后肾

The color Doppler ultrasound examination is conductive for diagnosis of oliguresis or anuresis.A good curative effect could be achieved using active waiting therapy in the treatment of oliguresis or anuresis.

彩色多普勒超声对诊断移植肾后少尿或无尿有较大帮助，采用此法治疗移植肾术后少尿或无尿取得较好效果。

Results:1 case with complicated pyonephrosis was treated with puncture and canalization;Two-stage operations of mini-PCNL was treated 1 week later.20 cases underwent one-stage PCNL,and were treated with single-access PCNL.The total stone clearance rate in one-stage PCNL was 95.2%;residual stones were seen in 5 case(23.8%),Only 1 case had obviously bleeding in or after operation.The mean time of operation was 2 hours,average hospitalization was 12 days.All were followed for 3-12 minths,1 case had recurrent stones.

结果：21例患者中，并发肾积脓1例，先行经皮肾穿刺造瘘术，1周后行二期mini-PCNL，其余20例均行一期穿刺取石，均为单通道穿刺取石，一期结石清除率95.2%；其中术后5例有残余结石，残余结石发生率为23.8%；术中术后有明显出血1例；手术平均时间2小时；平均住院时间12天；随访3～12个月，1例结石复发。

The deduced amino acid sequence of red sea bream MHC classⅡB 1(Chma-DAB*0101) had 87.1%,85.1%,87.1%,90.4%,87.1%,90.8% identity with those of red sea bream classⅡB 2, 3, 4, 5, 6, 7(Chma-DAB*0201-Chma-DAB*0701), respectively, and had 75.2%, 74.5%, 55.9%, 55.1%, 34.3% and 30.4 % identity with those of striped sea bass, cichlid, rainbow trout, Atlantic salmon, mouse and human, respectively.

以正常鱼和鳗弧菌感染后真鲷组织为材料，以反转录合成的第一链cDNA为模板，应用RT-PCR半定量检测方法对真鲷的MHCⅡB基因组织表达特点进行了研究，结果表明，在正常真鲷组织中，其中较强的表达于鳃、脾、头肾、肾、胃、小肠和心脏中，在肌肉和血中表达最弱。

Tumor excision was performed using ultrasonic shears, maintaining an adequate parenchymal margin. Bleeding from the tumor bed was controlled by bipolar diathermy . The renal artery was occluded when necessary. The edges of the renal parenchyma were approximately with 4 to 6, 2-zero absorbable sutures over hemostatic mesh.

超声刀沿瘤体边缘外0.5厘米处分离，由浅入深，边切割边止血，直到完全切除肿瘤，如切除肿瘤过程中肾脏创面出血过多影响操作，可暂时阻断肾动脉并计时，迅速切除肿瘤，双极电凝止血后，即可开放肾动脉。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

Acute tubular injury is commonly observed in renal allografts, especially early after transplantation.

急性肾小管损伤经常发生在移植肾，尤其是肾移植后早期。

In addition to the common effect of supplementing qi, YiQiYangYin Fang has unique clinical application in exercise-induced fatigue disease because of its function of reinforcing yin; YiQiBuShen Fang is used for treating senescence diseases by reason of its function of invigorating kidney.

本课题以中医药理论为指导，依据益气养阴方和益气补肾方的功效与应用，结合目前运动性疲劳和衰老的产生机制等方面的研究进展，采用现代药理学研究方法，观察益气养阴方和益气补肾方对环磷酰胺致小鼠免疫抑制的抵抗作用及对小鼠常压耐缺氧和断头后张口喘气时间的抗应激能力，同时分别采用运动性疲劳大鼠模型和自然衰老大鼠模型进一步探讨益气养阴方抗运动性疲劳机制及益气补肾方抗衰老机制。

According to the principles of classification of urethral damages, the urinary fistulae after kidney transplantation was classified in terms of the cause, location and extent of them.

文章根据尿道损伤疾病分类的原理，将肾移植后尿瘘分别按照尿瘘病因、尿瘘部位、病变程度等进行分类；讨论了肾移植后尿瘘经久不愈的原因以及诊断时应注意的几个问题。

According to the principles of classification of urethral damages, the urinary fistulae after kidney transplantation was classified in terms of the cause, location and extent of them. Moreover, why it was difficult to cure urinary fistulae was discussed, and the key points in the diagnosis were illuminated.

文章根据尿道损伤疾病分类的原理，将肾移植后尿瘘分别按照尿瘘病因、尿瘘部位、病变程度等进行分类；讨论了肾移植后尿瘘经久不愈的原因以及诊断时应注意的几个问题。

To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.

为了解猪皮肤成纤维细胞PERV在体外和体内的感染性，通过建立猪皮肤成纤维细胞系，将所建细胞系与人胚胎肾293细胞体外共培养，并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验，结果表明，猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中，猪内源性逆转录病霉感染人胚胎肾细胞，进一步证实和拓宽了猪细胞PERV感染人细胞的范畴；猪皮肤成纤维细胞移植SCID鼠皮下后，导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官，但是并未检测出SCID鼠组织中表达PERV env RNA。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。