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Methods: Forty mice were divided into four groups randomly:normal group,model group,ligation group and treatment group.The model was made by the method of partly ligating the recta outside the body.The level of endotoxin in blood plasma was measured by dynamic nephelometric method and the content of TNF-a in blood serum was detected by the method of radioimmunity.(2)After the mice were killed,their left lungs were taken out for bronchi-alveoli fluid, then the content of MDA in BALF was tested by TBA method.(3)The vitality of PAM in BALF was countied on the counting set of erythrocyte,and the blue-dyed cellswere counted, then the percentage of PMN was worked out.(4)The pathological features of the mice\'s lung tissue were observed with routine and ultramicro pathological mechanism.

①将40只大鼠随机等分为正常组、模型组、解扎组、治疗组,以体外直肠不全结扎法造模,以动态浊度法检测各组大鼠血浆内毒素水平,放射免疫分析法检测各组血清TNF-α含量;②大鼠处死后,取左肺进行支气管肺泡灌洗,以TBA法测定支气管肺泡灌洗液中丙二醛含量、白蛋白含量及肺表面活性物质水平;③在红细胞计数盘上作BALF中肺泡巨噬细胞活力计数,计数兰染的细胞数,求出死亡百分率;在光镜下计数BALF沉渣200个细胞,计算中性粒细胞所占百分比。

In this paper, we systematically study the surface nanocrystalline treatment process and low temperature nitriding process of steels.

本文主要对纯铁和45钢交流电表面纳米化处理以及预处理后的低温渗氮工艺进行了系统研究。

MHC class Ⅱ,CD40,CD80,and CD86 on DC were measured using flow cytometry.Results The ability of DC to stimulate nonadherent splenocytes proliferation was decreased after injury,and the levels of cytokines were changed.

致伤后24小时分离小鼠脾脏DC ,检测脾脏DC在体外抗原作用下刺激脾非黏附细胞增殖的能力、细胞因子分泌功能及其表面重要分子表达的情况。

In many cases, dental implants should apply in combination with onlay bone grafts.

大多数人认为,传统应用髂骨表面贴附移植后吸收严重,影响重建效果。

The neutralizing activity of these antibodies and IgG fractions is acquired through post-translational modifications, through opsonization of virus particles into macrophages and immature dendritic cells, or through expression of antibodies on the surface of HIV-1-susceptible cells.

这些抗体或IgG片段的中和活性可以通过抗体分子翻译后的修饰,或介导巨噬细胞和未成熟树突状细胞的对病毒颗粒的调理作用,或者通过表达在HIV易感细胞表面来体现。

The toxicity and the osteogenous ability on the surface of PCHA: the toxicity was examined by coculture with PCHA to record growth of cells with MTT method. The osteogenous ability was investigated by observation of the formation and growth of cells on the surface of PCHA bone cement.

PCHA毒性检测和表面细胞活性的观察:将PCHA浸出液与骨髓基质细胞共同培养,通过MTT比色描记细胞生长曲线,快速判定制孔剂添加后PCHA的毒性反应;将PCHA制成标准试件,与骨髓基质细胞共同培养,观察细胞形态和生长活性。

The biological features of hBMSCs and cryogentic storaged cells were analyzed.hBMSCs memberane antigens were identified by immunofluorescence staining.The potential osteogenous differentiation of hBMSCs was evaluated by the osteogeneic inducer kit.

倒置光学显微镜下观察细胞形态及生长情况,细胞计数法绘制细胞生长曲线,并分析冻存细胞复苏后的生长特性,免疫荧光染色测定hBMSCs表面标志,成骨诱导试剂盒检测hBMSCs向成骨分化的潜能。

MAIN OUTCOME MEASURES: Morphological changes in primary cultured and subcultured mouse BMSCs were measured. BMSC surface antigen of the second passage of mice (CD105, CD44, CD25, CD34) were determined using flow cytometry. The modified method was assessed to harvest the purity of stem cells. Activity of mouse BMSCs was identified using adipogenic and osteoplastic differentiation. The strength of fluorescent cells following multiple passage was observed.

主要观察指标:原代及传代小鼠骨髓间充质干细胞形态变化;对第2代小鼠骨髓间充质干细胞表面抗原,CD105,CD44,CD25,CD34进行流式细胞仪检测,评价改良方法获取干细胞纯度;通过成脂成骨分化,鉴定小鼠骨髓间充质干细胞的活性;观察多次传代后荧光细胞强度。

Some aggregates of the active elements appeared on the oxidic catalyst samples and the aggregates could be re-dispersed by sulfidation. The reduction and sulfidation ability of the regeneration catalysts were increased in low temperature region.

对催化剂氧化态和硫化态的表征结果表明,催化剂在失活—再生过程中,比表面等物理性质有一定的变化,再生催化剂活性组分发生聚集,该聚集体经硫化后可得到重新分散,催化剂的低温还原性和硫化性能增强。

In this paper, the carbon nanotubes made by CVD were purified by acid solution. Results indicate that the ports of CNTs were oxidated and opened up, the surface of CNTs bring a lot of oxygenous functions, which was useful for dispersion of CNTs in the water solutions.

采用液相氧化法对CVD法制备的碳纳米管进行了提纯,结果表明,经过提纯后碳管的端口被打开且表面产生了大量的含氧官能团,使其在水溶液中的分散性得到了改善。

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