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The cell to cell contact could facilitate the transformation. It was found that the B. subtilis strain, cultured not only in MM medium, but also in LB medium, could be transformed by cell released or chemically extracted DNA by the method of agar plate transformation, although the LB medium was previously believed not to support this process in traditional transformation protocol.

结果表明,该菌在这两种培养基中培养时均可在对数生长期向培养液中主动分泌有生物活性的DNA,且细胞间接触有利於转化过程的进行,暗示在本实验条件下DNA供体细胞可能具有某种主动功能。

The cytoskeleton was observed with Rhodamine-Phalloidin staining and lateral mobility of ConA receptor complex was determined by the method of FRAP. The experiment was divide into 5 groups.

本实验采用体外培养的人胃癌细胞,利用免疫荧光技术和FRAP技术研究细胞骨架和膜表面ConA受体复合物的侧向扩散运动。

Methods The model of intravasation migration of cancer cell in vitro was used. The fibrosarcoma cell HT1080 was labelled with fluorescence, and the fluorescence guantities in 24-pore plate through collagen membrane and HUVEC single cells layer were detected in different time to eveluate the intravasation migration of cancer cell.

利用体外癌细胞向血管内游走模型,将纤维肉瘤细胞HT1080经过荧光标记,通过测定不同时间穿过胶原层及HUVEC单细胞层落到24孔板内的荧光量来定量评价瘤细胞血管内的游走情况。

Objective: The objective is to observe the expression of HOXC4 gene、HOXC6 gene on the differentiation and proliferation of hematopoietic stem cell to colony Colony Forming Unit-T Lymphocyte in vitro. And the differentiation progress affected by HCMV and/or ATRA, and to explore the possible mechanism of HCMV-induced maldevelopment to human cord blood T-Lymphocyte progenitor in genic level.

目的探讨人类脐血造血干细胞向淋巴系祖细胞增殖过程中HOXC4、HOXC6基因表达的情况,并且用人类巨细胞病毒和/或全反式维甲酸进行干扰,在基因水平探讨HCMV感染导致脐血淋巴系祖细胞损伤的机制。

Horny pearls and parakeratosis cells in cancerous nests centres were stained negative. The positive cells were distribution of mass, even diffusion, in moderate and poor differentiation.

在恶性度较高的肿瘤中阳性细胞呈团块状或片状灶性分布,甚至于呈弥散性分布,在肿瘤向周围组织浸润的边缘区阳性细胞最为密集。

Stone cells were developed from parenchyma cells of fruit flesh from interior to exterior.

石细胞是由薄壁细胞转化而来的,转化的顺序是从果肉内部逐渐向外。

By disrupting the impermeability of the sarcolemma, extracellular cations and fluid flow down the electrochemical gradient into sarcoplasm, overwhelming the capacity of the cationic extrusion pumps14–18 and leading to swelling of the myocytes.

肌纤维膜的不渗透性被破坏,细胞外阳离子与液体向肌浆流入,超过阳离子的泵出能力,导致肌细胞水肿。

Objective To explore an experimental method of transfecting the marrow stromal stem cells with the reconstructed PGL3-transforming growth factor-β1 (TGF-β1) gene and to evaluate the feasibility of self-induction of MSCs to the chondrocytes in vitro so as to provide a scientific and experimental basis for a further "gene enhanced tissue engineering" research.

目的 探讨将编码细胞转化生长因子β1(transforming growth factorβ1,TGF—β1)的重组PGL3-TGF-β1质粒转染兔骨髓基质干细胞(marrow stromal stemceils,MSCs),体外通过自分泌、诱导作用向软骨细胞方向分化的可行性,为基因加强组织工程学修复软骨损伤提供体外实验依据。

Fig.5 Late stage in differentiation of spermatid,the nucleus migrate towards the zone of differentiation.

图5。精细胞疼痛分化的晚期长形的细胞疼痛核移向分化区,领状结构。13000

In fish and amphibia, the transition from maternal gene transcription to zygotic transcription happens during the time of midblastula formation, this stage is named by midblastula transition, at this period, embryo cell cycle composition changes, it is the first time to G1 time appearance, the cell cycle timelimit lengthens and the cell division synchronism loses, the large quantities of zygotic gene transcription starts.

鱼类和两栖类由母型调控向合子型调控过渡发生在中囊胚形成阶段,称为中囊胚过渡(midblastula transition,MBT),胚胎细胞周期时相的组成在此期间发生变化,首次出现G1期,周期时限延长,细胞分裂的同步性丧失,大批合子型基因活化转录也在此时开始。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。