后效

The ELISA was conducted to detect the antisera of mouse and presented good results.

间接ELISA检测结果显示，Tc-BSA、Tc-HSA两种连接物在四免后抗体效价均达到1：3200以上。

The xinguang tube time-sensitive, relatively short exposure time, relatively small light permeability to expand; however, as the UV light to rule over the ages, lighting effects, exposure time gradually extended, fine idea is not easy, but deep PIP light permeability to expand it is becoming increasingly damageto, resulting in a traditional version that are the same pair of film on the same printer, after a certain period of time, get the flexo from deep network to a brief network halftoning, also occur when different curves change!

这表示在新平惯时平敏不弱，曝平工夫相差长，平渗放大相差小；但随着UV平惯的忽然老化，平效放松，曝平工夫忽然延不幼，粗不面子不便当不入来，但浅不面子的平渗放大愈发狠恶，酿成古板版那怕是统一副胶片在统一台晒版机上，经过一段期间后，晒不入的刚性版从浅网到浅网的阶调，也会爆发差别的弧线改变！

Attention: Shengji Water should meet during the day and full-cream effect protective cream or sunscreen use, not imported, eye, skin-allergic, to be used in our ears after the test-min.

白天应配合生肌补水霜和全效防护霜或防晒霜使用，勿入口、眼内，易过敏性肌肤，使用时须在耳后试敏。

If there were no signs and symptoms of spinal anesthesia or toxic systemic effect, 12ml 3% chloroprocaine hydrochloride or 2% lidocaine were given five minutes later. The onset times of cold free and pain free, the peak block height and it\'s onset time, the recovery times of pain and motor were recorded; vital signs (including BP,HR and SpO2) at the beginning of anesthesia and 5min,10min,15min,20min ,30min later were recorded, as well as the ephedrine consumption, oxytocin consumption, Apgar scores of newborn, satisfactory degree of uterine contraction and side effects.

以第二次给药作为起始时间点，记录麻醉起效时间，痛觉消失时间，最高阻滞平面及时间，痛觉恢复时间，运动恢复时间；评价手术肌松效果；记录麻醉前，麻醉后5min，10min，15min，20min，30min的血压，心率和SpO2；术中麻黄碱用量；新生儿出生1分钟，5分钟，10分钟的Apgar评分；缩宫素用量和子宫收缩情况以及不良事件的发生情况。

Based on the analysis of formation mechanism of shrinkage, dispersed shrinkage, gas cavity and blow hole shrinkage in the brake hub castings of Santana car, some methods are put forward to solve these problems.

本文在对桑塔纳轿车后制动毂的缩松、缩孔、气孔、气缩孔等孔洞类缺陷进行机理分析的基础上，提出了一些解决的措施，并在实际生产中取得了明显的效

The titer degree of this hybridoma cell line remained unchanged after frozen in liquid nitrogen for three months.

将杂交瘤细胞株在液氮中冻存3个月后复苏，其分泌的单克隆抗体效价不变。

The isotypes of the five anti-CT mAbs were identified as IgM heavy chain and λ light chain. For the purification of anti-CT mAb, Hitrap IgM Purification Column was used.

其中CT-3A与CT-9A单株抗体经大量生产以Hitrap IgM Purification Column纯化后，经间接型ELISA免疫分析抗体效价在1：15，625。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

Results and ConclusionThe study of time-efficacy relation expresses that after infusing the solution of Phyuanthus emblica Phyllanthus emblica, medicated serum in different time activates the spleen lymphocyte of small mice differently after the 30min but before 180min. During this period, on 2h the activation of the medicated serum is the most obvious. The quantity-efficacy relation research expresses that megadose promotes the propagate of the spleen lymphocyte of small mouse and activates the macrophage in the abdominal cavity more obviously. On the other hand, the small dosage influents the survival rate of the mouse S180 ascites tumor cell more obviously.

结果与结论时效关系研究表明，用余甘子冻干粉溶液灌胃后30～180 min内不同时相含药血清对小鼠脾淋巴细胞具有活化作用，并在给药2 h时实验血清活化作用明显；量效关系研究表明，余甘子大剂量组实验血清对促进小鼠脾淋巴细胞增殖，促进小鼠腹腔巨噬细胞细胞活化作用以及小剂量组实验血清对小鼠S180腹水肿瘤细胞存活率的影响均具显著差异性。

Microwave guideline in effect in 1970 for the public, 10,000 microwatts/^2 (now the industrial and military "guideline"), is proscribed from consideration of the rate that thermal effects are dissipated, the author, a specialist in modeling neural networks, states that scientific studies have consistently shown that humans exhibit behavioral disturbances when subjected to non-thermal levels of microwaves, well below this level.

MacGregor 发表了一篇标题为&低能量的微波对神经中枢的作用的简述&，在美国在1970年起效了&微波的条律&后， 1000微瓦/每平方米的微波密度被禁止，作者，一个专门研究神经网络的的科学家，陈述，各种研究表明这个标准浓度以下的微波辐射将对人类的行为将产生骚乱影响。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。