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Using the immunohistochemical technique, nerve cell bodies and fibres immunoreactive to the anti-Met-enkephalin sera were observed in the cerebral ganglia, pedal ganglia, visceral ganglia, inspect ganglia and coat ganglia of Octopus ocellatus. Met-ENK immunoreactivity was also localized in the mouth, esophagus, craw, stomach, stomachic caecus, the epithelium of the intestines, rectum, sialoid gland, liver, branchia, branchia gland, ventricle, branchial heart, kidneys, ovary, fallopian tube, back of mantle, abdomen of mantle, wrist, membrane of wrist, funnel, and immunocytes of Octopus ocellatus.

免疫组织化学定位结果表明:(1)在短蛸的脑神经节、足神经节、脏神经节、视神经节、外套神经节、口球、食道、嗉囊、胃、胃盲囊、肠、直肠、前唾液腺、后唾液腺、肝胰脏、鳃、鳃腺、心室、鳃心、肾静脉腺质附属物、卵巢、输卵管腺、外套膜背面、外套膜腹面、腕、腕间膜、漏斗中检测到呈不同阳性反应的Met-ENK分子。

Results:(1)Among 40 rats, 36 rats were successfully established and the rate of success is 90 percent;(2)All the successfully established models demonstrated polydipsia, polyuria and the body weight was not increased. 6 rats suffered cataract after 3 months, and 4 rats died in 6 months;(3)There was an approximately 61% loss of retinal ganglion cells in the central retina and the thickness of retina thinner under microscope ( P 0.01 ).(4) Electroscope changes include the thinner of retina, disorganization of the membranous disc of the rod cells and the thickness of basal membrane of vessal.(5)In normal group, 1 month dibetes mellitus and 1 month treatmen group, there was no expression of ERK1/ERK2 on the retina tissues. In 3 month diabetes mellitus group, the expression of ERK1/ERK2 was positive.

结果:①40只建模大鼠中36只建模成功,建模成功率为90%;②建模成功的大鼠都表现出多饮、多尿、消瘦、体重不增的表现,有6只大鼠在3个月后出现白内障,有4只大鼠在喂养接近至6个月时死亡;③HE染色光镜下6个月大鼠后极部视网膜节细胞层细胞数明显减少,减少约61%(P.01),后极部视网膜明显变薄(P.01);④电镜观察,视网膜变薄,视杆细胞膜盘紊乱,血管基底膜增厚等表现;⑤正常组、糖尿病组和治疗组1个月大鼠视网膜中未见ERK1/ERK2的表达,糖尿病组视网膜组织中3个月时可见少量表达,ERK1/ERK2表达部位为神经节细胞层和内核层;6个月时表达强阳性,部位表达不仅见于内核层、神经节细胞层,色素上皮层也见表达。

A19 line poem of fixed form consisting of five tercets and a final quatrain on two rhymes , with the first and third of the first tercet repeated alternately as a refrain closing the succeeding stanzas and joined as the final couplet of the quatrain .

田园诗一种有固定格式的19诗;由五个三押韵的诗节和一个四诗节组成,其四诗节有二个韵,在第一个三押韵诗节中,第一和第三作为结束后继诗节的重复句间隔地重复出现,并作为四诗的结尾对句一起再现

8 A foul by either team:* Before the beginning of a period other than the first period, or * During the alternating possession throw-in, Does not cause the throw-in team to lose that alternating possession throw-in.

比如跳球后是B队拿到了球,第一节没有交替发生,那第二节本该A队发球。虽然A队在之间发生技术犯规,但是这个犯规不能使A队失去第二节开始的球权,仍然应该是B队2罚后由A队开球。

It showed that for each type of P and N cell the contralateral member of the pair evoked larger synaptic potentials in the AP cell that did its ipsilateral homologue and the P and N cell with a ventrally situated receptive field produced a larger synaptic input on the AP cell that its counterpart with a dorsally situated receptive field.

比较P和N细胞在AP细胞上的突触后电位幅度,可见对侧P和N细胞在邻节的AP细胞的突触后效应强于同侧的P和N细胞在AP细胞上的突触后效应;具有腹部感受野的P和N细胞在邻节AP细胞上的突触后效应强于具有背部感受野的P和N细胞在AP细胞上的效应。

Two level cervical discectomy and fusion can increase stress in the caudad (C5-6, C6-7) zygapophyseal joint, which may be one of the causes for adjacent cervical spondylosis postoperatively.

颈椎双节段椎间盘摘除融合内固定后邻近下位关节突关节压力增加,可能是多节段颈椎融合后邻近节段发生退行性变或退行性变加速的原因之一。

Objective to evaluate the functional recovery of acute spinal cord injuried rats treated with exogenous wnt-3a signal protein administration and to explore its mechanism.methods moderate spinal cord contusion injury was made in 40 adult sprague dawley rats at t10.twenty rats served as contusion controls(group 1).twenty rats were treated with wnt-3a for three days after injury (group 2).the functional recovery of the rats was observed through basso,beattie,bresnahan open field locomotor score.rats were killed at 14 or 28 days after injury,then spinal cords were removed for histopathological examinations,and the expression of the bromodeoxyuridine plus neural cell markers was stained with immunohistochemical method.results rats of two groups receiving a contusive injury recovered substantial function within 1 week.by 28 days,rats in groups 2 scored 7.0 points better on the bbb scores than rats in group 1 group 2=16.94,after 28 days vs.

目的 研究外源性wnt-3a信号蛋白对脊髓损伤的修复作用,并探讨其作用机制。方法取40只成年雌性sd大鼠在t10节段制备适度脊髓挫伤模型。随机从中取20只为损伤对照组(group 1),另外20只为损伤治疗组(group 2)。脊髓损伤3天后用wnt-3a蛋白治疗。这些大鼠的功能恢复通过basso、beattie、bresnahan开放视野运动评分来观察。这些大鼠分批在损伤后14天或28天被处死,取出损伤节段脊髓用来组织病理学检查,同时用5-溴脱氧尿嘧啶核苷和神经细胞标记物进行免疫组化染色。结果两组大鼠在伤后一周运动功能有明显的恢复。不过,到损伤后28天,我们观察发现,损伤治疗组中的大鼠bbb评分比损伤对照组中的评分高出7.0分左右(group 2∶16.94±1.18,group 1∶9.89±1.29;p.05),光镜和电镜检查发现wnt-3a蛋白对髓鞘形成和轴突再生有一定的修复效果。

Result: Porosis and inflammatory cell infiltration in the spinal cord, rupture of muscle bundles, hypertrophia and over growth of muscle cells and collagens, and inflammatory cell infiltration in the bladder wall could been seen under HE dyeing. Nissal dyeing showed the decrease of neurons at the injured segment, upper and lower segments of the spinal cord. Result of immunohistochemistry showed there were caspase-3 positive expression in all the segments. The injured spinal cord and bladder expressed significantly more caspase-3 positive results (P.0l), upper and lower segments were lower than the injured segments (P.01), but higher than the control team (P.0l).

结果:HE染色发现脊髓损伤后脊髓内空洞形成、炎性细胞浸润等病理学变化,膀胱壁中可见到肌束断裂、肌细胞肥大增生、胶原增生、炎性细胞浸润等病理学改变;Nissal染色显示脊髓损伤后损伤节段及其上、下节段均出现神经元数量的减少;免疫组化结果表明3组均有不同程度的caspase-3表达,损伤组脊髓和膀胱内膜caspase-3表达较正常组显著增加(P.01),损伤上段及下段caspase-3表达较损伤节段为少(P.01),但均高于正常对照组(P.01)。

An essentialized and remarkably delimited Indian theatre consisting of just one genre, namely, the ancient Sanskrit theatre, with no space for the Ram Lila or other flourishing vernacular genres;(2) Histories from the nationalist period portray Indian theatre as primarily, though not exclusively, madeup of India's Western-influenced "modern" theatre genre but relegate the Ram Lila to the realm of unsophisticated folk theatre; and (3) Postcolonial-era histories offer a broad-based and plural Indian theatre encompassing all major genres, including the Ram Lila, but valorize postcolonial theatre withuntenable and extravagant claims.

我会在结论论证这些研究的源起,并列举三个现代印度文化和历史的不同阶段作为例证:(1)东方主义时期的印度剧场史,呈现出一个本质主义、明显画地自限的印度剧场,当中只有一个剧种:古老的梵文剧场,毫无肚量容纳罗魔节戏或其他盛极一时的地方戏剧;(2)国家主义时期的剧场史,此时最主要由深受西方文化影响的印度「现代」剧场构成,罗魔节戏被贬抑为难登大雅的地方小戏;(3)后殖民时期的剧场史,提供了一个宽广、多元,兼容并蓄的印度剧场,罗魔节戏也在其中,但此时期过於夸大了后殖民剧场的资产。

Pumpkins are also a symbol of Halloween46; The pumpkin is an orange-colored squash, and orange has become the other traditional Halloween color46; Carving pumpkins into jack- o'-lanterns is a Halloween custom also dating back to Ireland46; A legend grew up about a man named Jack who was so stingy that he was not allowed into heaven when he died, because he was a miser46; He couldn't enter hell either because he had played jokes on the devil46; As a result, Jack had to walk on the earth with his lantern until Judgement Day46; So Jack and his lantern became the symbol of a lost or damned soul46; To scare these souls away on Halloween, the Irish people carved scary faces out of turnips, beets or potatoes representing "Jack of the Lantern," or Jack-o-lantern46; When the Irish brought their customs to the United States, they carved faces on pumpkins because in the autumn they were more plentiful than turnips46; Today jack-o-lanterns in the windows of a house on Halloween night let costumed children know that there are goodies waiting if they knock and say "Trick or Treat!"

南瓜也是万圣节的标志性象征。南瓜是橘黄色的,所以橘黄色也成了传统的万圣节颜色。用南瓜雕制南瓜灯也是一个万圣节传统,其历史也可追溯到爱尔兰。传说有一个名叫杰克的人非常吝啬,因而死后不能进入天堂,而且因为他取笑魔鬼也不能进入地狱,所以,他只能提着灯笼四处游荡,直到审判日那天。于是,杰克和南瓜灯便成了被诅咒的游魂的象征。人们为了在万圣节前夜吓走这些游魂,便用芜菁、甜菜或马铃薯雕刻成可怕的面孔来代表提着灯笼的杰克,这就是南瓜灯(Jack-o'-lantern)的由来。爱尔兰人迁到美国后,便开始用南瓜来进行雕刻,因为在美国秋天的时候南瓜比芜菁更充足。现在,如果在万圣节的晚上人们在窗户上挂上南瓜灯就表明那些穿着万圣节服装的人可以来敲门捣鬼要糖果。

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What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢?

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我,一个心中布满虚荣的女孩子真实的想法。

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哦,我还给我自己买了一个新笔记本"他说。"