含胚的

The results indicated:0.1 mg/L 2,4-D or 0.1 mg/L 2,4-D+0.2 mg/L KT was optimal to induct the callus of carrot hypocotyls.The majority of the callus induced on the medium containing 0.1 mg/L 2,4-D regenerated embryoid,whereas,the callus induced on the medium containing 0.1 mg/L (2,4-D)+0.2 mg/L KT produced adventive buds.During the growth of regenerated plant,MS medium is available for rooting of seedling,and seedling regenerated on B5 medium must be placed on the B5 medium containing 0.1% IBA to develop root.

试验结果表明：0 1mg/L2，4 D或0 1mg/L2，4 D+0 2mg/LKT的激素组合有利于胡萝卜下胚轴的愈伤组织诱导；在0 1mg/L2，4 D的培养基上形成的愈伤组织主要以胚状体的形式再生，而在含0 1mg/L2，4 D+0 2mg/LKT激素组合的培养基上形成的愈伤组织主要以发生不定芽的方式再生；再生过程中，B5基本培养基上的苗子不易生根，需要附加0 1mg/L的IBA，而在MS培养基上苗子可直接形成根。

Receive what kind of colza, with respect to the straightforward production that send seed, both neither Qing Dynasty is miscellaneous, also not make friends embryo, make have some of oil plants contain miscellaneous quantity to be in 7 ％ above, exceed what the country sets to contain miscellaneous level.

收什么样的菜籽，就直接投籽生产，既不清杂，也不轧胚，使有些油料的含杂量在7％以上，超过国家规定的含杂标准。

Appling nonlinear feedback with estimated parameters and state diffeomorphism, Based on the Lyapunov approach we can develop a kind of novel robust controller designed for a class of nonlinear systems with mismatched uncertainty.

针对反馈可线性化系统，利用含估计参数的非线性反馈及状态微分同胚，基于李亚普诺夫方法给出了含非匹配不确定性系统的新型鲁棒控制器的设计方案。

Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法：成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒，手术后雄鼠与超排雌鼠合笼交配，用荧光原位杂变(Fluorescence in situ hybridization，FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制，用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction，RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

According to the year with the best large-seed production, the reasons for empty seeds could be estimated as follows: Seed potential per cone calculated as 2 times the number of fertile ovuliferous scales was about 206 seeds. Percentages of seed losses resulting from pre-and post-pollination ovule abortion, prezygotic factors, embryo degeneration, and efficient seed were 33%, 27%, 39%, and%, respectively.

依据大粒种子产率最高的1年来估测空粒种子产生的因素，可以推估如下：依据每个可孕性鳞片所含2粒种子的鳞片数，潜力种子数约有206粒，再依此值算出受粉前后胚珠败育率、受精前败育率、胚败育率、及有效种子率分别为33%、27%、39%及%。

The present study was conducted to investigate the effect of 6-DMAP on parthenogenetic activation rate, pronuclear formation and in vitro parthenote development of in vitro matured goat oocytes.

体外成熟的山羊卵泡卵母细胞用7%的乙醇和5μmol/L的离子霉素刺激处理后，分别置于含2 mmol/L 6-二甲基氨基嘌呤(6-DMAP)和不含6-DMAP的培养液中培养4 h，研究6-DMAP对体外成熟山羊卵泡卵母细胞孤雌激活率、原核形成以及孤雌胚体外发育潜能的影响。

Geochemical study indicates that constant elements, microelements and trace elements in manganese ores and protore wholly take on thermal sedimentary characteristics, the siliceous limestone, silica-bearing limestone and bioclastic limestone is predominant protore beds of manganese-capped ores.

通过对锰矿石和矿胚层岩石中常量元素、微量元素及稀土元素等地球化学的研究，均具有热水沉积的特征，硅质灰岩、含硅质灰岩和生物碎屑灰岩是锰帽型矿石最主要的矿胚层。

The genome of avian adeno-associated virus was cloned for construction of gene transfer vectors. AAAV was propagated in special pathogen-free chicken embryo using the chicken embryo lethal orphan virus as the helper virus. The double-stranded genomic DNA was extracted from precipitated AAAV viron and cloned into pCR 2.1 vector.

为了克隆禽腺联病毒(Avian adeno-associated virus， AAAV)全基因组用于构建基因转移载体研究，以鸡胚致死孤儿病毒作为辅助病毒与AAAV共接种SPF鸡胚进行AAAV的增殖，将AAAV约4.7 kb双链基因组DNA与pCR2.1载体连接，构建了含AAAV全基因组的重组质粒pAAAV并进行了测序。

It was found that the highest PAO activities in embryonic axes and radicles of the peanut seedlings occurred in day 4 and day 5 respectively after germination in dark. Additionally, the extraction medium of peanut PAO was 0.1 mol/L phosphate-buffered saline （pH 7.0）, containing 0.5 mol/L NaCl and 1 % polyvinylpyrrolidone.

结果表明：黑暗条件下萌发的花生幼苗，其胚根和胚轴的PAO活性分别在萌发后的第4 d和第5 d出现一个峰值；含1 % PVP和0.5 M NaCl的磷酸缓冲液（0.1 mol/L，pH 7.0）用作花生PAO分离提取液可得到较好的提取效果。

It was found that the highest PAO activities in embryonic axes and radicles of the peanut seedlings occurred in day 4 and day 5 respectively after germination in dark. Additionally, the extraction medium of peanut PAO was 0.1 molL phosphate-buffered saline （pH 7.0）, containing 0.5 molL NaCl and 1 % polyvinylpyrrolidone.

结果表明：黑暗条件下萌发的花生幼苗，其胚根和胚轴的PAO活性分别在萌发后的第4 d和第5 d出现一个峰值；含1 % PVP和0.5 M NaCl的磷酸缓冲液（0.1 molL，pH 7.0）用作花生PAO分离提取液可得到较好的提取效果。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失