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After changing medium, BMSCs were incubated in serum-free DMEM containing BRA and dimethyl sulphoxide for 5 hours to 6 days. MAIN OUTCOME MEASURES: Cell transfection was observed with a fluorescence microscope.

主要观察指标:荧光显微镜观察细胞转染情况,免疫细胞化学染色检测转染细胞诱导后神经元表面抗原的表达。

A craniotomy with the size of 4 cm × 5 cm was placed at the site which corresponds to the sylvian fissure, the circular sulcus was exposed allowing transcortial exposure of the entire ventricular system, thus achieving the functional hemispherectomy.

手术均采用侧裂表面4 cm × 5 cm骨窗开颅,分开侧裂后经皮质进入侧脑室,完成功能性大脑半球切除手术。

Impression cytology and tear secretion was performed at different check-point time postoperatively after fresh amniotic membrane transplantation for conjunctival surface reconstruction after pterygium excision,conjunctival excision and symblepharon lysis.

选择临床连续病例共29例。其中复发翼状胬肉8例8只眼,蚕蚀性角膜溃疡8例8只眼,轻中度睑球粘连13例13只眼。按常规方法行新鲜羊膜移植,移植后不同时间复诊时进行羊膜表面的印迹细胞学检查,手术前后进行泪液分泌量的测定。

These results indiacated that tranferring macrophages combinedwith cytokines could be a new adoptive immunotherapy protocol foradvanced cancer,and rTNF used in vivo locally could induce andactivate macrophages to kill tumor cells.Monocytes when activatedunderwent a series of phenotypic and functional changes includingthe expression of IL-2R which may provide an important immunoregu-latory pathway.The presence of lectin-like molecules on thesurface of monocytes and tumor cells may bring theeffector/target cells together,thus facilitating the inductionof apoptosis in target cells by triggering the production ofcytolytic factors and the modification of target of targer cell surface anti-gens.(such as HLA-DR).

综合以上结果可以得出以下结论:转输巨噬细胞并结合细胞因子的联合应用是肿瘤继承性免疫中的又一新的、有潜力的方法;抗肿瘤细胞因子rTNF可通过激活巨噬细胞在体内抑制对其不敏感的肿瘤细胞的生长;人单核细胞在一定条件下激活后,可表达IL-2R,进而一方面增强其自身对IL-2的敏感性,另一方面在体内也具有调节T细胞功能的作用;单核细胞和肿瘤细胞上存在的凝集素类受体可促使两种细胞之间在凝集素介导下相互接近,并诱发单核细胞产生细胞毒因子,以及通过调节靶细胞上表面抗原的表达,促进诱导靶细胞的细胞凋亡。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术,分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因,以中国株HBV基因序列为依据,设计特异性多聚酶链反应引物,分别自43例、51例慢性HBV感染患者血清中扩增出目的片段,构建质粒Teasy-BCP和Teasy-pre S2,酶切鉴定后,采用聚丙烯酰胺凝胶电泳技术展示缺失突变,再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

Methods Accumulative doses of X-ray radiation emitted from the surface of terminal screen and radiation protective screen were measured by highly sensitive LiF thermoluminescent dosimetry.

方法采用高灵敏Lif热释光剂量计对计算机显示器表面及附加防护屏后的X线辐射剂量进行累积剂量监测。

Upon thrombogenic and inflammatory challenges, P-selectin is rapidly expressed, by exocytosis, on activated platelets and stimulated endothelial cells.

当细胞受到凝血酶原或炎症因子刺激后,P-选凝素会迅速地通过外排表达在活化的血小板和内皮细胞的表面。

Three months later, the surface of the articular was smoother than the sham-operated side. The histological score of cartilage was significantly higher than the sham-operated side in patellofemoral joint group p<0.05=, in the tibiofemoral group the difference was not obvious.

处理后3月,发现髌股关节组双极射频治疗侧髌骨软骨表面仍光滑,软骨组织评分高于对照侧(p<0.05);胫股关节组双极射频治疗侧股骨内髁软骨面的形态比对照侧平整,软骨组织学评分没有显著差异。

Uniform PCB surface without residue can be obtained after tinning

过锡后PCB表面平整均匀、无残留物

With titanite as the surface modification agent of sericite, the effects of the dosage of modification agent, massic ratio of midium/sericite, density of slurry, grinding time, temperature and pH value on the surface modification were analyzed. The properties of polypropylene filled with the moth- fled filler were tested.

采用钛酸酯偶联剂作为表面改性剂研究了改性剂用量、介质物料比、磨矿浓度、改性时间以及温度和pH值对改性效果的影响,并对最终改性产品填充聚丙烯后的复合材料进行了性能测试。

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推荐网络例句

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里,举止就文雅一些,因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计,这件高贵的制服是香港本土制造,是我们第一套不配帽子的制服。

Do not 'inflate' your achievements and/or qualifications or skills .

不要 '夸大' 你的业绩或成果,条件或者技能。