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College of Veterinary Medicine, China Agricultural University, Beijing 100094, China Abstract: A method has been developed to determine residual stilbenes such as diethylstilbestrol, dienestrol and hexestrol in animal tissues using solid phase extraction and gas chromatography-mass spectrometry.

动物组织样品在碱性条件下用乙酸乙酯提取,经碳酸钠溶液液-液分配净化,再用硅胶柱固相萃取净化,洗脱液均分成两份后经氮气吹干,分别用双三甲基硅基三氟乙酰氨和七氟丁酸酐衍生,采用选择离子监测模式进行测定,外标法定量。

We reconstructed the 3-D and virtual endoscopy image about nasal septum, frontal area of the apertura of sphenoidal sinus, sphenopalatine foramen, sphenopalatine artery and sphenoidal sinus use Volume Rendering, Multiplanar Reformation, Virtual Endoscopy and Curved Planar Reformat, and measured and ,about undertaked The nose, nasal septum, sphenopalatine artery, anterior region of the aperture of sphenoidal sinus, sphenopalatine artery and adjacent structures of hypophysial fossa were dissected, observed, measured and analysised the dependency anatomic structure at the same time.

结果: 一、标本解剖 1、鼻孔长径11.32±0.82(10.2~13.2)mm,鼻孔短径7.85±1.21(5.2~9.8)mm。鼻中隔厚度在前、中、后部分别为5.02±0.42(3.8~5.4)mm、5.57±0.47(4.5~6.4)mm、5.55±0.55(4.8~6.4) mm。 2、鼻中隔骨与软骨交界处距前鼻棘21.26±2.47(18.1~27.6)mm,距鼻孔内侧缘中点33.72±3.52(27.1~40.6)mm,距鼻小柱30.12±2.84(26.9~36.8)mm,距蝶窦口下缘38.04±3.49(32.4~45.1)mm,距后鼻孔41.82±3.96(36.9~50.1)mm。四方软骨中部厚度、边缘厚度、前后径、上下径分别为:1.12±0.13(0.92~1.42)mm、1.28±0.24 (0.83~1.67)mm、30.93±4.52(24.9~40.6)mm、29.94±2.14(24.8~34.3)mm。 3、蝶窦口长5.64±0.39(4.92~5.94)mm,宽3.58±0.22(3.10~3.96)mm,距上鼻甲尾部9.5±0.9(8.5~12.1)mm,距上鼻道未端14.5±1.1(12.5~16.4)mm,距中鼻甲尾

Woodson or apocynum venetum Linn as raw material, it utilizes the method of decoction and alcohol sedimentation to remove impurity. After extract is separated by macroporous adsorptive resins, then use polydextran gel LH-20 to purify, obtain isoquercitrin. Compared with the method of traditional silica gel column chromatography, this method has characteristics such as: experiment period is short, dissipation of organic dissolvent is little, yield of produced product is high, and purity coefficient is fine and so on. Isoquercitrin produced by this invention can be provided for medicine research or used as chemistry control article.

Woodson或罗布麻的叶为原料,采用水提醇沉除杂的方法,提取液经大孔吸附树脂分离后再用葡聚糖凝胶LH-20纯化得罗布麻甲素,此法与传统硅胶柱层析的方法相比,具有实验周期短,有机溶媒消耗少,制得的产品得率高和纯度好的特点,本法制得的罗布麻甲素可供药物研究或化学对照品使用。

Gas chromatography, gas-liquid-solid polymer PLOT column, isosteric heats of adsorption

etex碳填充柱中加入1·5%的角鲨烷,通过增加峰的对称性提高了对低分子量碳氢化合物的分辨能力·Bruner等'''首先将石墨化碳黑毛细管柱,用液相改性后制备成石

A method combining HPLC, a post-column derivation device and a fluorescent detector was used to determine residues of ten carbamate pesticides (aldicarb sulfoxide, aldicarb sulfone, oxamyl, methomyl, 3-hydroxy carbofuran, aldicarb, propaxur, carbofuran, carbaryl and methiocarb) in 68 vegetable and 32 soil samples under four different cultivation systems (traditional kailyard, open field vegetable base, green house and paddy) were collected from a certain city in South Jiangsu.

本研究采用高效液相色谱-柱后衍生-荧光检测法分析了苏南某市4种不同种植方式下(传统菜地、露天蔬菜基地、大棚蔬菜基地和水稻田)68个蔬菜样品和32个土壤样品中10种N-甲基氨基甲酸酯类农药(涕灭威、涕灭威砜、涕灭威亚砜、杀线威、灭多威、残杀威、克百威、3-羟基克百威、甲萘威和灭虫威)的残留量,结果表明在蔬菜中检出的氨基甲酸酯农药残留水平多处于0.2~23μgkg^-1,土壤中为0.1~10μgkg^-1;土壤中3-羟基克百威和灭虫威全部检出,其最高检测浓度分别为10μgkg^-1和1.64μgkg^-1,其他农药仅有少数检出。

Methods: NO-3 was restored with cadmium column assay and NO-2 was detected with leavy nitrogen assay. The primitive NO-3 and total restored NO-2(NO-3/NO-2) in plasma of patients with chronic hepatitis and cirrhosis as well as ET-1 with radioimmunology, ALT with Lai's assay were determinated.

取慢性乙型肝炎及活动性肝硬化患者血浆,用镉柱法还原NO-3,重氮法测NO-2,计算血浆中原有及NO-3还原后NO-2总和(NO-2/NO-3),放免法测ET-1和赖氏法测定ALT。

Globin chain analysis proved the α chain to be abnormal The fingerprint of the abnormal α chain and the amino acid sequence of the abnormal peptide obtained indicated that the α 34 leucyl residue had been replaced by arginyl...

将异常α链经柱层析分离纯化后作指纹图,把指纹图的异常肽斑洗脱,用微量双偶合法作氨基酸顺序测定,发现异常肽斑为α34亮氨酸被精氨酸取代,由此证实此异常血红蛋白为Hb Queensα_2~(34Leu→Argβ_2。

The labelling yield of ^99Tc^m-EDDA/HYNIC-Lys^0-TOCA was approxi- mately 96% under the optimized conditions, and the radiochemical purity was more than 99% after Sep-pak column purification.

在优化的标记条件下,^99Tc^m-EDDA/HYNIC-Lys^0-TOCA的标记率约为96%,经Sep-Pak柱纯化后,放化纯度〉99%。

The free radical scavenging effects of Meretrix meretrix hydrolysates prepared with papain,neutral protease and acid protease were investigated.

利用正交实验研究了文蛤蛋白分别经木瓜蛋白酶、中性蛋白酶、酸性蛋白酶作用后酶解物对羟自由基和超氧自由基的清除作用,优化了水解条件,并对最佳清除率下的三种酶解物进行SephadexG50凝胶柱分离,测定酶解物中抗氧化活性肽的分子量分布。

Petrographic examinations, XRD and differential scanning calorimetry analyses of concrete prisms and rock prisms tested were made.

此外,还用XRD、差示扫描量热仪和岩相对岩石柱和混凝土柱膨胀试验后的试体进行了分析。

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