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The results of prediction agree well with the experimental ones. It proves that the thermo-mechanical analysis、the neural network models of flow stress and microstructure as well as the numerical simulation system developed combining coupled thermal-mechanical rigid-viscoplastic FEM with BP neural networks are reliable to describe the hot back extrusion process of Ti15-3 alloy truly. The simulation system has good prediction capacity for the microstructure evolution of Ti-15-3 alloy during hot backward extrusion and after solution treatment. It can be used to replace the experiments for predicting the flowing laws and the distribution of microstructure.

由此证明,本文的热力耦合理论分析、流变应力及显微组织的神经网络预测模型以及采用三维热力耦合刚粘塑性有限元与三层向后传播的人工神经网络相结合的方法开发的Ti-15-3合金筒形件热反挤成形的显微组织数值模拟系统是可靠的,能够真实地描述Ti-15-3合金热反挤工艺过程,并对其反挤成形及固溶处理后的显微组织具有很好的预测能力,可以被用来代替预测其反挤成形规律和组织分布情况的试验。

In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期,高度表达的合子转录本仍在所有的胚盘细胞中,但至90%下包期时,pou2的表达向胚胎背部的正中线汇聚,集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时,pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时,转录本消失,这与RT-PCR结果高度一致。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明:①在适宜的浓度体外培养条件下,NSCs能形成典型的神经干细胞克隆球,Vimentin免疫荧光染色阳性,单靠丝裂原刺激或NSCs自我调节和分化诱导,不会产生典型的终末分化的成熟神经细胞;②海马源性的NSCs维持干细胞特性的时间更长,生物学特性更优;③传至第2~3代的NSCs尚未分化时移植最佳;④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞;⑤各种子代克隆球和细胞存在广泛的突触联系;⑥NSCs与OECs联合培养时,不需丝裂原刺激即能形成克隆球,获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞;⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化,但并不出现终末分化;⑧本研究培养条件不利于成纤维细胞、O4生长。

The double labelling technique by combination of horseradish peroxidaseretrograde tracing with immunohistochemical staining for γ-amino-butyric acidwas used to observe the distribution of GABA-positive neurons in the abducens and vestibular nucleus,and its projection to oculomotor nucleus of rats.

用辣根过氧化物酶(horseradish peroxidase,HRP)逆行标记结合γ-氨基丁酸(γ-amino-butyric acid,GABA)的免疫组织化学双标技术观察大鼠的展神经核和前庭神经核内GABA阳性神经元的分布,以及其向动眼神经核的投射。

Currently autografting is the most common clinical treatment method, although it has some obvious disadvantages, for example, limited amount and length of nerve for grafting, disfunction of donor site, mismatching, misorientation regeneneration, etc.

目前临床上最常用的治疗是自体神经移植,但是它存在着明显的缺点,如可供移植的神经数量和长度有限,造成供区的功能障碍,大小难以匹配和可造成错向再生等。

The PRL-like cells were also presented in the ventral ependyma of the lateral ventricle and the glial lamina in the basal surface of the brain. The processes of PRL-LIR cells in PPN, SCN, PVN and PAN mainly projected to the third ventricle, those in AN and ELV mainly projected to the lateral ventricle, and those in SON to the glial lamina in the basal surface of the brain. The results showed that PRL could be released into ventricularis system and participate in the regulation of the cerebrum-cerebrospinal fluid circuit.

视前室旁核、视交叉上核、下丘脑室旁核、弓状核等核团内的PRL阳性神经元有突起向第三脑室投射,伏隔核内及侧脑室室管膜上的PRL阳性神经元有突起伸至侧脑室,视上核的PRL阳性神经元也有突起投射至脑基底神经胶质板,表明鸡脑内的PRL可以释放入脑室系统,参与调节脑-脑脊液神经体液回路。

Operative design: After gastric anterior wall was exposed, any Crow's nerve was identified from antral surface nearby gastric angle. Along the Crow's nerve, Latarjet nerve was found, gastric branches of Latarjet were gradually severed, the dissection proceeds to up towards the diaphrageal fissula, and then, the right gastroepiploic neurovascular bundle was cut, the distal two-thirds of the greater curvature was mobilized by dividing the greater omentum, the posterior nerve of Latarjet may be seen and isolated in the same way.

手术设计:暴露胃前壁,自胃角附近的胃窦壁表面任意找到一支&鸦爪&支迷走神经,随以此支为标志向上找到分出该鸦爪的Latarjet氏神经干,顺该神经向上游离,分离中避开向右走行的肝支及胃小弯的主要血管,逐一切断走向胃壁的迷走神经分支,直游离到膈肌食管裂孔平面。

The result showed that, the body wall most outer layer cuticle assumesthe translucent porodine, the surrounding entire body wall; Close isconnected with the cuticle 表皮层 for 合胞体 the structure, inthe back, the abdomen and its two sides separately adds thick to inprominent Cheng Jizhuang, separately contains the back side,腹侧the nerve and compares to the developed back, the abdomen nerve; Themyo- position to the body wall most inner layer, has the muscle cellto arrange becomes; Between the body wall and the digestive tractcavity is a body cavity; Digestive tract for 肌质 structure, afterinference for roundworm's esophagus.

结果显示,体壁最外层的角质层呈半透明的胶状,包围整个体壁;与角质层紧密相连的表皮层为为合胞体构造,在背、腹及其两个侧面分别加厚向内突出成脊状,分别包含背侧、腹侧神经和相比对发达的背、腹神经;肌层位于体壁最内层,有肌细胞排列而成;体壁与消化道之间的空腔为体腔;消化道为肌质结构,经推断为蛔虫的食道。

At this level, the upward projecting signals to higher-located centres and signals that eliciting spinal reflexes are subjected to complex modulatory effects coming from the descending fibers from supraspinal structures and propriospinal neurons through releasing some neuroactive substances, including neurotransmitters and neuromodulalors.

在这里,来自脊髓上结构的下行纤维和脊髓本身的局部神经元通过释放各种神经活性物质,包括神经递质和调质,对痛信号向高位中枢的上行传递和引起脊髓局部反射的过程,起着复杂的调制作用。

Results The length between posterior superior iliac spine and greater trochanter of femur was (15.2±1.35) cm for man and (11.5±0.72) cm for woman; the length between posterior superior iliac spine and ischial tuberosity was (13.3±0.87) cm in man and (10.3±0.49) cm in woman; the length between greater trochanter of femur and ischial tuberosity was (8.3±0.83) cm in man and (5.5±0.61) cm in woman; the surface projection where the superior gluteal artery, superior gluteal veins and superior gluteal nerve permeate the suprapitiform foramen was below the point 0~6.8 mm between 2/5 superior and middle trunk from posterior superior iliac spine to greater trochanter of femur; the surface projection where the inferior gluteal artery, inferior gluteal veins and inferior gluteal nerve permeate the infrapiriform foramen was at the point between 2/5 inferior and middle trunk from posterior superior iliac spine to ischial tuberosity; the surface projection where sciatic nerve permeate the infrapiriform foramen was at the point between 3/5 lateral and middle trunk from ischial tuberosity to greater trochanter of femur.

结果臀三角各边的长度分别为:髂后上棘转子间距,男(15.2±1.35) cm,女(11.5±0.72) cm;髂后上棘结节间距,男(13.3±0.87) cm,女(10.3±0.49) cm;转子结节间距,男(8.3±0.83) cm,女(5.5±0.61) cm。臀上动脉、静脉、神经出入梨状肌上孔的体表投影在髂后上棘与大转子连线中、上2/5交界处向下0~6.8 mm内;臀下动脉、静脉、神经出入梨状肌下孔处的体表投影在髂后上棘与坐骨结节连线中、下2/5处;坐骨神经出梨状肌下孔处的体表投影在坐骨结节与大转子连线中外3/5处。

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