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The density of AR of HFSC is consistent with the capability of differentiation of those cells. 3. Cells induced in vitro expressed EMA and Lipid droplets were observed by rathonum red staining, while those as control didn't, which confirmed that androgen can facilitate the differentiation of vibrissa follicle stem cells into sebocytes.

用免疫细胞化学检测到EMA在诱导后的细胞中的表达呈阳性,而在未经诱导处理的细胞中的表达呈阴性;用油红O染色检测到诱导后的细胞中有脂滴的形成,而在未经诱导的细胞中没有脂滴的形成;表明雄激素对毛囊干细胞向皮脂腺细胞定向分化具有促进作用。

Stratum granulosum: outside the Spinous layer, made up of cells further differentiated to cuticle cells, so it would lose the nucleolus and turn to the enucleated startum lucidum and horny layer after completely cornified to horny layer.

颗粒层:位于棘层之外,是进一步向角质层细胞分化的细胞,因此它起着向角质层完全角化后,便失去细胞核,而转化成无核的透明层和角质层。

The location and structures of sex-pheromone-producing gland in female H.insularis were studied by EAG,GC,SEM,and TEM.These studies showed that thegland situate in the intersegmental membrane between the eighth and ninthabdominal segments,and is an eversible abdominal fold;Many plump cones disturbon the surface of the gland.The glandular cells of 2-day old virgin female H.insularis are arranged in one layer,among which the central cells are columnarepithelial cells and flat on two sides.The nucleus is irregular elliptical.There isevident conjugation between cells and the involution is more in the basal membraneof cell.Microvilli are distributed on the cytoplasmic membrane and linked withendocuticle on which there are many layers of chitin,and the outer cuticule is staineddeeper.The cell contains bubbles,mitochondria,glycogen deposits,roughendoplasmic reticulum and smooth endoplasmic reticulum.

结合触角电位、毛细管气相色谱、扫描电镜、透视电镜等技术对小线角木蠹蛾雌蛾腹尖末端不同组织部位提取物的测定分析以及腺体位置和形态结构的观察发现:小线角木蠹蛾性信息素分泌腺位于腹部末端8~9节之间,是一个由节间膜特化而成的上皮结构,为一可外翻的腹褶,腺体表面分布着饱满的锥形体,羽化后2天未交尾的雌蛾腺体细胞呈单层排列,腹面中央由密集的柱形上皮细胞组成,细胞排列向两侧延伸至背部,其形状由柱形逐渐变为扁平形,细胞核为椭圆形,细胞与细胞间有明显的胞连接,细胞基底膜基褶较多,质膜上分布着微绒毛,并与内表皮连接,内表皮上有多层几丁质,外角质层染色较深,细胞质中含有空泡、线粒体、脂质粒、粗面内质网和光面内质网。

MSC has multipotential that it can differentiate into fibroblastic, osteogenic, adipocytic, and other cell lines.

MSC具有向多种组织细胞方向分化的潜能,如何诱导其向成骨细胞转化是本研究的关键。

Some recent researches of abroad have found bone marrow cells can also differentiated into glomeruli cells in some conditions, differentiated into intercapillary cells, endothelium or podocyte in different animal models, but these reports are rare in the domestic.

国外的一些研究表明,骨髓细胞在一定条件下还可以向肾小球细胞分化,在不同的动物模型中分化成为肾小球的系膜细胞、内皮细胞和足细胞,但国内却鲜有报道。

Generally we think muscular histogenesis has many steps of biology, including foetal mesoderm merotomy; myoblast shapes in arthromera; myoblast can form muscular tissue to move to limbs; the proliferation of sarcoblast can increase cells\'quatity.

一般认为,肌组织发生是一多步骤的生物学过程,包括胚胎中胚层的分节;成肌细胞在体节形成;成肌细胞向四肢迁移形成肌组织;成肌细胞增殖以增加细胞数量。

C2C12 cells were induced to differentiation into muscle cells in vitro and the myogenesis model was detected by inverted microscope, RT-PCR, western-blot, cell immunofluorescence histochemistry, flow cytometry and confirmed to be successful. On the foundation of differentiation model, miR-206 was detected by northern hybridization and the result showed that the expression level increasing gradually during muscle differentiation with a character of phase specificity.

利用C2C12细胞在体外成功诱导其向成肌细胞分化,通过形态学观察,RT-PCR、western-blot、细胞免疫荧光组织化学及流式细胞仪等方法进行检测,证实我们所建立的细胞分化模型是成功的,并观察到随着诱导时间的延长,miR-206在成肌分化过程中表达量逐渐增高的,表达改变具有阶段特异性。

The biological features of hBMSCs and cryogentic storaged cells were analyzed.hBMSCs memberane antigens were identified by immunofluorescence staining.The potential osteogenous differentiation of hBMSCs was evaluated by the osteogeneic inducer kit.

倒置光学显微镜下观察细胞形态及生长情况,细胞计数法绘制细胞生长曲线,并分析冻存细胞复苏后的生长特性,免疫荧光染色测定hBMSCs表面标志,成骨诱导试剂盒检测hBMSCs向成骨分化的潜能。

Results The results showed that fibroblasts were the main source of extracellular matrix production in bile duct wall. The phenotype of fibroblasts in inflammatory strictured bile duct wall changed obviously, quiescent fibroblasts were activated and transformed to myofibroblasts, with massive proliferation.

结果 成纤维细胞是胆管壁中合成胶原的主要细胞;炎性狭窄胆管壁的纤维化增厚明显,其成纤维细胞表型改变,由相对静止转化为功能活跃状态,并进一步向肌成纤维细胞转化,且大量增殖。

Results Compared with normal group and sham operated control groups, BrdU positive and Nestin positive cells were increased in the hippocampus, dentate subgranular zone and subependymal zone in operated groups after 24 hours of reperfusion of omni-cerebral ischemia.

结果 脑缺血再灌流24 h后,海马、齿状回和室管膜下区的BrdU阳性细胞和Nestin阳性细胞增多,7~10 d达到高峰,术后20 d仍有表达;在室管膜下区,BrdU阳性细胞和Nestin 阳性细胞有向皮质、海马迁移的现象。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

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