后的

We studied an additional 7 rabbits after left entricular endocardial cryoablation, resulting in a thin layer of suriing epicardium. Cai sinkholes occurred 31±12 ms after the shock, followed in 19±7 ms by first postshock actiation in 63 episodes of unsuccessful defibrillation.

我们进一步研究了左室内膜冷冻消融后的7只兔，Cai穴发生在电击后31±12ms，在63次除颤未成功情况下，首次电击后激活出现在电击后19±7ms的Cai穴。

Results: 48 h after treatment, most of the patients had a marked improvement in irf and ret (p＜0.05), and irf showed a more significant increase. moreover, the value before treatment and 48 h after treatment of irf had no overlap; irf and ret were further increased after 1 week; after 12 weeks irf%, hct, ret, rbc and hb were significantly increased than before treatment, bone marrow erythrocytic series proliferation was more active than that before treatment, each kind of erythroblast ratio and the percentage of total erythrocytic series were significantly increased, especially late erythroblasts proliferation.

结果：治疗过程中大部分患者在epo注射48 h后irf、ret升高（p.05），irf升高更为显著，且irf在给药前和给药48 h后的数值无重叠区，1周后irf、ret进一步升高，12周时irf、hct、ret、rbc、hb均比用药前显著增高，骨髓红系增生较治疗前明显活跃，各类幼红细胞比率及总红系所占百分数均显著升高，其中以晚幼红细胞增生最为明显。

Predeterminer):all,both,half;double,twice, three times,etc;one-third,two-thirds,etc;what,such, etc 中位限定词:the,a,zero;this, that,these,those;my,your,etc:John's,the old man's,etc; some,any,no,every,each,either,neither,enough,what, which,whose,etc 后位限定词:one,two,etc;first,second,etc;next,last,other,etc; many,much, few,little,fewer,fewest,less,least,more,most;several,plenty o f,a lot of,lots of, a great number of,a greatdeal of,a largeamount of;such,etc

在三类限定词中，中位限定词是最关键的。所谓前位限定词是指那些可以位于中位限定词之前的限定词，所谓后位限定词是指那些可以位于中位限定词之后的限定词。例如： all the four teachers 所有这四位教师前中后上述限定词的位置是固定的，不能掉换，就是说它们必须按"前位—中位—后位"的顺序排列。以此类推，如果出现两个限定词，则只能按前位—中位，前位—后位或中位—后位的顺序排列。

3Chondrogenesis was assessed using Alcian blue staining, Toluidine bluestaining, Safranin O/Fast Green staining at 7 and 14 days, still collagenⅡimmunohistochemistry and aggrecan immunofluorescence at 4, 7 and14 days after initial chondrogenic induction of adipose -derivedmesenchymal stem cells.

3观察脂肪间充质干细胞在诱导后的生长增殖情况、形态变化，于诱导7、14天后应用阿尔新兰染色、甲苯胺兰染色、藩红0／固绿染色，以及4、7、14天后应用Ⅱ型胶原蛋白免疫细胞化学、aggrecan免疫荧光评价其成软骨能力。

He strongly emphasized that he arrnaged this on purpose; He frankly said that he's too childish in relationships, after breaking up with Patty Hou the feeling of singlehood will still get lonely, and asked the media to wait until he's mature at thirty, to then discuss about relationship issue.

周董」周杰伦情变后瘦了3公斤，昨天他为新专辑「依然范特西」所受批评提出反驳，针对网友批他没进步、太多歌有旧影子，他强调是故意安排；感情上他坦承太幼稚，和侯佩岑分手后的单身滋味还是会寂寞，请媒体等他3 0岁成熟后，再讨论感情问题。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Results The peak of antibacterial activity appeared at 36-48 hafter induced by four inducements (Because many M.domestica larvae pupated,we did not observe the peak of antibacterial activity by heat-shock). The antibacterial activity of antibacterial proteins by S.aureus was best,and the antibacterial activity of antibacterial proteins by heat-shock was better than control group.

结果 各诱导组诱导家蝇幼虫产生抗菌蛋白达最大活性时间为诱导后36～48h(热诱导后家蝇幼虫很快化蛹，未观察抗菌蛋白产生高峰时间)；金黄色葡萄球菌诱导后的抗菌蛋白抗菌活性最佳，热诱导后的抗菌蛋白抗菌活性也较未诱导组好。

The materials co-cultured with agrobacteriumand cultured transitionally were transferred to screening mediumand 15 adventitious buds were obtained after60d and all of themrooted after they were cultured in rooting mediumfor 20d.

将经农杆菌共培养及过渡培养后的材料转入筛选培养基中，60d后获得15个不定芽，再在生根培养基中培养20d后，全部生根。

In order to unsterdand the properties of rebound and recompression, this study carried out oedometer tests for six soft clays with three surcharge ratios to simulate surcharging. Properties analyzed in this thesis include: 1 post–surcharge secant secondary compression index; 2 amended post- surcharge secant secondary compression index in which the time starts from load application; 3 time at which secondary compression reappears; 4 post-surcharge secondary compression strain rate.

为探讨软弱粘土於超载移除后的回胀与再压缩性质，本研究利用传统单向度压密试验仪对六种土样分别进行三种超载比的超载单向度压密试验，并分析所得结果，分析方法包括：1评估后超载割线二次压缩指数；2评估修正后超载割线二次压缩指数，将后超载的时间原点改为由加载起始时起算；3评估二次压缩再现时间与超载比的关系；4评估后超载二次压缩应变速率之特性。

The herbicides in the sample was extracted with ethyl acetate. The extract was decanted into a flask through a filter paper, then the filtered solution was concentrated to dry. The dry sample was dissolved and added to 5 mL with acetone-n-hexane (30:70, V/V). It was cleaned up by Florisil, concentrated again. The sample was dissolved and adjusted to 1mL with acetone.

匀浆后的草莓试样用乙酸乙酯进行提取，提取液经滤纸过滤后收集，再浓缩蒸干，用丙酮：正己烷(30：70， V/V)溶液溶解至5ml，经Florisil固相萃取柱净化，吹干后用丙酮定容，供气相色谱－质谱联用仪进行测定。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。