后成质

OBJECTIVE: To label rabbit BMSCs by green fluorescent protein, and then to observe the potency of multi-directional differentiation by osteoblast and lipoblast formation induction.

目的：采用绿色荧光蛋白标记兔骨髓间充质干细胞，观察标记后其诱导成骨及成脂能力。

The process is carried out by rubbing into filament raw materials, digesting in plasticizing liquid of sodium hydrate and sodium silicate at 100-130degree for 1-2hrs to obtain plasticized raw material, high-speed agitating plasticized raw materials with non-metallic minerals, octadecanoic acid and silicon oil at 100-130degree for 15-45hrs, coating and drying to obtain the final product.

先用农用搓揉机直接搓揉成丝状物料，然后丝状物质在含氢氧化钠和硅酸钠的塑化液中于100～130℃下蒸煮1～2小时，得到塑化物料，最后将塑化物料同改性非金属矿粉、硬脂酸和硅油在100～130℃下高速搅拌15～45分钟进行包覆处理，干燥后便得到目标产物。

RESULTS: High-purity ADSCs were acquired successfully. The ADSCs were induced into osteoplastic differentiation, and displayed typical adipocytes changes 7 days after induction. the cells turned to slender fusiform and mixed together, which showed positive PPARγ immunohistochemical staining and the lipid droplet was stained orange-red by oil red staining.

结果：①成功获得纯度较高的脂肪基质细胞，成功诱导其脂向分化，诱导培养后7 d观察到细胞形态学上表现出典型的脂肪细胞改变：细胞相互融合，成片生长，变为细长纺锤形；PPARγ免疫细胞化学染色呈阳性；油红O染色脂滴被染成橙红色。

Studies on the regulation of malignant phenotype expression in a mouse plasmocytoma cell line (SP2/0) by means of cellular engineering techniques of cell hybridization between the fusion of the mouse myeloma cells with rat intermediate or late erythroblasts.

三、利用细胞杂交的细胞工程手段，首次系统地研究了大鼠中幼、晚幼成红细胞与小鼠浆细胞瘤（SP2/0）细胞融合后恶性表型的表达状态，分析自然去核前红细胞胞质对骨髓瘤细胞恶性增殖的调控作用，以及晚幼期固缩核重新被激活的可能性。

The goat fetal fibroblast cells were transfected by linear GBCt-PAm with electroporation or lipidosome, and after the identification of PCR, we got 7 lines of progenitive cells that examined positive.The two cell line (M9, M10) had been used for the somatic nuclear transfer for the first time.

将pGBCt-PAm去除原核序列后回收线性片段，采用电击和脂质体转染两种方式转染山羊胎儿成纤维细胞，经G418筛选培养基筛选及PCR检测，最终得到了7株能有效扩增的PCR检测阳性的细胞克隆。

The replicons were detected and characterized by RT-FUR. IFA. Renilla Luciferase assay system and real time RT-FUR. respectively. Results It was shown that SLB2 mutant did out significantly affect the translation of the input RNA.

将突变体（包括相关的阳性和阴性对照复制子）分别线性化并体外转录成RNA后，取等量各转录体RNA，以脂质体法分别转染BHK-21细胞。

Finally, the article discussed the possible mechanism of multi-phase electrochemical degradation of wastewater through the XPS spectrum of the used catalysts surface: In the acid solutions, the electro-generated O_2 may get electrons on the porous graphite cathode and then form H_2O_2, H_2O_2 may diffused to the surface of catalyst and reacted with its active part to produce OH, which in turn can destruct organics. The catalyst functioned as both the catalyst to decompose H_2O_2 and the flocculants to sedimentate the degraded waste.

最后全文结合研究反应后催化剂表面的XPS光电子能谱推断了该体系降解污废水可能的反应机理：在酸性条件下，石墨双阳极产生的O_2被多孔扩散性石墨阴极还原成H_2O_2，并通过扩散传质作用在催化剂的表面活性中心分解为强氧化性中间产物·OH，强氧化性攻击有机物，使其降解为小分子有机物或CO_2和H_2O，催化剂在体系中即起催化作用也起吸附、絮凝作用。

The invention relates to a pipeline polyurea outer anticorrosive coating and the making method thereof, making heated prepolymerization reaction on dibenzo-methane diisocyanate, carbodiimide diisocyanate, polyether binary alcohol and polyether trihydric alcohol to prepare prepolymer solution A, and making end amino polyethylene oxide ether, dimethyl sulfur-based toluene diamine, diethyl diamine, iso-propyl titanate solution, polyacrylate, carbon black, titanium dioxide and lightweight calcium carbonate into solution B, and as using, respectively heating the solutions A and B to 65 deg.C and then mixing them, then quickly spraying and curing to form a coat.

本发明涉及一种管道用聚脲外防腐涂料及制造方法，由二苯基甲烷二异氰酸酯、碳化二亚胺化二异氰酸酯、聚醚二元醇和聚醚三元醇通过加热预聚反应制成预聚物的A液、由端氨基聚氧化乙烯醚、二甲硫基甲苯二胺、二乙基甲苯二胺、钛酸异丙酯溶液、聚丙烯酸酯溶液、碳黑、钛白粉和轻质碳酸钙配制而成的B液组成，使用时将A液和B液分别加热至65℃后混合，然后快速喷涂固化形成涂层。

After special chemical process,it wi8ll inflate in to wormlike when expandable graphite in high temperature for an instant.

经过特殊化学处理后遇高温可瞬间膨胀成蠕虫状的天然晶质石墨。

ZHAO Yede*,HE Qinglian.Department of Plastic Surgery, Navy 411 Hospital, Shanghai 200081Objective To investigate the expression level and intracellular distribution of calreticulin in hypertrophic scar-derived fibroblastsand normal skin-derived fibroblasts.

增生性瘢痕(Hypertrophic scar， HS)作创伤及外科手术后常见且十分棘手的皮肤组织疾病，瘢痕组织内成纤维细胞异常活化和细胞外间质过度沉积既是HS的重要组织学特征，也是HS发生、发展的物质基础。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。