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Mature embryo sac can form from megaspore mother cell on the modified VW medium supplemented with 0.5mg/L BA and 0.1 mg/L NAA.

在培养基无外源激素或仅含生长素或细胞分裂素时,雌配子体的发生过程不能顺利完成;在改良VW培养基上添加0.5 mg/L BA和0.1 mg/LNAA时,形成成熟胚囊。

In this paper, we studied M. meretrix ferritin, cathepsin B and caspase genes, which are involved in clam larval shell formation, nutrition, metabolism and apoptosis, respectively. We have cloned the three genes, investigated the temporal and spatial expression profile both at gene and protein level in trochophore (L1), D-veliger (L2), pediveliger (L3) and postlarvae (L4). The potential roles of these proteins were analyzed with specific inhibitors during larval development. Firstly, embryos were found developed into trochophore-like larvae with no shell if cultured at gastrula stage in artificial seawater without iron. Shell-like structures were formed only in the presence of iron. The larvae which had been transferred at L1 stage into ASW developed normal shell. This indicated that iron and iron associated protein are important for larval shell formation. The EST sequence which is homologous with ferritin, which is a principal iron metabolic protein, was selected from the M. meretrix cDNA library. The full-length of ferritin subunit cDNA was cloned by RACE. The results of real-time PCR revealed that the MmeFer mRNA expression changed before and after the larval shell formation.

本论文以文蛤幼虫为研究对象,分别对文蛤幼虫发育过程中贝壳形成相关的铁蛋白、营养及变态相关的组织蛋白酶B及变态过程中细胞凋亡相关的caspase三个基因进行了克隆,分析了基因及编码蛋白在担轮幼虫期(L1)、D形幼虫期(L2)、壳顶幼虫期(L3)和稚贝期(L4)的时空表达特征,解析了其可能的功能,并研究了相应酶类的特异性抑制剂作用对幼虫发育过程的影响,进行了目标蛋白的功能验证,详述如下:研究结果显示,在文蛤胚胎发育到原肠胚时放入不含铁离子的人工海水中培养,发育成无壳的畸形,随着人工海水中铁离子添加浓度的升高,幼虫长出壳状组织接近正常状态;而发育到L1期幼虫放入不含铁离子的人工海水中培养却可以发育出正常的壳,推测铁和铁代谢相关蛋白在幼虫贝壳初始形成有重要的作用。

Sepal 5, fu covers with tiles shape is arranged, chang Sucun, corollaceous bell form, funnel form, tall foot butterfly form or earthen jar form, brim ministry is shallow 5 crack or deep 5 crack, rotate when bud or tweezers closes account; Staminal 5, on canister of move unripe Yu Guan, hide inside or appear, pollen bead does not have thorn or aculeate, on ovary, often be a cup of shape or surround for flower disc place of dissension, 1-4 room, every room contains 1-2 bead ovule, style 1-2, post is not cracked or 2 crack, rare 3 crack, or 2-4 is cracked.

萼片5片,覆瓦状排列,常宿存,花冠钟形。漏斗形。高脚蝶形或瓮形,檐部浅5裂或深5裂,芽时旋转或镊合状;雄蕊5枚,着生于冠筒上,内藏或外露,花粉粒无刺或有刺,子房上位,常为杯状或为分裂的花盘所包围,1-4室,每室含1-2粒胚珠,花柱1-2个,柱头不裂或2裂,稀3裂,或2-4裂。

Calluses were induced from hypocotyl segments of seedlings of Alhagi pseudalhagi on MS medium supplemented with 1. 5-2. 0mg/L 2, 4-D and 0.5-1. 0mg/L 6-BA.

骆驼刺种子无菌苗下胚轴切段在含1.5-2.0mg/L2,4-D和0.5-1.0mg/L6-BA的MS培养基中可100%诱导产生愈伤组织,并在相同培养基上进行继代培养。

In the study, embryo bud of Impatiens balsamina L was cultured on hormone-free MS medium for promoting, MS medium with 6-BA 3 mg/L+NAA 0.5 mg/L for shoot and root formation, and MS medium containing 6-BA 3 mg/L+NAA 0.5 mg/L for flowering respectively. During about 60 days, plant regeneration and flowering of Impatiens baisamina L was finished in vitro.

研究以凤仙花无菌苗胚芽段作为外植体,经过不含任何激素的MS(0)培养基预培养、MS+6-BA3mg/L+NAA0.5mg/L培养基的诱导芽和根分化、MS+6-BA1mg/L+NAA0.1mg/L培养基的诱导开花,在60d左右实现了凤仙花离体培养再生植株并在试管内开花。

The placenta element,Panax,glossy ganoderma,almond,angelica,dahurian angelica root,wheat germ oil and aminophenol,citrin,etc included in moistening and anti-wrinkle product can help to supply nutrient to the skin,improve flexibility and smooth the wrinkles,then make you looks to be younger.

保湿抗皱养颜产品中所含的胎盘素,人参,灵芝,杏仁,当归,白芷,小麦胚芽油和多种氨基酸,维生素等有效成份,能全面补充肌肤的养份,提高肤质的透明度和弹性,平得皱纹。

Objective To observe effects of Tuber Fleeceflower Root serum on activity of SOD, the expression of c-myc mRNA and the activity of telomerase of human fetal lung diploid fibroblasts (2BS).

目的 观察含何首乌血清对人胚肺二倍体成纤维细胞(2BS)超氧化物歧化酶活性、c-myc mRNA表达及端粒活性的影响。

Objective To observe effects of Tuber Fleeceflower Root serum on activity of SOD, the expression of c-myc mRNA and the activity of telomerase of human fetal lung diploid fibroblasts (2BS).

作者:李朝敢,韦星,农嵩,韦耀东,张树球目的观察含何首乌血清对人胚肺二倍体成纤维细胞(2BS)超氧化物歧化酶活性、c-myc mRNA表达及端粒活性的影响

Pure silicalite only exposed at surface is silica-bearing rocks of protore beds experiencing extreme oxidation at surface as additional production while generating oxidized manganese ores

纯硅质岩仅见于地表,是含硅质的矿胚层岩石,在地表经强烈氧化作用形成氧化锰矿石同时产生的&副产品&。

The transformed systems need not to have special forms and there are no restrictions on the types of nonlinearity contained in systems. The only requirement is that the two matrice defined in the paper should be a controllable pair at every point.

它不要求线性化微分同胚变换后系统具有特定形式,对系统所含非线性也不作限制,它只要求变换后系统的两个特定函数矩阵在点点均为能控对。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。