后柱

Results show that protons are liberated by dehydration or dehydroxylation after calcination at 500℃. These protons disrupt the silicon-oxygen-aluminium bridges where there are Al substitutions in the tetrahedral sheet of the montmorillonite. Some of silicon-oxygen and aluminum-oxygen tetrahedral invert to react with the pillaring agents and yield covalent bonds which firmly fix the pillars to the host clay. The Al-MMT are thermally and hydrothermally stable up to 700℃.

经过500℃高温焙烧后，交联剂经脱水或脱羟基释放出质子攻击蒙脱土四面体层中Si-O-Al四键，硅氧四面体和铝氧四面体发生翻转，与交联剂发生反应生成共价键，形成稳定的层柱黏土材料，在700℃高温下，Al-MMT材料不仅具有较好的热稳定性，而且具有较好的水热稳定性。

In this dissertation , Physical and chemical properties of raw material was studied first, then sodium model-modification of it was researched. Next, preparation of FILM in traditional process using montmorillonite after model-modification was in research. After that, we studied the preparation of PILM in hydrothermal process using montmorillonite without being model-modified. Finally, products in experiments were characterized and Analyzed by XRD, IR, DTA.

根据目前国内外的发展现状，针对存在的问题，本课题做了以下几个方面的研究：首先对采用的基质材料的物化性进行了研究；然后研究了其钠化改型的工艺方法；接下来研究钠化后的蒙脱石用常规工艺合成PILM；在此基础上提出并研究了水热法制备PILM和其柱化剂的新工艺；最后对制备的试样通过XRD、IR、DTA等进行了分析和表征。

Two times hydrolyzation of Fe~(3+) and Cr~(3+) was firstly pointed out in the process of pillar preparation with hydrothermal method. In order to improve the thermal stability, Fe/Cr-Si-pillaring agent solution was synthesized by hydrolyzing Fe~(3+) and Cr~(3+) solution on the surface of silica sol, and Fe/Cr-Si-pillared montmorillonite (Fe/Cr-Si-PILM) was prepared with conventional method and hydrothermal method. Because the technical innovation of preparation is the best way to improve the PILM properties, two novel methods (Microwave method and Ultrasonication method) have been studied to synthesize Fe/Cr-PILM.In order to expand PILMs new application in the field of coal conversion, catalytic activity of Fe/Cr-Si-PILM catalyst in LongKou lignite and ShenHua coal residue liquefaction was discussed and characterized in the last part of this paper.

研究证明：蒙脱石的预处理是可行的，我们以预处理后的Ca-M为原料，研究并优化了PILM的水热法制备工艺，提出了金属离子的二次水解法；为了提高PILM的热稳定性，我们自制了硅溶胶，合成了Fe／Cr-Si三元复合柱化剂，并制备了Fe／Cr-Si-PILM；在PILM合成中，制备技术的革新是改进PILM性能的重要方法，为此我们探索了微波合成法和超声波合成法在制备Fe／Cr-PILM中的应用；最后，我们选择龙口褐煤和神华煤液化残渣为研究对象，探索了Fe／Cr-Si-PILM催化剂在煤炭液化中的应用，希望能开拓PILM应用的新领域。

Cyclization: cyclization occurs at 100℃in polyphosphoric acid for 4h in the mass ratio of 3-(4-methoxyanilino) propionic acid to PPA with 1:8, and 6-methoxy-2,3-dihydro-4-quinolone is formed in 50.5% yield with a method of rapid chromatographic column, c.

成环反应：质量比[3-(4-甲氧苯氨基)丙酸∶多聚磷酸]=1∶8，反应温度为100℃，反应时间4h，柱层析纯化后6-甲氧基-2，3-二氢-4-喹啉酮的收率为50.5％；c。

Lateral crus presented diamond-shaped or long strip,(16.21 ± 2.71) mm in length,(8.45 ± 1.72) mm in width,(1.09 ± 0.18) mm in thickness. Cephalic rim intersected lower edge of lateral nasal cartilage, and slightly covered the lower edge of the lateral nasal cartilage, so that the two were overlapped, but also only the intersection without overlapping. Lateral crus constituted the base of nasal wings. Narrow medial crus formed nasal tip and the frame of front nasal columella, showing posteroinferior curve or S shape,(13.06 ± 2.16) mm in length,(3.79 ± 0.58) mm in width,(1.02 ± 0.18) mm in thickness. The left and right medial crus in the middle were connected by connective tissue, and in the same way connected to the anterior margin of the lateral nasal cartilage.

外侧脚呈菱形或长条形，长(16.21±2.71) mm，宽(8.45±1.72 ) mm，厚(1.09±0.18) mm，头缘与侧鼻软骨下缘相交，并略覆盖侧鼻软骨下缘使二者部分重叠，也可仅相交而无重叠；外侧脚构成鼻翼大部的基础；内侧脚狭细，构成鼻尖和鼻小柱前部的支架，呈向后下的弧形弯曲或S形弯曲，长(13.06±2.16) mm，宽(3.79±0.58) mm，厚(1.02±0.18) mm；左、右内侧脚在正中线借结缔组织相连，并以相同方式连于侧鼻软骨的前下缘。

Affinity chromatography materials for kinase;2. To search a suitable process route of purification of kinase in yeast by affinity chromatography, hexokinase was preparatorily purified by salt preciptation.

为确定一条采用亲和层析技术分离纯化酵母中磷酸激酶的较佳工艺路线，采用分步盐析初步纯化己糖激酶，考察硫酸铵饱争度对盐析效果的影响；以ATP为配基，SESA活化纸纤堆素制备亲和层析剂，确定最佳偶联条件和最佳静态吸附pH值后，将粗酶液直接上亲和层析柱，看分离纯化的效果；在载体和配基之问接上1。

Their pure products of the six compounds were obtained by silica gel column chromatography and recrystallization in propanone solution.

通过硅胶柱色谱分离和重结晶对所得的六种化合物纯化后获得它们的纯化合物。

Methods: After derivatization with FMOC, tarine in beverages was separated and quantitied by HPLC.

以9-氯甲酸药甲酯为柱前衍生剂，与饮料中的牛磺酸发生衍生化反应后，反相高效液相色谱法测定。

Regenerative process was determined by comparing the concentration difference of Na+ in used and unused regenerant, as well as the concentration of Na+ in hydrogen peroxide out of resin columns.

比较浸泡失效树脂前后再生剂中Na+浓度变化和工业级H2O2流经交换柱后Na+浓度，确定适宜的再生方法。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，将溶酶体的靶向信号肽KFERQ连接在RTA的羧基端；将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。