后柱

It is equal to abandoned this part of material of nib lost and sharpens the saw-dust lost with the wooden pencil in fact, Because in 95%, the lead clothing pencil the raw materials utilization ratios of it material-saving the wrapping up core lead part removes the space of blind hole part of filling in backs end of pencil stub that are taken up of post of handle of pencil, The plumbaginous clothing material sharpens the saw-dust equivalent of material lost to consume with the wooden pencil basically, The pencil utilization ratio of raw materials produced with the timber is in 30-35%, a finished product pencil can only use two-thirds in using, Back one-third is abandoned, so the material-saving pencil is still pencil province of material-saving raw materials compared with wooden pencil.

被弃掉的笔头这部分材料实际上是与木制铅笔削掉的木屑是相等的，因为，节材铅笔它的原材料利用率在95%，铅衣包铅芯部分除去铅笔柄柱塞所占的铅笔头后端的盲孔部分的空间，铅衣材料基本和木制铅笔削铅衣削掉的木屑材料等量耗费的，而用木材生产铅笔的原材料利用率在30%-35%，一支成品铅笔在使用中只能使用三分之二，后三分之一被弃掉，所以节材铅笔与木制铅笔相对比还是节材铅笔节省原材料。

A method of high performance liquid chromatography with post-column derivatization and terbium sensitized fluorescence for simultaneous determination of residues of ciprofloxacin, norfloxacin and enrofloxacin in chicken was established, based on the effect that Tb3+ can sensibilize fluorescence of fluoroquinolones.

基于氟喹诺酮类药物与铽离子形成配合物后的荧光增强作用，建立了同时检测鸡肉中氟喹诺酮类药物环丙沙星、诺氟沙星和恩诺沙星残留的Tb3+增敏高效液相色谱柱后衍生荧光检测方法。

Results: The distances from the root of columella nasi to the inforior pole of sphenoidal sinus aperture and the sphenopalatine foramen were (60.40±3.21,58.10-72.76) mm and (62.14±1.93,59.50-73.40) mm, respectively.

结果：鼻小柱根部至蝶窦口下极及蝶腭孔的距离分别为(60.40±3.21，58.10~72.76)mm和(62.14±1.93，59.50~73.40)mm；蝶腭孔至蝶窦口下极的距离为(12.20±1.10，8.10~16.35)mm，在蝶腭孔处蝶腭动脉的外径为(1.99±0.13，1.50~2.80)mm；蝶窦口下极至鼻中隔后上动脉及鼻中隔后下动脉的距离分别为3.49±0.24，2.78~5.20）mm和(6.42±1.08，4.30~8.50mm。

The results showed that pH of the irradiated sediment core is higher than the unsterilized one,and microorganisms could have an effect on the pH of sediments.All the four sediments are in the strong reductive environment at the depth of 7～30 cm,and the values of Eh are quite closer,the main factor that affect the redox environment may be the compaction of the sediment.All the sediments had a cumulation of phosphorus at the surface layer,and the total phosphorus of the sediments at the depth of 4～10 cm are lower than the average.It is indicated that phosphorus had been redistributed at the suface layer.

本研究采集太湖梅梁湾沉积物，通过印Co.y射线辐照、添加可溶性淀粉等处理研究微生物活性对太湖沉积物pH，Eh及总磷分布影响，以揭示微生物在湖泊沉积物中的沉积后作用，为探索富营养化湖泊表层沉积物磷的归趋提供理论基础。1 材料与方法1.1 样品采集和处理2005年1 1月，采用自制的柱状采泥器采集太湖梅梁湾沉积物，采样位点为31.30.306 N.120.13.370 E，采集后立即带回实验室，其理化性质见表1。

A fully automated simultaneous determination of nitrate and nitrite in water samples by sequential injection analysis based on the Griess diazo-coupling reaction and the formed azo dye for nitrite at 540nm and reduction to nitrite using coppered cadmium column.

用顺序注射分光光度法，通过一次进样。同时测定硝酸根和亚硝酸根。亚硝酸根是根据格里斯重氮－偶联反应，形成偶氮染料后在波长540nm下进行检测。而硝酸根则通过镀铜镉粒还原柱还原为亚硝酸根后进行测定。

Through a variety of resin filter, adsorption and elution conditions for the optimization, as well as decolorization, crystallization methods of research, this article eventually determine the strong acid cation resin HZ-001 which is at pH 3, 3BV / h, having the largest adsorption to the tryptophan; the fermentation broth were centrifuged to remove sediment,then treated with activated carbon to be decolored and then treated with strong acid cation resin HZ-001. After that,2mol/l amino acid were passed through the HZ-001 ion exchanging column,kept almost constants when the flow rate stayed 6 BV / h. At 60℃,it was condensed into the saturated state and then added Ethanol until its percentage reaching 30%. At 4 ℃ under 100rpm placed in the shaker 20h, the collection of L-tryptophan was dried up in vacuo.

本文通过对种树脂的筛选、吸附和洗脱条件的优化，以及对脱色、结晶方法的研究与摸索，最终确定强酸型阳离子树脂HZ-001在pH=3，3倍床体积流速，温度室温，对色氨酸有最大的吸附作用；将发酵液离心去沉淀并用活性炭脱色后通过HZ-001强酸型阳离子交换树脂柱，再用2mol/L的氨水6倍床体积流速在室温下进行洗脱，收集洗脱液，60℃浓缩为过饱和状态后加入30%无水乙醇，4℃下100rpm摇起晶20h，过滤搜集结晶并真空干燥得白色粉片状L-色氨酸晶体。

The sample was extracted by deionized water. The extract was cleaned up and concentrated on a SPE column, then eluted by methyl-tert-butyl ether. The elution was concentrated under nitrogen and redissolved to 1 mL.

样品中24种目标物用蒸馏水浸出后，经固相萃取小柱富集净化，再用叔丁基甲醚洗脱，洗脱液氮吹浓缩定容后用气相色谱－质谱法测定。

An elution process is monitored at the position of a wavelength of 363nm on an ultraviolet spectrophotometer to collect a solution at a first wave crest and after the solution is cooled and dried for 22 hours to 28 hours, folacin coupled chitosan solid is obtained.

将反应后的产物冷却至室温，用凝胶柱进行分离，在紫外分光光度计上，于363nm波长处监测洗脱过程，收集第一个波峰的溶液，将其冷冻干燥22～28小时后，得到叶酸偶联壳聚糖固体。

Finally, wetested its antibacterial activities by E.coli and S.aureus.Methods In this research, the recombinded gene was cloned into the secretion vector pTYB12 and expressed in the E.coli BL21DE3. We did the preliminary research on secretion-expression of the gene. During the purification procedure, we used the chintin beads to wash off the sundry protein, then dialysed the collecting liquid to get rid of the salinity and the other small molecular peptides.

将上述重组基因抗菌肽的氨基酸片段克隆并拼接到分泌表达载体pTYB12上转染大肠杆菌(E.coli BL21DE3)后进行分泌表达，破碎离心收集的诱导表达大肠杆菌，首次应用几丁质珠柱分离纯化系统进行分离纯化，分离洗脱杂蛋白后透析去除盐类物质及其它小分子肽，得到目的重组基因抗菌肽。

The IWFs were purified by (NH4)2SO4 precipitation fractionation, and then applied to Sephadex G-75 column. The column eluates were collected separately according to A280 nm and injected into the health wheat leaves to examine the eliciting activities by PAL, PO assays.

IWF经硫酸铵粗沉淀后，将蛋白沉淀用重蒸水重新溶解后进行凝胶过滤柱层析，以280nm紫外吸收检测、收集不同的蛋白峰，分别注射到健康的Thatcher叶片中，以苯丙氨酸解氨酶(phenylalanine ammonialyase，PAL)、过氧化物酶(peroxidase，PO)防卫反应作为评价诱导活性的指标，检测活性成分在各部分中的分布和诱导活性的强弱。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。