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后鞭毛

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Basal bodies are the structures developed from bicentriole, the branched blepharoplast and blepharoplast, respectively, depending on the specific group of ferns concerned.

鞭毛、基体和多层结构中的微管带形成了游动精子三个独特的微管列阵,由于微管蛋白的后修饰作用这些微管列阵十分稳定;centrin是运动器中的重要成分,但功能尚不清楚,可能和细胞骨架及运动器的构建有关。

In addition, spermiogenesis includes chromatin condensation, migration of the cytoplasm and mitochondria to the caudal pole of the nucleus, formation of a moderate axial nuclear fossa containing the proximal centriole and part of the distal centriole and presence of a short cytoplasmic canal separating mitochondria from the flagellum.

另外,精子发生过程中还包括染色质浓缩,细胞质和线粒体向细胞核的尾端迁移,在核的后端中轴位置上形成中等大小的核后凹,近端中心粒和远端中心粒的一部分嵌在核后凹之内,短的胞质内陷管将线粒体与鞭毛分隔开。

In the process of spermiogenesis, the proximal and distal centrioles translocate to the rear of the spermatid nucleus where the proximal centriole is attached, while the distal centriole elaborates the flagellum. The fluorescent centrin protein was seen to be located in basal body of flagellum. But the fluorescent spots could not be detected in the epididymal mature spermatozoa.

在精子变态分化过程中,近端和远端中心粒向核后方移动,近端中心粒附在核后膜上,远端中心粒伸出鞭毛,荧光显示Centrin蛋白位于伸出鞭毛的基部,但在成熟精子中该蛋白消失。

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明:一定浓度的硒作用HP后,HP菌落数量随着硒浓度的增加而减少,菌落颜色由灰白色变为砖红色,溶血现象随硒浓度的增加和作用时间的延长而逐渐消失,HP的形态由弯曲形变为球形或椭圆形,鞭毛及菌毛出现脱落现象;硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显;一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素,颜色随硒浓度增加而加深;一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长,减少重组VacA表达量,但不影响其空泡毒活性及编码基因序列;纯化后的VacA重组蛋白可被VacA抗体识别,具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果:体外实验结果表明:一定浓度的硒作用HP后,HP菌落数量随着硒浓度的增加而减少,菌落颜色由灰白色变为砖红色,溶血现象随硒浓度的增加和作用时间的延长而逐渐消失,HP的形态由弯曲形变为球形或椭圆形,鞭毛及菌毛出现脱落现象;硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显;一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素,颜色随硒浓度增加而加深;一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长,减少重组VacA表达量,但不影响其空泡毒活性及编码基因序列;硒能增强重组VacA对胃癌细胞的抑制作用,使细胞分裂指数和集落形成率降低、S期细胞数量减少,G1期细胞总数增加;纯化后的VacA重组蛋白可被VacA抗体识别,具有良好的抗原性。

This last idea has not received much acceptance, since flagella lack DNA and do not show ultrastructural similarities to prokaryotes.

后一种想法没有被广泛接受,因为鞭毛缺乏DNA,在结构之外和原核生物也没有明显相似性。

Using special stains, one can see that some bacteria have attached to them wavy-looking "hairs" called flagella.Others have only one flagellum.

使用特殊的着色剂后,你会发现有的细菌上长着不少波状的"毛发"即鞭毛,而有的细菌只有一根鞭毛。

Transcriptome analyses showed that a substantial number of genes in the archaeon, including those involved in the methanogenesis pathway, were up-regulated after the contact with the flagellum protein.

转录组分析表明,古细菌中的大量基因,包括那些参与甲烷生成途径的基因,在其与鞭毛蛋白接触后都被进行了上调。

A flagellum propels the sperm, which may live in a woman's reproductive tract for two to three days after sexual intercourse, to the egg.

鞭毛约长50公忽,推动精子前进至卵子处,进入女性生殖道的精子在性交后可存活2~3天。

Results Approximately 700 protein spots were revealed in equivalent proteins of the promastigotes and axenic amastigotes separated by 2-DE,among which more than 90% protein spots showed equivalent quantity and distribution,with 6 proteins up-regulated and 3 proteins down-regulated in axenic amastigotes compared with promastigotes.

结果 等量的前鞭毛体与纯培养的无鞭毛体总蛋白经双向电泳分离后均可获近700个蛋白点,其中超过90%的蛋白点的分布和相对强度基本一致。

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