预72h，通过MTT法研究透骨消痛颗粒对BMSCs增殖的影响。

To produce medicine serum of Liu-Shen-Wan with different dose, different time of pouring medicine, different time of blood sampling.2. To measure content of arsenic in serum with atomic absorption method, to qualitative analysis toadfish in medicine serum of Liu-Shen-Wan with thin layer chromatography.3. MTT and trypan blue assay detects the growth inhibition effect of Liu-Shen-Wan on HL-60 cells and to make certain the best scheme of making method of medicine serum of Liu-Shen-Wan.4. Gimsa dying assay observes morphology changes of apoptosis .In situ end-labeling method and flow cell machine quantificationally probes apoptosis phenomena.

1 分不同的给药剂量、给药天数和不同的采血时间制备含药血清。2 用原子吸收法测定血清砷含量；薄层色谱法定性检测六神丸含药血清中蟾酥成分。3 用MTT法、胎盘蓝染色法观察六神丸对HL-60细胞的增殖抑制作用，确定最佳血清制备方案。4 用吉姆萨染色法观察细胞凋亡的形态学变化，流式细胞仪、原位末端标记法定量检测细胞凋亡程度。

Aquatic crustaceans are typical ammonotelic animals. They excrete nitrogenous wastes in the form of ammonia. However, under high salinity, the hemolymph of Penaeus Monodon was found to contain a large quantity of urea. The aim of this thesis was to investigate the mechanism of how nitrogenous metabolites change under different salinities.

水生甲壳类是典型的排氨型动物，体内含氮废物以氮的型式代谢，但草虾於高盐环境下，其血淋巴中却产生大量的尿素，本论文研究的目的即在探讨含氮代谢物在环境盐度变化下的转换机制。

No difference were found for daily milking yield for among three groups also no difference for milk fat percentage between one fourth wild and domestic yak,but the milk fat percentage of semiwild is lower than that of o...

经q检验，3组的日均挤乳量和1／4野血牦牛与家牦牛的乳脂率均差异不显著（P＞0.05），而1／2野血牦牛因野性较大，难以挤净含脂较高的二奶，以致乳脂率低于其它两组。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。收获的NASC传代培养，用流式细胞仪检测细胞的免疫表型。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum. The acquired NASCs were subcultured for passage.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

METHODS: After isolated the PBMCs and blood-plasma from adult human peripheral blood by Ficoll-Hypaque centrifugation, the PBMC culture procedure with or without the self-blood -plasma was applied to polarize T cells in vitro , these cells were polarized by PHA(20 mg/L),non-PHA respectively.

用Ficoll-Hypaque梯度离心法分离出正常成人抗凝外周血单个核细胞和血浆，将PBMC置于含或不含有自身血浆环境中，并在植物血凝素(PHA ，2 0mg L)的刺激下进行培养，培养 2 4、48、72h后收获细胞，分别用双色免疫荧光标记，以流式细胞术对CD4+ T细胞表达表面分子CCR3、CCR5进行分析。结果：CD4+ T细胞受自身血浆和PHA刺激后向Th1 Th2两个方向极化，2 4、48、72h后极化率分别达到(13 2 8± 1 5 9)%(12 70± 1 6 5 )%、(17 19± 1 0 3)%(17 5 0± 1 30 )%、(19 49± 2 87)%(18 5 8± 1 49)%；而只有PHA刺激的极化率很低，两组有显著性差异(P 0 1)；Th1∶Th2之比约等于 1。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。