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Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

The major bacteria of the activate sludge were identified to be Vorticalla convalleria and Aspidiacs spp., both were considered as responding to denitrification of high nitrate-N of lead azide wastewater.

中文摘要为了探讨含有高浓度硝基氮之叠氮化铅制程废水生物脱氮效能，本研究利用统一食品公司所提供的厌氧污泥进行脱氮菌的培养，经过了15天的培养其培养出大部份都是钟口菌属Vorticella convalleria ，和一小部份则为纤毛虫属aspidisca sp。，来进行含有高浓度硝基氮的氮化铅制程废水生物处理过程中的脱氮作用之研究。

In word processing, a program for producing form letters in which a name and address file is merged with the text file containing the letter.

在字处理技术中，用于产生形式化的信件的一种程序，这种信件是把含有姓名和地址的文件与含有信件正文的文件合并而成的。

If deg f = n, then Rfc is an empty set. For all the functions of degree 2, Rf have at least 2n-1 vectors, when Rf is a linear subspace, the relationships between Rf and Lf are discussed . Boolean functions have no nonezero linear structure if and only if there are n linear independence vectors in f, the correlation of the vectors in ?

证明了，若degf=n，则R_f~c为空集，对于所有的二次布尔函数而言，均有R_f~c中的元素个数大于等于2~(n-1)，给出了R_f构成线性子空间时，R_f和L_f之间的关系，还给出布尔函数不含有非零线性结构的充分必要条件是ζ_f中含有n个线性无关的元素。

For example red autumnal leaves is bright red broadleaf plant, red the flowers observing a part of a historical period such as back laurel, contain cyanine element inside their mesophyll cell, consequently the front of blade or the reverse side present red; The lamina that wait is in Gong Sang, nandina synthesis of the chlorophyll below weak light is much, kind of carotene falls in strong

例如红叶朱蕉。红背桂等观叶花卉，在它们的叶肉细胞内都含有花青素，因而叶片的正面或背面呈现红色；红桑。南天竹等的叶片在弱光下叶绿素合成多，在强光下类胡萝卜素合成多，故放在半阴处培养叶色发绿，放在强光下养护，则叶色发橙；金边吊兰。金边虎皮兰叶片的不同部位上细胞内分别含有叶绿素和叶黄素，所以叶片呈现出黄绿相间的两种颜色。

The result of the experiment showed that the qualitative medium - speed filter paper can be used as the material of the nitrite - detecting paper: color developing reagent A containing 4 - aminobenzene sulfonamide and N - 1 naphthyl ethylenediamine hydrochloride, and mixtures of 1 : 1 naphthylamine solution and sulfanilic acid solution as color developing reagent.

实验结果表明：定性中速滤纸可以作为亚硝酸根试纸的材料；含有4-氨基苯磺酰胺和N-1-萘基乙二胺盐酸盐的显色剂A及含有1：1的α-萘胺溶液和对氨基苯磺酸溶液的显色剂B，按1：1混合后可以作为显色剂；通过应用实验表明，亚硝酸根试纸具有体积小、便于携带、检测方便、成本低廉等特点。

It was indicated by UV-Vis spectra characterization that the precursor always contains the tetrahedral Ti species and the octahedral Ti species regardless of the Ti content. Calcination of the precursor resulted in a partial condensation of the octahedral Ti species to form the anatase phase.

UV-Vis 光谱表明，Ti-MWW 合成原粉中含有四面体的钛和八面体的钛，而八面体的钛属于一种骨架外钛，未经酸处理而煅烧会使部分八面体钛缩合形成锐钛矿，而经过酸处理和煅烧后的分子筛则几乎不含有锐钛矿。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Getting blood from vena cervicalis, making blood smear as routine, Giemsa staining were used to study through immersion objective observation on naturally case in clinic. The result indicated that many red blood cells infected T. Annulata in number from one to three, which infected one polypide take up the most and two the less, while that of three polypides take up the least.

对临床自然病例进行颈静脉采血，按常规制作血液涂片，Giemsa氏染色，油镜下观察和研究，结果表明：许多红细胞感染有1~3个数量不等的环形泰勒虫，以1个的为最多，含有2个虫体的红细胞较少，偶尔可见到含有3个虫体的红细胞。

It was found that the section was brittle. The scales of the pangolin were composed of the very thin prismy construction units and the folded units. The scales of the pangolin included such trace elements as S,Si,Fe,Al and Ca, besides the element of C,H,O and N. There were small amount of P element in the internal layer of the pangolin's scale.

结果表明冲击断口为脆性断口；穿山甲鳞片是由极细的棱柱结构单元和叠片结构单元混合形成的；穿山甲鳞片除含有大量的碳、氢、氧及氮元素外，硫、硅、铁、铝、钙5种元素的含量也相对较高，鳞片内层中含有少量的磷元素。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。