后柱

Result: The anticancer active component includes: taxol 9.45%、cephalomanine: 9.63%、10- deacetylbaccatinⅢ: 22.64%、total flavonoids: 19.15%. 3. The preparation of Seiadopitysin control Methods: Take dry Ginkgo leaves and extracted three times by sonication with 95% EtOH, solutions were concentrated under vacuum, after defatting by petroleum benzin, the sample was extracted with EtOAc and the solutions were concentrated under vacuum.

金松双黄酮对照品的制备方法：取干燥银杏叶以95％乙醇超声提取3次，提取液合并，减压浓缩，石油醚脱脂后，用乙酸乙酯萃取，萃取液减压浓缩，经硅胶柱(6cm×80cm)层析，石油醚-乙酸乙酯(9∶1～5∶5)梯度洗脱，产物用甲醇多次冲淋，再以二甲基甲酰氨溶解，反复重结晶，得黄色粉末结晶。

The protein purified with Ni-NTA column was denatured by 8 mol/L urea and dialyzed for refolding.

Ni-NTA柱纯化后的特异性蛋白经8 mol/L脲素变性，梯度透析使蛋白质复性。

The invention uses the fermentation liquor of one Escherichia coli generating polysialic acid as material, filters and removes thallus, adds some solvents as sodium chloride and alcohol into supernatant to deposit polysialic acid, dissolves the polysialic acid deposits via deionized water, filters to remove modified protein, uses alkali prolease to treat filtrate, complexes deposits via cetyl pyridinium chloride or cetyltrimethylammonium bromide, and dissociates, uses over alcohol to deposit polysialic acid, dissolves the deposits via ultra-pure water, grades and purifies via chromatography column, collects and dialyzes eluent, cools and dries to obtain polysialic acid.

本发明以一株产聚唾液酸大肠杆菌的发酵液为原料，经过过滤去除菌体，上清液加入适量氯化钠和乙醇等溶剂沉淀出聚唾液酸，聚唾液酸沉淀用去离子水溶解，再用过滤去除变性蛋白质，滤液经过碱性蛋白酶处理、氯代十六烷基吡啶或十六烷基三甲基溴化铵络合沉淀并解离后，再用过量乙醇沉淀出聚唾液酸，聚唾液酸沉淀用超纯水溶解，用层析柱进行分级纯化，收集洗脱液经过透析，冷冻干燥得聚唾液酸产品。

Methylimidazole in soy sauce was extracted with methylene chloride,diazotized to forms a red colored azo-compound with the highly absorbance at the wave length of 440nm and determined by ultraviolet-visible spectrometry.

酱油样品在层析柱中用二氯甲烷洗脱，经重氮化后在波长440nm处具有最大吸收，据此建立了紫外可见光谱法测定酱油中4-甲基咪唑的方法。

The Sirius pops up lightning fast, since the rainfly remains attached to the canopy when packed (although they can be separated and each used alone), and the poles slide into sleeves on the rainfly. Numerous staking and guying points, an aerodynamic shape, and Exped's tension pole pockets keep the tent rock solid in heavy winds and rain.

Sirius的搭设就有如闪电般的迅速，因为外帐直接与内帐连结在一起(虽然他们可以分开而且可以单独使用)，打开后将营柱穿入外帐的套桶里，在众多的拉撑点以及固定点固定之后，在大风大雨中，EXPED的设计让整顶帐篷如岩石般的坚实强壮。

When the solution had percolated and form ed a narrow initial zone beneath the top of the adsorbent

当溶液过滤后，加在吸附剂上端形成一条很窄的起始环带，新鲜的溶剂被加入，并在柱上加

The satisfactory resolution and selectivity of the polymerized column still remain after conditioned at 250 ℃ for 6 h.

该聚合色谱柱在250 ℃下老化6 h后仍具有良好的分离能力和选择性。

In addition, an improved rough fabrication method called "photo etching" is developed to enhance the milling efficiency for prefabricating the rectangular micropillars which can be polished through the FIB microscope for the finishing step.

压缩变形后SEM观察发现在(01 0)柱面出现平行的滑移线，在(0001)基面出现成60o相交的滑移线。迹线分析表明：当加载面为(2 0)时，两组滑移系(10 0)[1 10]和(1 00)[11 0] 同时开动。

The organic layer was evaporated and the residue was redissolved by mobile phase.The concentrations of propofol and lidocaine were assayed on a hypersil BDS C18 column with a mobile phase consisting of acetonitrile-methanol-water（10∶60∶30）（including 0.14%n-butyamine 0.1%acetic acid）at a flow rate of 1 mL*min-1 and detected at 220 nm during 1～7 min and at 273 nm during 7～16 min.

氮气吹干有机层，残留物用流动相重新溶解后，用Hypersil BDS C18柱分析，流动相为乙腈-甲醇-水(10∶60∶30)，内含正丁胺0.14%和冰醋酸0.1%，流速为1 mL*min-1，检测波长：1～7 min用220 nm，7～16 min用273 nm。

Works out an unit—area method for design of post—tensioned P.

建立了后张预应力砼平板—柱结构设计的单位面积法。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。