后柱

BAC filter following ozone contacting column has a better performance than that following air contacting column.

臭氧后生物活性炭柱的运行效果略好于空气后生物活性炭柱。

The chloroform layer and the aqueous layer were separated by multiple column chromatography including macroporous resin, silica gel and polyamide column and preparative HPLC. The compounds obtained were identified by mass spectra and NMR data.

大青叶乙醇提取物，用氯仿萃取后得到氯仿层和水层，各层再分别采用硅胶柱、大孔树脂柱、聚酰胺柱、制备液相、葡聚糖凝胶柱层析及重结晶等分离手段进行分离、纯化，并应用化学性质和质谱、波谱法（1H-NMR、13C-NMR、HMBC）鉴定其化学结构。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

By Vis-UV detector, column efficiencies of conically shaped preparative chromatographic columns with porous polytetrafluor oethylene frit and porous stainless steel frit were compared.

利用柱后可见-紫外检测，比较了采用多孔聚四氟乙烯材料和多孔烧结不锈钢材料作为台锥形制备液相色谱柱柱头筛板材料时的柱效；用柱上可视化方法研究了柱头筛板的直径对流型的影响。

There are some isoforms of PAPs showing inhibitory ability to virus infection in various organs and at different stages of development. Three different species of the protein can be isolated from leaves of pokeweed. Proteins were precipitated with 50-80% ammonium sulfate from leaves in spring, summer and autumn respectively. Proteins were purified on SP cation-exchange then on MQ anion-exchange and finally on MS cation-exchange or on MQ anion-exchange. We found that some new pokeweed antiviral proteins separated from these leaves were different from PAP, PAPII and PAPIII.

对美洲商陆春季、夏季、秋季采集的叶片，用50-80％饱和度硫酸铵沉淀出来的蛋白样品，经过SP阳离子柱后，上MQ阴离子柱，然后把活性蛋白峰再上MS阳离子柱或MQ阴离子柱分离，从春季叶片中分离出四个不同的抗病毒蛋白峰；从夏季叶片中分离出二个不同的抗病毒蛋白峰，这两个蛋白峰与春季叶片中分离出的活性蛋白峰都能重叠上；但从秋季叶片中只分离出来一个抗病毒蛋白峰，它和春季、夏季叶片中分离出的活性蛋白峰都重叠不上。

There are some isoforms of PAPs showing inhibitory ability to virus infection in various organs and at different stages of development. Three different species of the protein can be isolated from leaves of pokeweed (PAP, PAPⅡ and PAPⅢ). Proteins were precipitated with 50-80% ammonium sulfate from leaves in spring, summer and autumn respectively. Proteins were purified on SP cation-exchange then on MQ anion-exchange and finally on MS cation-exchange or on MQ anion-exchange. We found that some new pokeweed antiviral proteins separated from these leaves were different from PAP, PAPⅡ and PAPⅢ.

对美洲商陆春季、夏季、秋季采集的叶片，用50-80％饱和度硫酸铵沉淀出来的蛋白样品，经过SP阳离子柱后，上MQ阴离子柱，然后把活性蛋白峰再上MS阳离子柱或MQ阴离子柱分离，从春季叶片中分离出四个不同的抗病毒蛋白峰；从夏季叶片中分离出二个不同的抗病毒蛋白峰，这两个蛋白峰与春季叶片中分离出的活性蛋白峰都能重叠上；但从秋季叶片中只分离出来一个抗病毒蛋白峰，它和春季、夏季叶片中分离出的活性蛋白峰都重叠不上。

The epoxide groups of the column were modified by a reaction with diethylamine that affords ionizable functionalities required for ion exchange chromatography. The influences of the contents of the cross-linker and porogen on column pressure were investigated.

考察了交联剂及致孔剂用量对色谱柱压力的影响，发现：随着交联剂用量的减小，致孔剂用量的增大，柱压下降；当交联剂和致孔剂用量达到一定水平后，柱压变化不再明显，但柱体机械强度降低。

Keywords: diketoreductase,isomeride,separation and purification,statins chiral side-chain expression, photoaffinity labeling probe

通过DEAE-Sepharose离子交换柱及Sephadex分子筛柱纯化后，酶的纯度达到电泳纯，并通过正相硅胶柱和反相C-18柱分离纯化其反应产物。

As a preliminary theoretical exploration, a limited series of stream function was employed to study the characteristics of the reattachment boundary layer. The tentative analysis showed that the reattachment boundary layer of the circular cylinder, so long as it began at the effective reattachment region discussed above, would be developing to its reseparated point downstream the cross section of the cylinder, which essentially gave the new idea RCDR a theoretical cycle.

本文提出的&再附—减阻&机制可表述为：柱面上扰流元件后向锐缘处诱发的流动分离，既改变了柱面压强分布格局，又破坏了柱面附近的流动状态；若扰流元件锐缘后方流动在圆柱壁面上再附，形成再附边界层，再附边界层将沿流向发展，直至越过柱面最大迎流截面之后，才从圆柱面上分离，致使分离流的尾涡区变窄，绕流阻力降低。

The columns exhibited good separation ability for disubstituted benzene isomers, especially for xylenes and cresols. In addition, the three peralkylated-β-CD columns offer better selectivity than the (2,6-di-O-benzyl)-β-CD column.

同一根柱不同次进样和不同柱之间表现出良好的重复性，保留时间的相对标准偏差小于8.5%；对使用大约40次后的溶胶-凝胶柱重新进行测试，柱效下降不明显，说明该类色谱柱的稳定性良好。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。