后成质

This thesis try to discuss what kind of knowledge-diffusion model and knowledge-integration model would easily success in different innovation type from knowledge-based theory. The research methodology is case study of quality method and research target is knowledge intensive industry. The research has interviewed eight managers and concluded four case. Finally, we have some conclusion below

本研究尝试从知识基础论的角度出发来探讨在不同的创新类型之间，应采用何种的知识流通模式与知识整合机制较容易成功，研究方法采用质性研究之个案访谈法，研究对象为知识密集产业，总共访谈了八位高阶主管，并将之整理成四个个案，经由分析归纳后得到以下几点结论。

Results The mutation mouse model was established successfully, and inflammatory reaction such as bacterial infection、inflammatory cell infiltration、proliferation of fibroblast and neogenesis capillaries of neonatal mouse model can be observed with TEM in different stage.

结果 成功建立小鼠模型；在模型小鼠出生后的不同时段中，电镜下小鼠角膜基质内可见有细菌感染、炎症细胞浸润、成纤维细胞及新生毛细血管增生等炎症改变。

The distribution of protium and deuterium in zirconium hydride films are analyzed by using depth profiles and images of SIMS.

电阻蒸发制备的锆膜氢化后，利用二次离子质谱对其进行了深度剖析与成像分析，结果表明氕与氘在锆膜深度分布均匀，在过渡层中呈递减分布，并消失于过渡层与衬底的交界处。

Solute chitosan into acetic acid, stir them to uniformity cystose , refrigerate and cast form into porosity sponginess wire frame..

将壳多糖经乙酸溶解后，搅拌成均匀泡沫状，冷冻铸型制成海绵状多孔基质网架。

Then the caprine fetal fibroblast cells were transfected with p6.5hLF-EGFP by LipofectamineTM-2000 and selected by G418 for 3 to 4 weeks. The G418 resistant transfectants were identified by PCR and EGFP detection. The results indicated that the transgene was stably integrated into the open region of the chromatin of G418 resistant fibroblast cells.

脂质体介导法转染山羊胎儿成纤维细胞，G418抗性筛选3-4周后，经PCR扩增和报告基因EGFP表达检测，得到稳定整合外源基因的转基因供体细胞系，为制备高效表达人乳铁蛋白的转基因山羊乳腺生物反应器提高可靠的核移植供体细胞。

Bone marrow containing hematopoietic stem cells has already been known , theses another group stem cells exists in bone marrow named mesenchymal stem cells. MSCs retain the potentiality of differentiating into osteoblast, chondroblast, adipoblast, tendonblast, myoblast, cardiomyoblast, neuron, etc , which displays a bright prosperity of its use in tissue enigeering, wound healing , cell transplatation and gene therapy. MSCs can be obtained and expanded easily compared to ESC without any problems of morality , law and ethic , immunology.

最早在130年前，德国病理学家Cohnheim在研究创口愈合时就推测在创口愈合过程中出现的部分细胞可能来自骨髓。20世纪70年代，Friendenstein发现一小部分骨髓中贴壁最牢固的细胞经数此传代后形成独特的纺锤形外观，具有形成成骨细胞或软骨细胞的能力。80年代以来，许多研究小组进一步证实了Friendenstein分离到的这些细胞是多能的，在体外特定诱导条件下，可以分化为成骨细胞、软骨细胞、脂肪细胞、成肌细胞、神经元等，被称为骨髓间充质干细胞(Bone MarrowMesenchymal Stem Cells，MSCs)。

Methods Trizol reagent from clamworm digestive tract epithelial cells extracted methods Total RNA. The mRNA was purified and reverse transcripted into cDNA. The cDNA was linked into plasmid pDNR-Lib after digested by nucleate endonuclease Sfi I to construct cDNA library.

分离沙蚕消化道上皮细胞，使用Trizol试剂提取总RNA，纯化mRNA，用MMLV逆转录成cDNA，用Sfi I核酸内切酶酶切后连接到pDNR-Lib质粒中，构建cDNA文库。

Pedicel covered by bracteoles; bracteoles and sepals imbricate,± caducous after anthesis, crustaceous, margin scarious.

小苞片盖住的Pedicel；小苞片和萼片成鳞状，±早落的在花后，壳质，边干膜质。

Results After used the SGS , the arrange of collagen tend to unifying , micrangiums are rich and in opening condition, the polymeride of pre-collagen accumulate in cytochylema in fibroblast, the total content of collagen is decreased, but the content of hPcⅢ is kept in a high level, the content of HA is increased gradually,which closes to the normal skin level.

结果 应用硅凝胶膜后，胶原排列趋于一致，微血管丰富而呈开放状态，成纤维细胞的前胶原聚合体蓄积在细胞浆内；胶原总的含量减少，但Ⅲ型前胶原含量维持在一个较高水平；透明质酸含量逐渐升高，接近正常皮肤水平。

Induct cultured cells to differentiate into endothelial cells、lipoblast and osteoblasts in vitro.

对鉴定后的胎盘源间充质干细胞在体外通过特定诱导环境下使之向成脂肪细胞、成骨细胞分化，检测其多向分化潜能。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。