后洗流

Results After acute paraquat poisoned thirteen patients appearanced oralis indisposition, nausea, vomit, then appearanced multiple organ functional defect, such as dyspnea, oliguria. Treated with gastric lavage, hemoperfusion, glucocorticoid and so on. Amongst Twelve patients, three patients were cure, seven patients death in hospital, three patients gave up treating then death out of hospital.

结果 13例患者中毒后早期表现为口咽部不适，恶心呕吐，腹痛等，逐渐出现呼吸困难，少尿等各器官受损的表现，予早期催吐，洗胃，血液灌流及糖皮质激素等治疗，13例患者住院1～28d，3例好转，7例院内死亡，3例自动出院后死亡。

Detection the early stage apoptosis cells by flow cytornetry Incubating SCL-1 cells in 6-well culture medium with 1×10~6/well, after 12～48hculture with the concentration of imiquimod 0μg/mL and 150μg/mL, gathering cellsgrowing along the wall and making monocellular suspension, washing in PBS for 2times, and then add 5μL Annexin V/FITC and 10μL PI solutions, reaction for 15min avoiding light, detection the early stage apoptosis cells by flow cytometry.

流式细胞仪测定细胞早期凋亡 SCL-1细胞以1×10~6个／孔接种于6孔培养板中，使用含0μg／mL、150μg／mL咪喹莫特的培养液培养12～48h，收集贴壁生长的细胞制成单细胞悬液，PBS洗两次，分别加入5μL Annexin V／FITC和10μL浓度20μg／mL的PI溶液，混匀后室温避光孵育15min，流式细胞仪检测，结果用Cell Quest软件分析。

For tissue distribution study, rats were sacrified, blood withdrawn and organs including liver, kidney, lung and brain were collected at 10 mins after SR administration.

组织采集则於口服第七剂后 10 分钟时，对大鼠进行灌流洗去全身血液后，采集肝、肺、肾及脑等组织。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。