后柱

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，將溶酶体的靶向信號肽KFERQ连接在RTA的羧基端；將构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受態大肠桿菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Limestone pillars rear from a temple of the Sabaean moon god Ilumquh.

矗立在供奉塞巴月神伊伦库赫的庙宇后的石灰岩柱。

This method is also available for other problems of unsteady flow around complicated bluff bodies at medium or high Reynolds numbers and the results are used as reference for wake control The phenomena of the sinusoidally oscillating flow past a circular cylinder are also studied.

本文应用有限差分方法求解涡量-流函数形式的N-S方程，数值模拟了园柱瞬时起动后流动分离、旋涡生成、脱落以及随时间推进涡街产生和长期非定常演化过程，包括对称涡的生成，二次涡形成的α结构及卡门涡街等。

Results:77.5% of sphenoid ostia can be identified by endoscope directly.The distances from sphenoid ostia to the superior and lateral wall of sphenoid sinus,the foramen of sphenopalatine artery,the anterior wall of sella,posterior nasal septum and nasal column were 9.6±4.1 mm,9.2±1.8 mm,11.1±1.6 mm,14.7±3.6 mm,3.6±0.5 mm and 61.6±3.7 mm respectively.

结果：77.5%的蝶窦口内窥镜直接窥见，窦口距离蝶窦顶壁、外壁、蝶腭动脉孔、鞍前壁、鼻中隔后端分别为8.6±3.1 mm、9.2±1.8 mm、11.1±1.6 mm、14.7±3.6 mm、3.6±0.5 mm，距离鼻小柱根部前缘61.6±3.7 mm，与鼻底夹角31.8°±5.2°。

After digging basal pit, we found that many piece of rocks were filled between 600 mm below the ground and the surface of river-bed, and the distance between water surface and basal subface is 800 mm.

该站第17道有11个双线路腕臂钢柱基础，经开挖基坑后测得，距地面600 mm以下直至河床面全部为抛填片石，按原设计开挖，则基坑内积水达800 mm。

T739 mice were inoculated with LA795 cells. Angiostatin was given to part of inoculated and uninoculated mice by intraperitoneal injection fourteen days after the inoculation. The treatment lasted 20 days and the size of tumor, survival period, behavior of the mice and pathology of lungs, livers and kidneys were observed.

用亲和层析方法得到的血浆纤维溶酶原经胰蛋白酶消化、层析柱亲和层析、透析，得到纯化的血管抑素；以LA795细胞接种于T739小鼠；接种后第15日起腹腔内注射血管抑素共20 d，观察动物行为，测量荷瘤鼠肿块直径，记录生存期，另设未治疗对照组及观察血管抑素副作用对照组，行肺、肝、肾脏病理学检查。

Methods Affinity chromatography purified plasminogen was digested by pancreatic elastase and angiostatin was obtained by purifying the digestion with affinity chromatography and dialysis. T739 mice were inoculated with LA795 cells. Angiostatin was given to part of inoculated and uninoculated mice by intraperitoneal injection fourteen days after the inoculation. The treatment lasted 20 days and the size of tumor, survival period, behavior of the mice and pathology of lungs, livers and kidneys were observed.

用亲和层析方法得到的血浆纤维溶酶原经胰蛋白酶消化、层析柱亲和层析、透析，得到纯化的血管抑素；以LA795细胞接种于T739小鼠；接种后第15日起腹腔内注射血管抑素共20 d，观察动物行为，测量荷瘤鼠肿块直径，记录生存期，另设未治疗对照组及观察血管抑素副作用对照组，行肺、肝、肾脏病理学检查。

In this paper, by shorting the length of DEAE-Cellulose column and increasing the pH of elution solution, one kind of GO isozyme was purified rapidly from green leaves of spanictu Brassica parachinensis Bailey and Vigna unguiculateW. ssp. sesquipedalis. Verd in nine hours.

本文通过一种改进后的方法，即通过缩短DEAE-cellulose-52柱的长度，以及提高洗脱液的pH，可在9h内从菠菜、菜心和豆角绿叶中纯化GO同工酶。

Improved double disk targets are used to investigate X ray re emission from gold material heated by radiation.The primary disk is an oblique cylinder cavity.X ray radiation emitted from the Au foil in the rear of the cavity ablates secondary Au disk.

采用改进型双盘靶结构，初级改用为斜柱腔结构，激光加热初级薄箔，后向出射的X光烧蚀次级，这样降低初级等离子体喷射的影响，使烧蚀次级的X光更纯净。

IonPac AS9 was selected as the seperation column, 9.0 mmol/L Na2CO3 was eluent. After deposited, decolored and filtrated, the samples was injected and analysized.

采用离子色谱法，选用IonPac AS9分离柱，9.0 mmol/L Na2CO3为淋洗液，水样经沉淀脱色后，过滤进样分析。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。