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The reaction between acridine yellow and nitrite nitrogen in acid medium was studied.

利用在酸性介质中,吖啶黄与亚硝酸根反应并在波长436 nm处的吸光度与亚硝酸根浓度呈线性关系,提出并建立了测定亚硝酸盐氮的新方法。

The Cardo structure could increase the solubility and thermal stability of the resultant polyimide.

通过在聚酰亚胺的主链中引入庞大侧基(9,9-二苯基芴)形成轴状聚酰亚胺,同时提高聚酰亚胺的溶解性和热稳定性;庞大的侧基又能阻止链的堆砌,避免生色团荧光链间淬灭;通过加入第二种二酐单体合成三元缩聚型聚酰亚胺,调控荧光性二酐在聚酰亚胺中的含量,降低生色团在聚酰亚胺中的浓度,来避免生色团荧光浓度淬灭,增加聚酰亚胺的固态荧光量子效率;通过使用热稳定性好的稠环生色团苝和吖啶来避免共轭类生色团(如含双键或三键的生色团)在高温下热交联及氧和水的破坏。

The existence of hydrogen bond within or between the molecules of pigment can enhance the perdurability, light fastness and weather resistance.

有机颜料发色母体最主要的是偶氮和酞菁两大类(分别为50%和25%),其余25%的颜料属于杂环的发色体:喹吖啶酮、异吲哚啉与异吲哚啉酮、苝、喹酞酮、二噁嗪、吡咯并吡咯二酮、金属络合颜料、靛族和稠环酮颜料(如:蒽醌、黄蒽酮、皮蒽酮等)。

ABSTRACT : Objective To establish a determination method for octahydroaminoacridine succinate.

目的 建立琥珀八氢氨吖啶原料药的含量测定方法。

The above two methods are simple, accurate and precise for the determination of octahydroaminoacridine succinate.

结论这2种方法简便、准确、重现性好,为琥珀八氢氨吖啶原料药的含量测定提供了方法。

To explore the clinical reliability and usability of the acridinium ester-labeled CLIP and ELISA in detection of AFP to establish a specific, effective, simple and reliable detecting method in our lab.

[目的]探讨吖啶酯标记的化学发光免疫测定(chemiluminescence immunoassay, CLIA)分析技术与酶联免疫吸附试验一步法两种方法检测甲胎蛋白(Alpha-fetoprotein, AFP)的临床可靠性、适用性。建立本室特异、有效、方便、可靠的试验方法。

CLIA using acridinium ester derivatives as chemiluminescent label has advantages of low background,high signal-to-noise ratio,no need of catalyst and simple luminescence system.

吖啶酯衍生物为发光标记物的CLIA的特点是:标记简单、标记物稳定、不需要催化剂、自然本底低、受外界干扰因素少、信噪比高、发光体系简单等。

Different aspects associating the DMAE-NHS-based CLIA were studied in this thesis,including synthesis of acridinium ester,chemiluminescent characteristics of DMAE-NHS,labelling antibody or streptavidin with DMAE-NHS,two-site sandwich chemiluminescent immunoassay for TSH,two-site sandwich chemiluminescent immunoassay involved biotin-streptavidin system for TSH,and competitive chemiluminescent immunoassay using biotin-streptavidin system for TLVThe desired acridinium ester,DMAE-NHS,was synthesized according to the reference method with some modifications.

目前国内尚未见有关DMAE·NHS的合成及其应用于CLIA的研究报导,本论文研究了吖啶酯DMAE·NHS的有机合成方法,DMAE·NHS的发光性能,DMAE·NHS及其与抗体偶联物的热稳定性,DMAE·NHS标记TSH单克隆抗体和链亲和素,TSH CLIA,TSH BAS-CLIA和TT_4 BAS-CLIA。

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点,我们对文献方法进行改进:文献方法用氢氧化钠与3,5-二甲基-4-羟基苯甲酸反应制得3,5-二甲基-4-羟基苯甲酸钠,再用3,5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3,5-二甲基-4-羟基苯甲酸苄酯,我们用氢氧化钾代替氢氧化钠,使合成取得成功;在合成2',6'-二甲基-4'-苯基-吖啶-9-甲酸酯时,文献方法对粗产品进行两次硅胶柱层析纯化,第一次柱层析用氯仿/乙酸乙酯(4∶1,V∶V)作溶剂和淋洗剂,第二次柱层析用己烷/丙酮(2∶1,V∶V)作溶剂和淋洗剂,按照文献方法得到的不是所需要的化合物,我们只进行第一次柱层析纯化,然后用己烷/丙酮(2∶1,V∶V)进行研磨,过滤,洗涤,除去溶于己烷/丙酮(2∶1,V∶V)的部分,得到所需要的产品。

The acridone could be quenched by the diphenyl iodium salt and the electron transfer mechanism between them was verified via fluorescence quenched.

实验又证明了由硫代吖啶酮、二苯基碘盐和硫代水杨酸三者组成的光聚合引发体系的引发效果最好。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。