向细胞的

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

BMSCs may reverse the disbalance of Th1/Th2 cells by regulating the cytokines production to take an important part in treatment of EAE.

在体外BMSCs通过化学诱导可以向少突胶质细胞分化；在体内BMSCs能向病变部位迁移，并部分分化成少突胶质细胞，同时BMSCs通过调节免疫系统的炎症细胞因子表达、CD4+和CD8+T细胞数和Th1和Th2细胞平衡，对EAE起治疗作用。

The wood structures of Lauraceae were characterized by most diffuse porous; most pores solitary, less multiple and cluster pores; simple and scalariform perforation with few bars; most solitary pore; alternate intervessel pitting; most of rays multiseriate, less uniseriate; all of rays heterogeneous, mostly heterogeneous Ⅲ and Ⅱ type rays; most of axial parenchyma paratracheal type, less banded or marginal type; oil cell and mucilage cell in axial parenchyma cell or ray cell; mostly fiber tracheid and libriform fiber, less septate fiber.

结果表明：除檫木外，其它木材的管孔分布均为散孔材，具较高的单孔率；导管分子穿孔板兼有单穿孔和梯状穿孔2种或者仅具有单穿孔；导管间纹孔式为互列；导管－射线间纹孔式类型丰富，主要为刻痕状和大圆状。木射线有单列和多列射线2种类型，单列射线稀少、短，多列射线数量多；射线组织主要为异形Ⅲ和异形Ⅱ；轴向薄壁组织以傍管状为主，少数有带状或轮界状。油细胞和粘液细胞普遍存在于射线薄壁细胞或轴向薄壁细胞中。木纤维由韧性纤维和纤维管胞组成，部分树种具分隔纤维。

The first passage of YS-MSCs which grew in good condition was obtained, and adipogenic differentiation of YS-MSCs was induced by dexamethasone and insulin, and angioblast was induced by vascular endothelial growth factor and basic fibroblast growth factor.

取生长状态良好的第1代细胞，以地塞米松、胰岛素定向诱导卵黄囊间充质干细胞向脂肪细胞方向分化；以血管内皮生长因子和碱性成纤维细胞生长因子体外诱导其向血管内皮细胞方向分化。

In SMC group, relative stable of the both ends of fracture reduce unnessary repeated stimulation of injury, and at same time, axio-stress advantageous for preosteoblast and mesenchymal cell transmit to osteoblast and chondroblast. Material and design superiority of SMC form unique internal mechanical environment, and which benefit for bone healing. The study took deep investigation of SMC on fracture healing.

上述结果证实：SMC内固定可对骨折端施加主动的、持续的轴向应力刺激，形成沿长骨骨干轴向的成骨导向力值，配合环抱翼的把持作用，形成了区别于CP、IN的独特的稳定、有效的机械内环境，骨折端的相对稳定减少了不必要的损伤再刺激，同时持续轴向应力有利于成骨祖细胞、间充质细胞向成骨细胞和成软骨细胞转化、促进了成骨诱导过程的发生与发展，加快了骨折愈合的修复与重建。

RESULTS: On day 2 after PH, oval cells began to proliferate around the portal area, and matrilin-2 deposition was observed in the hepatic sinusoids in the periportal area. On day 9, proliferating oval cells were present in the hepatic acini, and matrilin-2 upregulation was noted. On day 12, as oval cells differentiated to form hepatocellular nodules, matrilin-2 was distributed mainly in the periphery of the nodules, and little protein was present in the nodules.

结果： 肝脏部分切除术(partial hepatectomy， PH)后第2天，卵圆细胞开始向门静脉周围区域增殖， Matrilin-2主要出现在门静脉周围的肝窦状隙内；术后第9天，卵圆细胞进一步向肝实质内增殖， Matrilin-2表达增加；术后第12天，随着卵圆细胞分化为小肝细胞结节，大多数Matrilin-2位于结节周边，少数出现在结节内。

The vein grafts with perivenous support by fibrin glue demonstrated a statistically decrease in neointimal and medial hyperplasia compared with non-supported vein grafts（p.01）. immunohistochemical analysis showed that the expression of proliferating cell nuclear antigen was inhibited significantly in fs group compared with ns group. almost intact endothelium was showed in fs grafts（p.01）. but in ns grafts there were severe endothelium damages where lymphocytes infiltrated. in ns grafts, the smooth muscle cells were hyperplasia and migrated to intimal, whereas in fs group, the hyperplasia of smooth muscle cells were inhibited and migrated to adventitia.

免疫组化结果显示与ns组相比，fs组移植静脉细胞增殖核抗原表达明显受到抑制；ns组内皮层损伤严重，有淋巴细胞侵润，而fs组内皮层则几乎完好无损（p.01）；在ns组平滑肌细胞增殖活跃且向内膜迁移，fs组平滑肌细胞增殖受到抑制，且向外膜迁移；在fs组移植静脉外膜有丰富的成熟微小血管网形成，但是在ns组，微小血管数量则非常少。

Results After restimulation with antigen, some of the CD4 +T cells were positive either for Th1 or Th2 cytokines, but few cells was positive for both of them.

Th1细胞产生IFN γ、TNF β和IL 2 ，参与细胞介导的免疫反应；而Th2细胞产生IL 4、IL 5、IL 10和IL 13等，参与体液介导的免疫反应；Th0细胞分泌混合型细胞因子，当受特异性抗原的刺激时，在微环境的影响下，可以向Th1方向转化或向Th2方向转化。

In order to know the evolutionary relationships between false vein, ISC, and VSC in Pteridaceae, 19 grnera and 82 species in Pteridaceae were sampled in this study; silica deposition types, ordinary epidermal cell morphology and veinal epidermal cells morphology of these species were surveyed. On the other hand, a convenient method was developed for this goal. In this method, a tabletop SEM with backscattered electron detector accompanied by post-cooling method was used.

为进一步探讨凤尾蕨科假脉、脉间矽异形细胞与脉上矽异形细胞之间的关系及其於凤尾蕨科内的演化，本论文选取19属82种的凤尾蕨科植物，观察其叶表面之二氧化矽堆积型式、一般表皮细胞形态以及脉上表皮细胞形态；同时，也开发出利用侦测背向散射电子之桌上型扫描式电子显微镜配合后冷却技术(post-cooling method)之快速检测法，该方法利用背向散射电子讯号强弱与平均原子数(average atomic number， Z number)之相关性得到矽异形细胞与一般表皮细胞具有明显对比之影像。

Some cell dropped into the cavity and became free. Thrombosis or part organization could be seen. The internal elastic layer became thin, disappear or broken. In internal and middle layer existed fibroblasts, fibrocytes and collagen. Some of the wall indicated hyaline change, soomth muscle cell decreased greatly. The massive inflammatory cells invaded the middle and external layer. There were many foam cells in the capsule tissue. Cytoplasm was filled with fatty tissue and cholesterol. some cavities were full of thrombosis. Some thrombosis was fibrosis, the bottom was organization. The surface of the thrombosis existed red blood cell and librae.(2)Elatic fibrila staining: the internal elastic menbrane almost completely disappeared, the intact internal elastic menbran could be seen in the new small vessels.

动脉瘤管壁厚薄明显不均，全层或局部区域显著变薄向外膨出，内皮细胞空泡变性或坏死脱落，部分内皮细胞剥离并突入管腔成游离状，可见血栓形成及部分血栓机化；内弹力板变薄、消失或突然中断；在内膜及中膜部位主要为纤维母细胞、纤维细胞和大片胶原；部分动脉瘤壁呈均质状玻璃样变，平滑肌细胞明显减少；中膜和外膜可见大量的炎性细胞浸润；瘤壁组织有纤维母细胞、纤维细胞、大片胶原成分及较多泡沫细胞，胞浆内充满脂类物质及胆固醇结晶；部分动脉瘤腔内充满血栓，有的血拴已经纤维化，血栓基部机化，血栓表面有红细胞和纤维素。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐