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Intracellular recordings were made from neurons in the superfused isolated carpretina and the effects of calcium on synaptic transmission from cones and rods to horizontal cells were studied by changing the ionic composition of the external medium.

工作应用细胞内记录技术,在灌流的卿鱼离体视网膜的标本上,研究了低钙对外网状层中视锥、视杆信号向水平细胞传递的不同影响。

About 4 days post amputation, cells in some areas in the out epidermis and inner parietal peritoneum near the end of the stump are induced by injury to dedifferentiate into stem cells and then proliferate forming stem-cell masses, which then invaginate into the interior dermis. The dermal cells are then induced by those invaginating stem-cell masses to dedifferentiate.

从第4 d起,近创伤处的表皮层和体腔上皮中部分细胞发生了脱分化形成了干细胞,并增殖形成了干细胞团,然后向真皮层内陷,并诱导真皮层组织发生脱分化,最终在残腕体壁的不同部位形成了众多干细胞原基,是进一步再生所需的细胞库。

RESULTS: There were many vacuoles and cracks in the cells, with heterochromatin margination, nuclear pycnosis and nuclear fragmentation. The high intensity pulsed electric fields caused damage in K562 cells, and the apoptotic percentage went up with the increase in frequency and intensity of PEF.

结果:经过高电压脉冲电场处理后的K562细胞,胞内出现大量空泡及裂隙,核内染色质向核周边聚集形成凋亡小体,呈典型细胞凋亡形态学改变;K562癌细胞的凋亡比例随着脉冲个数和电场强度的增加而增加。

In patch clamp experiments, for measuring the activities of live cells in various physiological environments, it is indispensable to perfuse a wide range of drugs to live cells.

膜片钳实验中,为了检测细胞在不同的生理环境下的生物活性,需要向活体细胞施加各种不同的药品。

The G ranula of P enetrating Bone and Removing Pain can significantly promote the chondrogenic phenotype differentiation and optimize the seeding cells in cartilage tissue engineering system and improve the quality of cartilaginous tissue and postpone the changes of retrogression in cartilage.

3透骨消痛颗粒可以促进BMSCs向软骨细胞转化,优化骨组织工程的种子细胞体系,改善软骨组织的质量,延缓软骨的退变过程。

These results suggested that theoverexpression ofA VPI increased sequestration of Na~(-1) into vacuole and uptake capacity of other cations in the transgenic alfalfa.

这些结果说明AVP1的超表达促进了转基因紫花苜蓿细胞中Na~+向液泡中的区域化,并增强了细胞对其它阳离子的吸收能力。

In long-term primary culture, epithelial cells from lenses with more advanced cataractous degree showed a relatively slower cellular extension from the explant and presented signs of cell aging more rapidly with time.

在长期初培养时,从较重程度白内障水晶体取得的上皮细胞自前囊向外生长的速度较缓慢,而细胞老化现象的出现较快速。

By Percoll fluid and density gradient centrifugation,the MSC was obtained;after the cells filled the bottom of vessel,subcultured them,when they subculture in third generation ,redigested them,500 R/min centrifugate,alter the

经percoll液密度梯度离心法能获得纯度较高的人骨髓间充质干细胞,这种细胞在体外培养体系中保持旺盛的增殖能力;经轻度离心和化学限定培养基法可诱导骨髓间充质干细胞向成软骨方向分化,增殖旺盛,可传一代培养,是软骨组织工程理想的种子细胞。

We have found that microfilament of cytoplasm was decreased or disappeared and rate of lateral diffusion of ConA r ceptor was increased (D = 1.05 ×10-10cm2/ sec)after the cells were treated with CB. The microfilaments and the rate of lateral diffusion of ConA receptor on cell membrane were recovered D = 0.81 ?

经CB处理细胞后,胞质微丝减少或消失,但是膜ConA受体复合物的侧向扩散运动增加〔D〕=1.05×10~(-1)cm~2/sec,当除去CB后,微丝重新出现,膜ConA受体复合物的侧向扩散运动又减慢〔D〕=0.81×10~(-1)cm~2/sec,与对照组相似。

MSC can be obtained by percoll fluid and density gradient centrifugation, this cells kept proliferation and differentiation ability in vitro; MSC can differentiate into chondroblast by lightly centrifugation and chemical definition medium, after one generation, the differential cells kept the phentype of chondroblast, they are a kind of ideal seed-cells for cartilage tissue engineering.

经percoll液密度梯度离心法能获得纯度较高的人骨髓间充质干细胞,这种细胞在体外培养体系中保持旺盛的增殖能力;经轻度离心和化学限定培养基法可诱导骨髓间充质干细胞向成软骨方向分化,增殖旺盛,可传一代培养,是软骨组织工程理想的种子细胞。

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