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The invention discloses an application of polyhalogenated acridone compound in a buffer solution as a pH fluorescent probe for high selective identification of hydrogen ions.

本发明公开了一种多卤代吖啶酮类化合物作为pH荧光探针在缓冲溶液中对氢离子高选择性识别的应用。

In this thesis, investigations focused on heteropoly acids-catalyzed C-C and C-N bond formation reactions and the synthesis of xanthenediones and acridinediones under thermal conditions are described.1.12-Phosphotungstic acid has been used as an efficient,eco-friendly,and air- and moisture-stable catalyst to promote the direct dehydrative substitution of the hydroxy group of benzylic and allylic alcohols with variousβ-dicarbonyl compounds.

本篇论文主要描述了杂多酸催化下的C-C和C-N键的生成,以及无溶剂直接加热下合成十氢氧杂蒽二酮和9-芳基十氢吖啶二酮。主要内容如下:1。在磷钨酸催化下,1,3-二羰基化合物与醇能够顺利偶合,脱除一分子的水,生成烷基化的1,3-二羰基化合物。反应在甲苯中进行时,二芳基甲醇能与各类1,3-二羰基化合物反应,成率很高;但对于1-苯乙醇,只能得到很低的产率。

Escherichia coli O157:H7; viable but non culturable state; acridine orange direct count; direct viable count

H7;活的非可培养状态;吖啶橙荧光显微镜直接计数法;活菌直接镜检计数法

Methods: Thirty patients with malignant hemopathy were detected using modified technique of R-banding, and the caryotype of the cases was studied. Acridine orange solution was substituted with 10% Giemsa, the composition of the reagents was readjusted and the procedures of the experiment were simplified.

用改良的染色体R显带方法对30例各种类型的染色体进行显带,并对显带的染色体核型进行研究;将传统R显带技术中用10%吉姆萨染液取代吖啶橙工作液,调整欧氏液各种试剂成分和简化试验步骤。

It is found that the sensitivity of the photosensitive system with N-alkylthioacridone is improved strongly by further addition of diphenyl iodonium salt and actibator.

由硫代吖啶酮、二苯基碘鎓盐和活化剂组成的共引发体系使光聚合涂层的感度大大提高

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞;以不同浓度的LPS(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml)和不同作用时间(0min,5min,15min,30min,60min,120min)分别刺激小室培养的细胞单层,观察NF-κB的核内浓度及NO合成量的动态变化,选择LPS的最佳用量和作用时间;然后分成四组实验,设正常对照组,LPS处理组,特异性PKC抑制剂阻断组,NF-κB抑制剂阻断组;收集培养的单层细胞及培养液;采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平;免疫组化法检测NF-κB的核内移位变化;硝酸还原酶法测定细胞培养液中NO含量;吖啶橙染色观察凋亡细胞的形态学变化,凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

Organocatalyst ; N-methylimidazole ; aromatic aldehyde ; multi-component reaction ; Knoevenagel condensation reaction ; ionic liquid rhodanine ; decahydroacridine

广东省自然科学基金;国家自然科学基金有机小分子催化剂; 1-甲基咪唑;芳香醛;多组分反应; Knoevenagel缩合反应;离子液体;罗丹宁;多氢吖啶

A series of 3,3,6,6 tetramethyl 1,8 dioxo 9 aryl 1,2,3,4,5,6,7,8,9,10 decahydro acridines have been synthesized by the condensation of aromatic aldehydes, 5,5 dimethyl 1,3 cyclohexanedione and ammonium acetate in the presence of triethylbenzylammonium chloride as catalyst in water.

芳醛、5 ,5 二甲基 1,3 环己二酮与醋酸铵在三乙基苄基氯化铵催化下以水作为溶剂反应,生成一系列 3 ,3 ,6,6 四甲基 1,8 二氧代 9 芳基 1,2 ,3 ,4,5 ,6,7,8,9,10 十氢吖啶,与其它溶剂相比,水作为该反应的溶剂具有价格便宜,不易燃和无毒等优点,而且产率较高,产物的结构通过单晶X射线确

At the same time, we studied how the interaction of Sm3 with DNA was effected by the changes of pH and salt number. Scatchard plots show uncompectitive inhibition of Sm3 hinging to DNA.

同时研究了酸度、盐效应等对Sm3与DNA相互作用的影响,Scatchard图表明该配合物对吖啶橙与DNA的结合为非竞争性抑制。

The products were identified by IR,NMR,MS and elemental analysis. In our method,K.OH was used in place of NaOH to synthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate was purified on a silica gel column with chloroform/ethylacetate(4:l,v/v) as eluent and further purified by triturating with hexane/acetone(2:l,v/v).The luminescence produced by DMAE-NHS is a flash light with maximumemission at 0.4s and decay half-time of 0.9s. The luminescence intensity is 6.11x10 cps/mol,which is affected by the composition of trigger and surfactant.

DMAE·NHS的合成是本论文的关键和难点,我们对文献方法进行改进:文献方法用氢氧化钠与3,5-二甲基-4-羟基苯甲酸反应制得3,5-甲基-4-羟基苯甲酸钠,再用3,5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3,5-二甲基-4-羟基苯甲酸苄酯,我们用氢氧化钾代替氢氧化钠,使合成取得成功;在合成2',6'-二甲基-4'-苯基-吖啶-9-甲酸酯时,文献方法对粗产品进行两次硅胶柱层析纯化,第一次柱层析用氯仿/乙酸乙酯(4:1,Ⅴ:Ⅴ)作溶剂和淋洗剂,第二次柱层析用己烷/丙酮(2:1,Ⅴ:Ⅴ)作溶剂和淋洗剂,按照文献方法得到的不是所需要的化合物,我们只进行第一次柱层析纯化,然后用己烷/丙酮(2:1,Ⅴ:Ⅴ)进行研磨,过滤,洗涤,除去溶于己烷/丙酮(2:1,Ⅴ:Ⅴ)的部分,得到所需要的产品。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。