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Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA,用PCR扩增出BP26基因,并克隆到pMD18-T载体上,测序正确后,再亚克隆至pET-28a载体上,转化到大肠杆菌BL21(DE3)中,用IPTG诱导表达,经组氨酸结合树脂柱纯化,并对纯化后的表达产物进行Western blot鉴定。

An electric skin abrader was used to smooth the superficial scars in different positions. The wounds were treated locally with MEBO. Result: 108 patients with superficial scars received abrasion and MEBO treatment.

使用韩国产高速电动磨皮机,对不同部位皮肤表浅瘢痕进行磨削后,再应用MEBO湿润暴露疗法处理磨皮后的创面,观察创面愈合情况。

The water quality of the sewage aftertreatment by membrane technology was improved greatly.

二级处理后的废水经过上述膜工艺深度处理后,各项指标大大改善。

The research contents mainly include: First, the influence of foundation constraint condition to the temperature stress is analyzed, the temperature stress formula of ultra-long concrete anchor plate is deduced, then the influence of length to structure crack can be gotten from the formula. Second, a breathing method is set up to solve the problem of temperature stress in ultra-long concrete constructions. By setting the sliding floor, a breathing system is built up and the temperature stress reduced distinctly. Third, the effect of prestress technique in the control of temperature stress is analyzed, it is simulated and calculated through FEA to get the right way to apply the prestress. Forth, the delay of temperature field and unsteady conduction is considered, the temperature field of ultra-long concrete structure with later poured band is simulated, then temperature stress distribution after casting the concrete is calculated, finally the effect of later poured band to temperature stress of ultra-long structure is analyzed.

本文的研究内容主要包括:(1)分析了基础约束对超长混凝土结构温度应力的影响,推导了超长混凝土地基板温度应力公式,从而得出长度对结构裂缝的影响;(2)针对超长混凝土裂缝问题,提出了一种可呼吸的解决方法,通过设置滑动层,使结构成为一种"可呼吸"体系,从而有效的降低温度应力;(3)分析了预应力技术在控制超长结构温度应力中的作用,通过有限元模拟计算,得到合理的预应力施加方式;(4)考虑温度场的滞后效应及不稳定传导,对带后浇带超长混凝土结构浇注后的温度场仿真模拟,计算浇注后的温度应力分布,分析后浇带对超长结构温度应力的影响。

Because harden steel be difficult to machine. but when anneal , It can be easily machine .

因为淬火后的钢很难进行加工,但退火后则很容易加工。

The average percent content of saturated hydrocarbons and aromatic hydrocarbons is changed from 56.32% to 60.38% and from 21.44% to 22.97% respectively, and resins compounds are increased by 2.09%, and asphaltum is reduced to 1.29%.

原油经配伍菌作用后含蜡质量分数平均降低17.56%,含胶质量分数平均降低26.54%,处理后的原油表观黏度与处理前相比降低50%,原油流动性得到改善。

Genders were compared with regard to the extent of coronary atheroma at baseline and subsequent change in response to use of established medical therapies.

在给予标准药物治疗后,比较不同性别患者冠脉粥样硬化斑块在治疗基线及治疗后的变化情况。

When bilateral ureters were completely obstructed and severe azotemia occurred, the prognosis may be better and the renal function can be recovered if the obstruction is removed within 48 hours.

当输尿管完全梗阻并出现重度氮质血症后在实施梗阻后的第二个24h内解除梗阻,可有效恢复肾功能,预后较好;若≥72h解除梗阻,则肾功能无法恢复,预后较差。

To compare the temperature diversity by collecting and analysing these infrared thermogram data. Results: While the operator just put their hands to the back of the quizzee for 3 minutes, the temperature of Xinshu and Dazhu rised and then descent gradually; But the temperature rised all the same about another 3 minutes while the operater using the vibrating manipulation; The temperature of Laogong and Zhongchong rised gradually, especially in 120 seconds and 90 seconds after using the vibrating manipulation.

结果:三组操作者在空置后3min,心俞穴和大杼穴处温度均出现变化,且与操作前温度有显著差别(P.05);在空置3min后,被操作者的心俞处以及大杼处的温度呈自然下降趋势;而在行掌振法时,被操作者心俞处以及大杼处的温度变化,不仅发生在3min后,而且可延续到振法停止后的1min到3min内,与操作前温度相比,有显著差别(P.05);在空振中,高年资医师的劳宫处和中冲处温度呈现逐渐上升趋势,在振法进行到90s时,中冲处温度出现显著变化,与开始温度相比,有显著差别(P.05),而劳宫处的温度则是在120s时,出现显著变化,与开始温度相比,有显著差别(P.05)。

The Go moved backward with 1.35mm and upward with 1.90mm in group I, the gonial angle increased for 3.69°, and angle between palatal plane and mandibular plane was increased for 5.00°; In group II, the Go moved backward with 1.85mm and upward with 3.32mm , the gonial angle increased for 4.06°, and angle between palatal plane and mandibular plane was increased for 5.22°. Both in group I and II, there was a tread for clockwise rotation of mandibular corpus. The length of pterygomasseteric sling was mild increased due to the backward movement of Go.

在Go点的变动方面,在Me点向前位移的族群里,术后Go点平均向后1.35mm,向上1.90mm, gonial angle 平均增大3.69度,腭平面与下颚平面的夹角则是增加5.00度;在Me点向后位移的族群里,术后Go点平均向后1.85mm,向上3.32mm, gonial angle 平均增大4.06度,腭平面与下颚平面的夹角则是增加5.22度,不论是向前或是向后的族群,结果皆显示翼咀嚼肌悬吊带有轻微被拉长的迹象。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?