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In the present study, we demonstrated that the mouse embryonic stem cells were differentiated into ciliated epithelial cells, with characteristics of normal ciliated cells.

通过我们的研究,实现了由小鼠胚胎干细胞向纤毛上皮细胞的分化,并且分化细胞具有正常纤毛细胞的特性。

Meanwhile, utilizing cells derived from same germ layer to functional replace the original cell types in the defect area has provided a great hint for seeking new cell sources.

而利用发育同源细胞在特定组织为环境下向特定细胞的转变以实现功能上的替代,为寻找种子细胞来源开辟了新的思路。

This is a simple and easy method for induction, cultivation and identification of human monocyte-derived macrophage.

人外周血单核细胞在rhGM-CSF诱导下向巨噬细胞分化,具有生物学活性,纯度较高,是一种简单易行的体外诱导培养巨噬细胞的方法。

By Prussian blue staining, differentiation into vascular endothelial cell and cardiocyte-like cells could be found under light microscope and Feridex could be englobed by macrophages.

普鲁士蓝染色镜下可观察到Feridex标记的BM-MNCs向心肌样细胞和血管内皮细胞的分化,Feridex可被巨噬细胞吞噬。

The growth speed of the cells with transfected Tob gene got slowly, even to a growth arrest.

结论Tob基因转染后的胶质瘤细胞生长受到明显抑制,出现向正常胶质细胞的分化和凋亡现象,提示Tob基因确实是一胶质瘤细胞增殖抑制基因,值得进一步做功能研究和实验性基因治疗研究。

BACKGROUND: The study on regulation adipogenic differentiation of adipose tissue-derived stromal cells is benefit for artificial control differentiation direction of adipose stromal cells, and promote further understanding of cell differentiation.

背景:触发和控制脂肪基质细胞脂向分化的调控研究将有助于人为控制脂肪基质细胞的分化方向,促进对细胞分化的深入了解。

Results: The study showed that the earliest stromal nodules were fibrous, and with the dual differentiation of myofibroblasts toward both smooth muscle cells and fibrous cells, they developed into fibromyomatous nodules and muscular nodules. The results of reclassifying the 55 nodules immunohistochemically were that 23(42%) was fibrous, 28(51%) fibromuscular nodules and 4(7%) muscular.

结果:结合HE切片和免疫组化结果发现前列腺间质结节的早期为幼稚的纤维性结节,随着结节内肌纤维母细胞向平滑肌细胞和纤维细胞的双向分化,间质结节可继续演化为纤维肌性结节和肌性结节。55个结节按免疫组化分类,纤维性结节占42%,纤维肌性结节占51%,肌性结节占7%。

And the long axis of simple pit Varied along with CMfs orientation of each secondary wall.This paper proposed a structure model of Phyllostachys pubescens cell wall,based on the results of CMfs deposition and thickness of each secondary wall.Secondly, it was a multilamellate structure of parenchyma cell wall,but its thin and thick lamellate was not evident.CMfs orientation on primary wall of parenchyma cell was perpendicular to cell axis.

纤维的细胞壁为典型的薄厚层相间的同心圆多层结构,初生壁CMfs排列方向与细胞轴向垂直90°次生壁中厚层的CMfs均以接近平行的小角度(0~5°沉积;薄层一般以大角度沉积,且薄层的CMfs角度由壁外侧向细胞腔里逐渐增加,直到完全垂直。

When pea ( Pisum sativum L.,Alaska strain) was grown in short\|day conditions,the apical bud senescence started with the transformation of the bud from vegetative to reproductive growth.DNA fragmentation was detected during the senescence using the terminal deoxynucleotidyl transferase\|mediated dUTP nick end in situ labeling method.The cell ultrastructural changes were similar to apoptosis in animal cells:the apical meristemetic cells underwent the programmed cell death.This wa...

显微、超微结构研究表明,短日照条件下豌豆顶芽的衰老过程是从营养生长锥向花芽的转化,而用DNA原位末端标记、Caspase 8WesternBlot和 14 0bpDNA片断积累的试验结果证明,转化为花芽的整个生长锥细胞发生了编程性死亡,而且其最顶端部分细胞首先发生PCD ,而顶端周围的分生组织细胞逐渐分化出花芽的各部分,但顶芽最后并没有发育成为完整的花,所有细胞就都发生PCD ,从而顶芽衰老

The animal model of this method system have, the characteristics of the high fat, high leptin, high Ins, and match with the characteristic of clinical and the pathologic of simple obesity. This studies showed: The acupuncture can obviously reduce the vaule of intaking food and water of the fat rats, and lower the body weight and fat; The acupuncture can urge the diameter, area, physical volume of white fat cell contract, Brown fat tissue of fat rat group surroundings vascular containing large quantity of white fat cell, the acupuncture group was less. this showed: The acupuncture enhanced the white fat cell metabolism, and promotesed the white fat cell to convert to brown fat cell; The frequency of spontaneous electric of nerve cell of pvn of the fat rat is high, the acupuncture counld depress the spontaneous electric of nerve cell of pvn, This showed: the acupuncture can depress the appetite passing to adjust function of pvn nerve; The monoaminergic neurotransmitter of inside of the fat rat brain is disfunction, the acupuncture can rectify this disfunction, and adjust level of 5-HT, and affect appetite, and improve metabolism; The level of leptin and Ins of fat rat increased high, The level of it of acupuncture group decreased, compared with the fat group p.001, This showed: the fat rats have the defeat of leptin and Ins in the body, The acupuncture can lowering this defeat, The level of leptin and Ins of the fat rat in the brain is less than the normal group, The level of leptin of the acupuncture group is high than the fat group, This showed: the acupuncture can increase the transportation of leptin from blood to brain.

本方法所制肥胖大鼠模型具有高体重、高脂、高leptin和高Ins血症的特点,符合人类单纯性肥胖病的临床和病理特征;实验研究显示:针灸能明显减少肥胖大鼠的摄水、摄食量及降低其体重、体脂;针灸能促使白色脂肪细胞直径、面积、体积缩小,减少肥胖大鼠棕色脂肪组织血管周围白色脂肪细胞数量,显示:针灸增强了白色脂肪细胞的代谢,促进了白色脂肪细胞向棕色脂肪细胞的转化:肥胖大鼠PVN神经细胞自发放电频率增加,脑内单胺递质紊乱,针灸通过抑制PVN亢奋的自发放电,调整5-HT水平,达到影响食欲,改善代谢的作用;肥胖大鼠血中leptin及Ins水平增高,与正常组大鼠比有明显差异,提示大鼠体内存在leptin及Ins抵抗,针灸后肥胖大鼠随着体重体脂的下降,血中leptin、Ins水平降低,与肥胖组比均有显著性差异,而针灸后大鼠脑内leptin增加,与肥胖组比P.05,提示:针灸具有改善leptin、Ins抵抗及增加leptin脑内转运的作用。

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